Screening siRNAs targeting a novel gene (HA117) and the development of a derivative recombinant adenovirus delivery system

Zheng, Gaihuan; Luo, Qing; Jin, Xianqing; Guo, Yuxia; You-hua, XU

doi:10.1038/cgt.2011.32

Cited by 2 publications

(1 citation statement)

References 15 publications

(20 reference statements)

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“…To determine whether Shn3 is an essential mediator in BMP9-induced osteogenic signaling, we constructed a recombinant adenovirus that expresses a pool of three small interfering RNA (siRNA) targeting human Shn3-coding regions using the established pSOS system as recently described 36 , which prompted Ad-Sim-Shn3 to efficaciously knock down Shn3 expression in hAMSCs. After transfecting the cells with Ad-RFP, Ad-BMP9, and Ad-Sim-Shn3 for 24 h, cells were observed by fluorescence microscope (Fig.…”

Section: Silencing Shn3 Expression Potentiates Bmp9-induced Osteogenimentioning

confidence: 99%

RETRACTED ARTICLE: Schnurri-3 regulates BMP9-induced osteogenic differentiation and angiogenesis of human amniotic mesenchymal stem cells through Runx2 and VEGF

Liu

Tang

et al. 2020

Cell Death Dis

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Human amniotic mesenchymal stem cells (hAMSCs) are multiple potent progenitor cells (MPCs) that can differentiate into different lineages (osteogenic, chondrogenic, and adipogenic cells) and have a favorable capacity for angiogenesis. Schnurri-3 (Shn3) is a large zinc finger protein related to Drosophila Shn, which is a critical mediator of postnatal bone formation. Bone morphogenetic protein 9 (BMP9), one of the most potent osteogenic BMPs, can strongly upregulate various osteogenesis-and angiogenesis-related factors in MSCs. It remains unclear how Shn3 is involved in BMP9-induced osteogenic differentiation coupled with angiogenesis in hAMSCs. In this investigation, we conducted a comprehensive study to identify the effect of Shn3 on BMP9-induced osteogenic differentiation and angiogenesis in hAMSCs and analyze the responsible signaling pathway. The results from in vitro and in vivo experimentation show that Shn3 notably inhibits BMP9-induced early and late osteogenic differentiation of hAMSCs, expression of osteogenesis-related factors, and subcutaneous ectopic bone formation from hAMSCs in nude mice. Shn3 also inhibited BMP9-induced angiogenic differentiation, expression of angiogenesis-related factors, and subcutaneous vascular invasion in mice. Mechanistically, we found that Shn3 prominently inhibited the expression of BMP9 and activation of the BMP/Smad and BMP/MAPK signaling pathways. In addition, we further found activity on runt-related transcription factor 2 (Runx2), vascular endothelial growth factor (VEGF), and the target genes shared by BMP and Shn3 signaling pathways. Silencing Shn3 could dramatically enhance the expression of Runx2, which directly regulates the downstream target VEGF to couple osteogenic differentiation with angiogenesis. To summarize, our findings suggested that Shn3 significantly inhibited the BMP9-induced osteogenic differentiation and angiogenesis in hAMSCs. The effect of Shn3 was primarily seen through inhibition of the BMP/Smad signaling pathway and depressed expression of Runx2, which directly regulates VEGF, which couples BMP9-induced osteogenic differentiation with angiogenesis.

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Section: Silencing Shn3 Expression Potentiates Bmp9-induced Osteogenimentioning

confidence: 99%

RETRACTED ARTICLE: Schnurri-3 regulates BMP9-induced osteogenic differentiation and angiogenesis of human amniotic mesenchymal stem cells through Runx2 and VEGF

Liu

Tang

et al. 2020

Cell Death Dis

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The novel gene HA117 promotes in vitro and in vivo drug resistance in mouse colon tumor cells

et al. 2017

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In this study, we characterized the role of HA117, a novel gene we previously identified, in drug resistance in vitro and in vivo. Briefly, CT26 cells expressing HA117 were obtained by infection with a recombinant adenovirus, and tested for drug sensitivity by methyl thiazolyl tetrazolium assay. In addition, the effect of HA117 on drug sensitivity was assessed in CT26 colon tumors transplanted into nude mice. In vitro, expression of HA117 increased the resistance of CT26 cells to 5-fluorouracil 2.75 times (P<0.05). Similarly, expression of HA117 in subcutaneously xenografted CT26 colon cancer significantly lowered its sensitivity to 5-fluorouracil (P<0.05). Collectively, the results suggest that HA117 regulates the development of drug resistance in CT26 cells in vitro and in vivo.

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Screening siRNAs targeting a novel gene (HA117) and the development of a derivative recombinant adenovirus delivery system

Cited by 2 publications

References 15 publications

RETRACTED ARTICLE: Schnurri-3 regulates BMP9-induced osteogenic differentiation and angiogenesis of human amniotic mesenchymal stem cells through Runx2 and VEGF

RETRACTED ARTICLE: Schnurri-3 regulates BMP9-induced osteogenic differentiation and angiogenesis of human amniotic mesenchymal stem cells through Runx2 and VEGF

The novel gene HA117 promotes in vitro and in vivo drug resistance in mouse colon tumor cells

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