Screening target genes for bimolecular marking methods of Magnolia quality

Abstract

The study used use bimolecular marking methods to evaluate the lignans of Magnolia officinalis and M. officinalis var. biloba. First, we compare the chemical constituents between M. officinalis and M. officinalis var.biloba. There were significant differences in concentration of magnolignan I between leaves of these two varieties. Then we further select the p-hydroxyphenyl lignin to mining the key enzyme genes of biosynthesis from Magnolia transcriptome, and screened an encoding cinnamyl alcohol dehydrogease gene as the candidate marker of bimolecular marking methods of Magnolia quality by comparing of the expression level and structure variation in homologous gene between M. officinalis and M. officinalis var.biloba. The established method provides the technical support for bimolecular marking methods of Magnolia quality evaluation.

Transcriptomic Analysis Reveals Adaptive Evolution and Conservation Implications for the Endangered Magnolia lotungensis

“…The de novo assembly of the transcriptome yielded a robust set of 177,046 transcripts with 42,518 coding sequences (CDSs) identified, of which 37,271 (87.7%) were functionally annotated. Although transcriptome sequencing may not be the most optimal technique for genetic exploration today, given the critically endangered status of M. lotungensis and the difficulty in obtaining samples, the quality of the assembled transcriptome is sufficient to provide a comprehensive description of the species' genetic information, and are comparable to previous studies focused on the Magnolia [15,16,[34][35][36]. For instance, the de novo transcriptome assembly of M. wufengensis and M. champaca yielded 59,764 and 47,688 non-redundant unigenes, respectively [15,16].…”