Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8

Tuo, Yanfeng; Xing, Song; Song, Yinglong; Liu, Wenwen; Tang, Yu; Gao, Yuan; Jiang, Shujuan; Qian, Fang; Mu, Guangqing

doi:10.3168/jds.2017-13654

Cited by 49 publications

(31 citation statements)

References 27 publications

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“…1). This property is strain specific and it is usually screened in vitro using cell lines infected with pathogens (Walsham et al 2016; Jessie Lau and Chye 2018; Tuo et al 2018). For instance, several clinical trials have demonstrated the effectiveness of Lactobacillus rhamnosus LGG against vancomycin-resistant enterococci in colonized patients (Szachta et al 2011; Manley et al 2007).…”

Section: Antagonism Against Pathogensmentioning

confidence: 99%

Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health

Monteagudo-Mera

Rastall

Gibson

et al. 2019

Appl Microbiol Biotechnol

423

238

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Adhesion ability to the host is a classical selection criterion for potential probiotic bacteria that could result in a transient colonisation that would help to promote immunomodulatory effects, as well as stimulate gut barrier and metabolic functions. In addition, probiotic bacteria have a potential protective role against enteropathogens through different mechanisms including production of antimicrobial compounds, reduction of pathogenic bacterial adhesion and competition for host cell binding sites. The competitive exclusion by probiotic bacteria has a beneficial effect not only on the gut but also in the urogenital tract and oral cavity. On the other hand, prebiotics may also act as barriers to pathogens and toxins by preventing their adhesion to epithelial receptors. In vitro studies with different intestinal cell lines have been widely used along the last decades to assess the adherence ability of probiotic bacteria and pathogen antagonism. However, extrapolation of these results to in vivo conditions still remains unclear, leading to the need of optimisation of more complex in vitro approaches that include interaction with the resident microbiota to address the current limitations. The aim of this mini review is to provide a comprehensive overview on the potential effect of the adhesive properties of probiotics and prebiotics on the host by focusing on the most recent findings related with adhesion and immunomodulatory and antipathogenic effect on human health.

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Section: Antagonism Against Pathogensmentioning

confidence: 99%

Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health

Monteagudo-Mera

Rastall

Gibson

et al. 2019

Appl Microbiol Biotechnol

423

238

View full text Add to dashboard Cite

show abstract

“…These beneficial benefits may include preventing diarrhea ( Staudacher, 2015 ; Sinclair et al, 2016 ; Kim et al, 2018 ), modulating the intestinal community ( Marteau et al, 2002 ; Xie et al, 2016 ), improving lactose tolerance ( Mustapha et al, 1997 ; Staudacher, 2015 ; Oak and Jha, 2018 ), reducing the serum cholesterol level ( Choi et al, 2015 ; Kobyliak et al, 2016 ), suppressing pathogens ( Kim et al, 2014 ; Tuo et al, 2018 ), and boosting the immune system ( Dimitrijevic et al, 2014 ; Kemgang et al, 2014 ). To maximize these functions, Lactobacillus strains must be able to adhere to and colonize the gastro-intestinal tract ( Ljungh and Wadstrom, 2006 ; Tuo et al, 2018 ). Therefore, one important criterion in selecting probiotic bacteria is their ability to adhere to epithelial cells of the intestinal tract.…”

Section: Introductionmentioning

confidence: 99%

A Surface Protein From Lactobacillus plantarum Increases the Adhesion of Lactobacillus Strains to Human Epithelial Cells

et al. 2018

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Adhesion to epithelial cells is considered important for Lactobacillus to exert probiotic effects. In this study, we found that trypsin treatment decreased the adhesion ability of Lactobacillus plantarum AR326 and AR269, which exhibit good adhesion ability, and surface proteins extracts increased the adhesion of the strains with poor adhesion ability. By SDS–polyacrylamide gel electrophoresis and mass spectrometry analysis, the main component of the surface proteins was detected and identified as a protein of approximately 37 kDa. It was 100% homologous with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from L. plantarum WCFS1. The adhesion of AR326 and AR269 was decreased significantly by blocking with the anti-GAPDH antibody, and GAPDH restored the adhesion of AR326 and AR269 treated with trypsin. In addition, purified GAPDH significantly increased the adhesion of the strains with poor adhesion ability. These results indicated that GAPDH mediates the adhesion of these highly adhesive lactobacilli to epithelial cells and can be used to improve the adhesion ability of probiotics or other bacteria of interest.

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“…L. casei significantly inhibits the growth of pathogenic bacteria as well. A recent study showed that 11 Lactobacillus strains can inhibit adhesion of E. coli to Caco‐2 cells, with L. plantarum and LGG exhibiting higher inhibition rates (Tuo et al, 2018). Studies have shown that L. acidophilus LA1 inhibits the adhesion of pathogenic E. coli , Salmonella typhimurium and Yersinia pseudotuberculosis to cells (Bernet et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Anti‐adhesive effects of sialic acid and Lactobacillus plantarum on Staphylococcus aureus in vitro

Yang

Liu

et al. 2020

Journal of Food Safety

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Staphylococcus aureus (S. aureus) is a common food‐borne pathogen that causes severe diseases after adhesion to epithelial cells. Lactobacillus inhibits pathogenic bacterial adhesion and infection. In addition, sialic acid (SA) is widely known for its beneficial biological functions. A new way of reducing the occurrence of diseases and curbing the overuse of antibiotics is ingesting prebiotics and probiotics that regulate the intestinal flora. In this study, we first evaluated the anti‐adhesive effects of several strains of Lactobacillus on S. aureus. The study revealed that the S. aureus adhesion was inhibited by all the strains of Lactobacillus. Besides, the rate of inhibition by L. plantarum Z‐4 was significantly higher than other Lactobacillus species. We then investigated the effects of different SA concentrations (40, 100, 150, 200, and 260 μg/ml) on the growth and adhesion characteristics of L. plantarum and S. aureus. The results showed that SA influences bacterial adhesion by regulating the bacteria's growth characteristics. Finally, the effects of SA combined with Lactobacillus on the adhesion of S. aureus were assessed by competition, exclusion and displacement methods. SA with a concentration of 260 μg/mL combined with L. plantarum had the highest inhibition effect on the competition assays. In addition, the expression of S. aureus adhesion‐related genes was reduced. This provides a new perspective on the application of SA and/or L. plantarum and its potential to resist adhesion of S. aureus.

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Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8

Cited by 49 publications

References 27 publications

Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health

Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health

A Surface Protein From Lactobacillus plantarum Increases the Adhesion of Lactobacillus Strains to Human Epithelial Cells

Anti‐adhesive effects of sialic acid and Lactobacillus plantarum on Staphylococcus aureus in vitro

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