Screening Resistant Cultivars Against Powdery Mildew, &lt;i&gt;Phytophthora&lt;/i&gt; Rot, and &lt;i&gt;Fusarium&lt;/i&gt; Wilt and Evaluation of Cooking Oil and Egg Yolk Plus and pH adjusted Loess-sulfur Mixture to Control Powdery Mildew

Shim, Chang-Ki; Kim, Yong-Ki; Byeon, Yong-Woong; Park, Jong-Ho; Han, Eun‐Jung; Ko, Byeong-Gu; Kim, Minjeong

doi:10.14397/jals.2018.52.5.31

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Fungicides are conventionally used for managing cucurbit PM infection, but high incidence of fungicide resistance of P. xanthii obstructs disease management and is a concern for human health and the environment [ 15 , 16 , 20 , 21 ]. Therefore, there is an urgent need for developing cucurbits resistant to P. xanthii using other alternative approaches, e.g., the application of epiphytic fungus [ 22 ], bacterial culture by-products [ 23 ], and natural products and oils [ 24 ], or through the breeding of resistant varieties [ 13 , 25 ]. Relatively, molecular breeding is the most accurate, cost-effective, and sustainable approach for breeding pumpkins resistant to P. xanthii compared to the time-consuming and labor-intensive conventional approach.…”

Section: Introductionmentioning

confidence: 99%

GWAS Reveals a Novel Candidate Gene CmoAP2/ERF in Pumpkin (Cucurbita moschata) Involved in Resistance to Powdery Mildew

Alavilli

Lee

You

et al. 2022

IJMS

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Pumpkin (Cucurbita moschata Duchesne ex Poir.) is a multipurpose cash crop rich in antioxidants, minerals, and vitamins; the seeds are also a good source of quality oils. However, pumpkin is susceptible to the fungus Podosphaera xanthii, an obligate biotrophic pathogen, which usually causes powdery mildew (PM) on both sides of the leaves and reduces photosynthesis. The fruits of infected plants are often smaller than usual and unpalatable. This study identified a novel gene that involves PM resistance in pumpkins through a genome-wide association study (GWAS). The allelic variation identified in the CmoCh3G009850 gene encoding for AP2-like ethylene-responsive transcription factor (CmoAP2/ERF) was proven to be involved in PM resistance. Validation of the GWAS data revealed six single nucleotide polymorphism (SNP) variations in the CmoAP2/ERF coding sequence between the resistant (IT 274039 [PMR]) and the susceptible (IT 278592 [PMS]). A polymorphic marker (dCAPS) was developed based on the allelic diversity to differentiate these two haplotypes. Genetic analysis in the segregating population derived from PMS and PMR parents provided evidence for an incomplete dominant gene-mediated PM resistance. Further, the qRT-PCR assay validated the elevated expression of CmoAP2/ERF during PM infection in the PMR compared with PMS. These results highlighted the pivotal role of CmoAP2/ERF in conferring resistance to PM and identifies it as a valuable molecular entity for breeding resistant pumpkin cultivars.

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Section: Introductionmentioning

confidence: 99%

GWAS Reveals a Novel Candidate Gene CmoAP2/ERF in Pumpkin (Cucurbita moschata) Involved in Resistance to Powdery Mildew

Alavilli

Lee

You

et al. 2022

IJMS

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“…호박 재배 중 흰가루병 방제는 일반적으로 살균제에 의존하는데 (Chang et al, 2001;Moon et al, 2010), 오래 전부터 특히 살균제 투입이 많은 포장에서의 저항성 균의 출현이 살균제 사용에 장애가 되고 있다 (O'Brien et al, 1988;O'brien, 1994;Lebeda et al, 2010;Vielba-Fernández et al, 2018). 따라서 살균제 이외에 자연 생산물과 오일 (Shim et al, 2018), 길항기작 을 갖는 세균과 진균(bio-agent) (Gafni et al, 2015;Hafez et al, 2018), 세균배양 부산물 (Tanaka et al, 2017)을 사용하는 등 의 다양한 생물적 방제가 시도되고 있다. 그러나 저항성 자원을 탐색하여 이용하는 유전•육종적 방법이 효과가 지속적이고 경 제적이므로 가장 각광을 받고 있다 (Cho et al, 2004;Cho et al, 2005;Kim et al, 2014;Holdsworth et al, 2016;Guo et al, 2018;Guo et al, 2019).…”

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QTL Mapping and molecular markers of Powdery Mildew Resistance in Pumpkin (Cucurbita moschata)

Park¹,

Jang²,

Yu³

et al. 2020

HST

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Cucurbita moschata, an economically and nutritionally important vegetable in Korea, is seriously damaged in fruit quality and productivity by powdery mildew fungus (Podosphaera xanthii). To understand genetic bases of the powdery mildew disease resistance, an F 2 population was developed from a cross between TG201 (C. moschata, susceptible, female parent) and TG10 (resistant, male parent) heirloom from Hongikbio Inc. Randomly selected 204 F2 individuals were assayed against powdery mildew pathogen, and construction of genetic map and QTL analysis were conducted using SNPs derived from genotyping-by-sequencing (GBS) method. The single major QTL was located in 6.9 -7.3 Mb region of TG10 chromosome 3. Within the 400 kb region, we investigated DNA insertion and deletion (indel) variations between TG201 and TG10 genome, and successfully designed seven sets of indel PCR markers. The functional validity of the powdery mildew resistance markers was confirmed with F2 individuals, breeding lines, and commercial varieties. From genomic sequence comparison on the basis of the breeding history, we also could clarify that the powdery mildew resistance region was introduced from C. okeechobeensis subsp. martinezii to C. moschata. Moreover, whole genome assemblies of two heirloom parents and genome-wide SNP/indel information were produced and are expected to be utilized as a genome resource for study of genus Cucurbita. The results and information of this study will be useful for genetics researches and breeding practices of C. moschata.

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Research Status of Environmental Friendly Management for Korean Melon Powdery Mildew

Yeon¹,

Shim²,

Kim³

et al. 2020

kjps

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Screening Resistant Cultivars Against Powdery Mildew, <i>Phytophthora</i> Rot, and <i>Fusarium</i> Wilt and Evaluation of Cooking Oil and Egg Yolk Plus and pH adjusted Loess-sulfur Mixture to Control Powdery Mildew

Cited by 3 publications

References 5 publications

GWAS Reveals a Novel Candidate Gene CmoAP2/ERF in Pumpkin (Cucurbita moschata) Involved in Resistance to Powdery Mildew

GWAS Reveals a Novel Candidate Gene CmoAP2/ERF in Pumpkin (Cucurbita moschata) Involved in Resistance to Powdery Mildew

QTL Mapping and molecular markers of Powdery Mildew Resistance in Pumpkin (Cucurbita moschata)

Research Status of Environmental Friendly Management for Korean Melon Powdery Mildew

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