Screening Potential Probiotic Characteristics of<i> Lactobacillus brevis</i> Strains In Vitro and Intervention Effect on Type I Diabetes In Vivo

Abdelazez, Amro; Abdelmotaal, Heba; Evivie, Smith Etareri; Melak, Sherif; Jia, Fang-Fang; Khoso, Mir Hassan; Zhu, Zong-Tao; Zhang, Lu-Ji; Sami, Rokayya; Meng, Xiang-Chen

doi:10.1155/2018/7356173

Cited by 53 publications

(35 citation statements)

References 63 publications

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“…It has been an everlasting struggle for humans to keep food safe and wholesome (Papadimitrou et al, 2015). Functional food acts as beneficial compounds or foods containing microorganisms exhibiting a pivotal role in strengthening and enriching health well-being and suppressing some strict disease, for instance, obesity, diabetes, atherosclerosis, heart disease, retinopathy, kidney toxicity, atherosclerosis, hypertension, diabetic foot ulcers, and cystic fibrosis (Ji et al, 2015; Abdelazez et al, 2017). Many lactic acid bacteria (LAB) strains serve as probiotics in functional foods.…”

Section: Introductionmentioning

confidence: 99%

In vitro Organic Acid Production and In Vivo Food Pathogen Suppression by Probiotic S. thermophilus and L. bulgaricus

Evivie

Abdelazez

et al. 2019

Front. Microbiol.

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Foodborne pathogens are a major source of morbidity and mortality worldwide. For this cause, exploring various effective ways of suppressing their spread is at the forefront of many research projects. The current study aims to investigate the in vitro organic acid production of S. thermophilus KLDS 3.1003 and L. bulgaricus KLDS 1.0207 strains, their in vivo suppression of and immuno-modulatory effects against E. coli ATCC 25922 and S. aureus ATCC 25923 pathogens. First, lactic and acetic acid production using three carbon sources – 1% glucose (control), 1% sucrose, and 1% fructo-oligosaccharides (FOS) – was determined by HPLC. For the in vivo section, a total of 40 BALB/c mice were purchased and divided into 10 treatment groups (control and nine treatments). Animals were given 1 week to acclimatize and then fed treatment diets for 14 days. Afterward, hematological (RBC, WBC, HB, PLT, Neutrophils, Eosinophils, Lymphocytes, and Monocytes) and histopathological analyses were carried out. All analyses were done in triplicate. Results show that lactic and acetic acid productions for both strains increased with supplementation and were highest after 1% FOS addition. Regardless of carbon source, L. bulgaricus KLDS 1.0207 produced higher ( P < 0.05) amounts of lactic and acetic acids than S. thermophilus KLDS 3.1003. Also, generally better hematological parameters in probiotic groups than the control ( P < 0.05) were observed. In some instances, mice in probiotic treatment groups had better immunity levels (lymphocytes, monocytes, neutrophils, eosinophils) than those in the control and pathogen groups. Histopathological studies showed that no anomalies were associated with S. thermophilus KLDS 3.1003 and L. bulgaricus KLDS 1.0207 administration. In conclusion, S. thermophilus KLDS 3.1003 and L. bulgaricus KLDS 1.0207 strains are not only probiotic candidates but can have therapeutic applications.

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Section: Introductionmentioning

confidence: 99%

In vitro Organic Acid Production and In Vivo Food Pathogen Suppression by Probiotic S. thermophilus and L. bulgaricus

Evivie

Abdelazez

et al. 2019

Front. Microbiol.

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“…The main SCFAs that possess neuroactive properties are acetate, butyrate, and propionate [63][64][65][66][67][68][69]. Acetate and propionate are mainly produced by Bacteroidetes, whereas Firmicutes contribute primary to the production of butyrate [42,44]. Acetate is the most abundant metabolite in the colon and stool flowed by propionate and butyrate [45].…”

Section: The Gut-brain Axismentioning

confidence: 99%

“…According to the Food and Drug Administration (FDA) and WHO, probiotics are defined as "live micro-organisms which can provide health benefits on the host when administered in adequate amounts" [42,43]. Probiotics may be added individually or in a mixture of different strains that work in synergy and have additive benefits [44].…”

Section: Introductionmentioning

confidence: 99%

The Promising Role of Probiotics in Managing the Altered Gut in Autism Spectrum Disorders

Abdellatif

McVeigh

Bendriss

et al. 2020

IJMS

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Gastrointestinal symptoms (GIS) have been reported repeatedly in people with autism spectrum disorder (ASD) and studies have reported interesting correlations between severity of behavioral and gastrointestinal symptoms. Growing evidence indicates that the gut microbiota in ASD is altered with various shifts described at different taxonomic levels, pointing to the importance of considering the gut–brain axis in treatment of these disorders. Probiotics are live beneficial bacteria that are ingested as food or customized pills. These beneficial bacteria, when added in sufficient amounts, can correct the dysbiosis. Because probiotics have shown success in treating irritable bowel syndrome (IBS), it is plausible to investigate whether they can induce alleviation of behavioral symptoms as well. Probiotics show, in some clinical studies, their potential benefits (1) in improving gastrointestinal dysfunction, (2) in correcting dysbiosis, (3) in consequently reducing the severity of ASD symptoms. This review compiles data from selected studies that investigate these benefits and the mechanisms that mediate these effects, which include the production of metabolites, hormones, and neurotransmitters and the regulation of pro-inflammatory and regulatory cytokines. Future research based on more randomized, controlled studies with a larger population size and standardized use of strains, concentration of probiotics, duration of treatments, and methods of DNA extraction is still needed in this area, which may lead to more robust results.

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“…The elucidation of differences in the mechanisms of action of T1D in humans and animals will facilitate experimental research and aid in discovering novel therapeutic strategies to ameliorate the T1D. Some of the most common animal models used to study the effect of probiotics, prebiotics and drugs on T1D are NOD mice [61], Streptozotocin (STZ)-induced T1D rats and mice [62,63], Alloxan-induced T1D Swiss Webster mice [64], BBDR rats [65,66], and Bio-Breeding Diabetic Pathogen (BBDP) rats [65,66]. The interactions of probiotics and prebiotics with gut microbiota and immune system and their involvement in T1D pathology are discussed separately for human and animal models.…”

Section: Role Of the Gut Microbiota-immune Axis In T1dmentioning

confidence: 99%

“…In a study on C57BL/6 mice, the feeding of specific probiotic Lactobacillus (L.) brevis strains ( L. brevis KLDS 1.0727 and L. brevis KLDS 1.0373) has been found to protect against STZ-induced T1D and reduce blood glucose levels via gamma-aminobutyric acid (GABA) [62]. In another study on STZ-induced diabetic mice, strains belonging to Bifidobacterium (B.)…”

Section: Probiotic Interventions To Ameliorate T1dmentioning

confidence: 99%

Probiotics and Prebiotics for the Amelioration of Type 1 Diabetes: Present and Future Perspectives

et al. 2019

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Type 1-diabetes (T1D) is an autoimmune disease characterized by immune-mediated destruction of pancreatic beta (β)-cells. Genetic and environmental interactions play an important role in immune system malfunction by priming an aggressive adaptive immune response against β-cells. The microbes inhabiting the human intestine closely interact with the enteric mucosal immune system. Gut microbiota colonization and immune system maturation occur in parallel during early years of life; hence, perturbations in the gut microbiota can impair the functions of immune cells and vice-versa. Abnormal gut microbiota perturbations (dysbiosis) are often detected in T1D subjects, particularly those diagnosed as multiple-autoantibody-positive as a result of an aggressive and adverse immunoresponse. The pathogenesis of T1D involves activation of self-reactive T-cells, resulting in the destruction of β-cells by CD8+ T-lymphocytes. It is also becoming clear that gut microbes interact closely with T-cells. The amelioration of gut dysbiosis using specific probiotics and prebiotics has been found to be associated with decline in the autoimmune response (with diminished inflammation) and gut integrity (through increased expression of tight-junction proteins in the intestinal epithelium). This review discusses the potential interactions between gut microbiota and immune mechanisms that are involved in the progression of T1D and contemplates the potential effects and prospects of gut microbiota modulators, including probiotic and prebiotic interventions, in the amelioration of T1D pathology, in both human and animal models.

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Screening Potential Probiotic Characteristics of Lactobacillus brevis Strains In Vitro and Intervention Effect on Type I Diabetes In Vivo

Cited by 53 publications

References 63 publications

In vitro Organic Acid Production and In Vivo Food Pathogen Suppression by Probiotic S. thermophilus and L. bulgaricus

In vitro Organic Acid Production and In Vivo Food Pathogen Suppression by Probiotic S. thermophilus and L. bulgaricus

The Promising Role of Probiotics in Managing the Altered Gut in Autism Spectrum Disorders

Probiotics and Prebiotics for the Amelioration of Type 1 Diabetes: Present and Future Perspectives

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