Screening of UV-absorbing solutes in uremic serum by reversed phase HPLC — Change of blood levels in different therapies

Schoots, A.C.; Homan, H.R.; Gladdines, MM Marcel; Cramers, C.A.M.G.; Smet, Rita De; Ringoir, S

doi:10.1016/0009-8981(85)90122-6

Cited by 42 publications

(27 citation statements)

References 12 publications

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“…However, protein binding and compartmentalization may influ ence solute removal during hemodialysis. In an earlier publication, concentration changes of some UV-absorbing solutes during hemodialysis and hémodiafiltration were reported [6], However, in that study membranes and blood and dialysate flow rates were not uniform among the patients. The present study describes the effect of hemodialysis treatment on blood levels of low molecular mass uremic solutes.…”

Section: Introductionmentioning

confidence: 74%

“…Total concentrations of protein-bound solutes (e.g. free + bound fraction) were determined in serum samples after precipitation of the protein by trichloroacetic acid (TCA) as described elsewhere [7], HPLC Analysis HPLC analyses were performed as described earlier [6,7], An Ultrasphere Octyl (C8-modified silica) column (4.6 mm in diame ter x 25 cm), with 5-um particles, was used in conjunction with an Ultrasphere Octyl guard column (2mm in d iam eterx3 cm), with 10-pm particles.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The present study describes the effect of hemodialysis treatment on blood levels of low molecular mass uremic solutes. We have used high-pressure liquid chromatography (HPLC) [6][7][8] to analyze serum concen trations of protein-bound and nonbound solutes. Protein binding levels were determined in pre-and postdialysis serum samples in order to estimate the influence on dialyzer clearance, which is one of the factors determin ing (weekly averaged) serum concentrations of accumu lating solutes in uremic patients.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Hemodialysis on Serum Concentrations of HPLC-Analyzed Accumulating Solutes in Uremia

Schoots

Peeters

Gerlag³

1989

Nephron

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The concentration changes, during hemodialysis treatment, of 18 characteristic uremia compounds, analyzed by high-pressure liquid chromatography in sera of renal patients were studied. Pre- to postdialysis concentration ratios (dialysis ratio, D) varied from 0.83 to 3.04 for the different solutes. A division into three solute groups, on the basis of their D values, could be rationalized qualitatively from data on protein binding and dialyzer clearance. One group showed low dialysis ratios which could be explained from plasma protein binding. The second group had intermediate D values, comparable to those of creatinine. For some of the members of the third group, high D values might indicate a compartmentalization and resistance to mass transfer across biological membranes. Among the latter are the tubularly secreted hippuric acid and p-hydroxyhippuric acid. For most of the (protein-bound) solutes, protein binding was shown to decrease during hemodialysis. Protein binding levels were higher after dialysis only for hippuric acid and the as yet unidentified fluorescent solute designated UFK8. In conclusion, the change of serum concentrations and of protein binding resulting from hemodialysis treatment are presented and are compared for 18 accumulating solutes in sera of patients with end-stage renal failure.

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Section: Introductionmentioning

confidence: 74%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Hemodialysis on Serum Concentrations of HPLC-Analyzed Accumulating Solutes in Uremia

Schoots

Peeters

Gerlag³

1989

Nephron

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“…However, in contrast with other nucleosides, it is not reincorporated into other molecules of tRNA after the breakdown of parent tRNA molecule but excreted by the kidney as an endproduct [14]. tp elimination is complex: glomerular filtration, resorption [18][19][20] in the proximal tubule and secretion in the distal nephron [20,21]. Its net excretion but also resorption increases in residual nephrons of RF patients with its marked accumulation in RF [14,22], Glucose Utilization and Insulin Resistance in Striated Muscle t)/ inhibits both basal and insulin-stimulated glucose utilization.…”

Section: Magnesium Depletionmentioning

confidence: 99%

Inhibition of Glucose Utilization in Isolated Rat Soleus Muscle by Pseudouridine: Implications for Renal Failure

Dzúrik¹,

Spustová²,

Lajdova³

1993

Nephron

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Pseudouridine (ψ) is an outstanding nucleoside which is not rebuilt into the tRNA once the parent tRNA is broken down, ψ inhibits basal glucose utilization in isolated rat soleus muscle with intact membrane with AD₅₀ 42 μmol/l and C_max 66%. ψ at concentrations found in renal failure patients inhibits both the insulin-and tolbutamide-stimulated glucose utilization. Its inhibitory activity is partially additive with diltiazem inhibition and nonadditive in the case of magnesium depletion. It is concluded that ψ inhibits glucose utilization at the level of Ca modulation in the insulin regulatory cascade.

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“…Some naturally fluorescent compounds have been separated and identified with a fluorescence detector, in both serum and haemodialysate (Niwa, 1993;Barnett, 1985;Swan, 1983). A liquid chromatographic method including a UV detector for detection of UV-absorbing solutes in uremic serum has been developed (Schoots, 1985;Senftleber, 1976;Knudson 1978). A fixed wavelength at 254 nm was mainly used in these studies.…”

Section: Hplc As An Analysis Tool For Biological Samplesmentioning

confidence: 99%

Optical Dialysis Adequacy Monitoring: Small Uremic Toxins and Contribution to UV-Absorbance Studied by HPLC

Lauri¹,

Arund²,

Holmar³

et al. 2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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Screening of UV-absorbing solutes in uremic serum by reversed phase HPLC — Change of blood levels in different therapies

Cited by 42 publications

References 12 publications

Effect of Hemodialysis on Serum Concentrations of HPLC-Analyzed Accumulating Solutes in Uremia

Effect of Hemodialysis on Serum Concentrations of HPLC-Analyzed Accumulating Solutes in Uremia

Inhibition of Glucose Utilization in Isolated Rat Soleus Muscle by Pseudouridine: Implications for Renal Failure

Optical Dialysis Adequacy Monitoring: Small Uremic Toxins and Contribution to UV-Absorbance Studied by HPLC

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