Screening seeds of some Scottish plants for free radical scavenging activity

Kumarasamy, Yashodharan; Byres, Maureen; Cox, Philip; Jaspars, Marcel; Nahar, Lutfun; Sarker, Satyajit D.

doi:10.1002/ptr.2129

Cited by 242 publications

(176 citation statements)

References 26 publications

Supporting

Mentioning

158

Contrasting

Order By: Relevance

“…The free-radical-scavenging activity of the extracts, SPE fractions and isolated glycosides (1-3) were determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical-scavenging assay (Kumarasamy et al, 2002(Kumarasamy et al, , 2007 (Table 3). The MeOH extract showed the highest level of activity with a RC50 value of 1.15 ×10 -1 mg/mL among all extracts, and most of its activity was contributed by the SPE fractions of 40% and 60% aq.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

Delazar

Delnavazi

Yassa

et al. 2012

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

Abstract:From the methanolic extract of the aerial parts of Ajuga chamaepitys (L.) Schreb., Lamiaceae, one of the Iranian medicinal plants, the phenylethanoid glycoside, acteoside, and two flavone glycosides, chrysoeriol 7-Oglucopyranoside (3'-methoxy-luteolin 7-O-glucopyranoside) and apigenin 7-Orhamnopyranoside, were isolated by a combination of solid-phase extraction (SPE) and preparative reversed-phase high-performance liquid chromatography (prep-RP-HPLC) methods. Structures of the isolated compounds were elucidated by spectroscopic means. The free-radical-scavenging properties of the extracts, fractions and isolated compounds were determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. While among the extracts, the MeOH extract showed the highest level of free-radical-scavenging activity (RC50 1.15 × 10 -¹ mg/mL), chrysoeriol 7-O-glucopyranoside was the most active (RC50 3.00 × 10 -³ mg/mL) among the isolated compounds. The GC-MS and the GC-FID analyses revealed α-pinene (23.66%), β-pinene (9.33%), 1-octen-3-ol (9.72%), β-phellandrene (8.70%) and germacrene-D (7.92%) as the major components of the essential oils derived from the aerial parts of this plant. The presence of phenolic glycosides and the α-and β-pinene-rich essential oils in A. chamaepitys may provide some rationale for the traditional medicinal uses of this species in Iran.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The free-radical-scavenging effect of the extracts, fractions and isolated compounds was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay (Kumarasamy et al, 2002(Kumarasamy et al, , 2007. DPPH was obtained from Fluka Chemie AG, Bucks and a solution of DPPH (0.08 mg/mL) in methanol was used.…”

Section: Gc-ms and Gc-fid Analysesmentioning

confidence: 99%

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

Delazar

Delnavazi

Yassa

et al. 2012

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The antioxidant potential of 1-5 was evaluated by the DPPH assay (Kumarasamy et al, 2007). In the qualitative DPPH assay, except 3, all compounds showed extremely low levels of antioxidant activity by displaying faint white spots against a purple background on the TLC plates.…”

Section: Resultsmentioning

confidence: 99%

“…The method used by Takao et al (1994) was adopted with suitable modifi cations (Kumarasamy et al, 2007). DPPH (8 mg) was dissolved in MeOH (100 mL) to obtain a concentration of 80 g/mL.…”

Section: Antioxidant Assaymentioning

confidence: 99%

Coumarins from the aerial parts of Prangos uloptera (Apiaceae)

Razavi¹,

Nazemiyeh²,

Hajiboland³

et al. 2008

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

RESUMO: "Cumarinas das partes aéreas de Prangos uloptera (Apiaceae)". Os estudos fi toquímicos das partes aéreas de Prangos uloptera, uma espécie do gênero Prangos endêmica do Irã, forneceram cinco cumarinas, xantotoxina (1), prangenina (2), escopoletina (3), deltoína (4) e prangolarina (5). As estruturas destas cumarinas foram elucidas através de métodos espectroscópicos e o potencial antioxidante de 1-5 foi avaliado pelo ensaio de DPPH. O signifi cado quimiotaxonômico de 1-5 também é discutido.Unitermos: Prangos uloptera, Apiaceae, Umbelliferae, cumarina, furanocumarina, marmesina, antioxidante, quimiotaxonomia.ABSTRACT: Phytochemical studies on the aerial parts of Prangos uloptera, an endemic Iranian species of the genus Prangos, yielded fi ve coumarins, xanthotoxin (1), prangenin (2), scopoletin (3), deltoin (4) and prangolarin (5). The structures of these coumarins were elucidated by spectroscopic means, and the antioxidant potential of 1-5 was evaluated by the DPPH assay. The chemotaxonomic signifi cance of 1-5 is also discussed.

show abstract

“…The ability of the plant extract to scavenge 1,1-dyphenyl-2-picrylhydrazyl (DPPH) free radicals was assessed using the method described by Kumarasamy et al [11]. The stock solution of the plant extract was prepared in methanol to achieve a concentration of 1 mg/ml.…”

Section: Evaluation Of Dpph Scavenging Activitymentioning

confidence: 99%

In vitrobiological activity of secondary metabolites fromSeseli rigidumWaldst. et Kit. (Apiaceae)

Jakovljević¹,

Vasić²,

Stanković³

et al. 2015

Acta Biologica Hungarica

View full text Add to dashboard Cite

The antioxidant, antimicrobial activity, total phenolic content and flavonoid concentration of Seseli rigidum Waldst. et Kit. were evaluated. Five different extracts of the aboveground plant parts were obtained by extraction with distilled water, methanol, acetone, ethyl acetate and petroleum ether. Total phenols were determined using the Folin-Ciocalteu's reagent, with the highest values obtained in the acetone extract (102.13 mg GAE/g). The concentration of flavonoids, determined by using a spectrophotometric method with aluminum chloride and expressed in terms of rutin equivalent, was also highest in the acetone extracts (291.58 mg RUE/g). The antioxidant activity was determined in vitro using DPPH reagent. The greatest antioxidant activity was expressed in the aqueous extract (46.15 µg/ml). In vitro antimicrobial activities were determined using a microdilution analysis method; minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. Methanolic extract had the greatest influence on bacilli (MIC at 0.0391 mg/ml), but the best antimicrobial effect had acetone and ethyl acetate extracts considering their broad impact on bacteria. According to our research, S. rigidum can be regarded as promising candidate for natural plant source with high value of biological compounds.

show abstract

Screening seeds of some Scottish plants for free radical scavenging activity

Cited by 242 publications

References 26 publications

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

Coumarins from the aerial parts of Prangos uloptera (Apiaceae)

In vitrobiological activity of secondary metabolites fromSeseli rigidumWaldst. et Kit. (Apiaceae)

Contact Info

Product

Resources

About