Screening of the potential α-amylase inhibitor in essential oil from Cedrus deodara cones

Xu, Fa; Gu, Dongyu; Wang, Miao; Zhu, Lin; Chu, Tingting; Cui, Yuna; Tian, Jing; Wang, Yi; Yang, Yi

doi:10.1016/j.indcrop.2017.04.006

Cited by 26 publications

(12 citation statements)

References 30 publications

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“…Considering the importance of edible oil flavor, many studies have identified the volatile compounds present in oils using gas chromatography–mass spectrometry (GC–MS). [ 15–17 ] Pacioni et al. [ 18 ] performed a blind analysis of 18 olive oil samples flavored with white and black truffles from the Italian market using GC–MS and an electronic nose.…”

Section: Introductionmentioning

confidence: 99%

“…Considering the importance of edible oil flavor, many studies have identified the volatile compounds present in oils using gas chromatography-mass spectrometry (GC-MS). [15][16][17] Pacioni et al [18] performed a blind analysis of 18 olive oil samples flavored with white and black truffles from the Italian market using GC-MS and an electronic nose. Madhumita et al [19] detected the major compounds, including eugenol, estragole, linalool, -copaene, anethole, chavicol, and caryophyllene in betel leaf essential oil using GC-MS. Zhang et al [20] analyzed the change in volatile compounds in tuna oil during storage using GC-MS with laser irradiation.…”

Section: Introductionmentioning

confidence: 99%

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Fatty Acids and Flavor Components in the Oil Extracted from Golden Melon Seeds

Chen

Zhu

et al. 2021

Euro J Lipid Sci & Tech

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For complete utilization of raw materials and edible oil production, the oil from golden melon seeds (GSs) is extracted via the cold‐pressing, hot‐pressing, and ultrasound‐assisted aqueous enzymatic extraction (AEE), and the fatty acid composition, and nutritional value of the extracted GS oils (GSOs) are analyzed. The volatile compounds present in the GSOs are determined using gas chromatography–mass spectrometry. The aroma profiles of different GSO samples are further distinguished by using an electronic nose. A total of 16 fatty acids are identified in the GSO samples, with atherogenic, thrombogenic, and nutritive value indices ranging from 0.142–0.151, 0.366–0.403, and 5.019–5.299, respectively. Moreover, 43 volatile compounds, including esters, hydrocarbons, alcohols, ketones, pyrazines, and aldehydes are identified. The cold‐pressed GSO presents fresh and fruity flavors, while the hot‐pressed GSO presents roasted, nutty, fatty, and fruity flavors, and the GSO obtained via AEE presents fatty and fruity flavors. The acid and peroxide contents of these oil samples are 0.69–079 mg g−1 and 5.17–5.79 mmol kg−1, respectively. The results indicate that the extraction method affects the fatty acid composition, flavor components, and physiochemical properties of the GSO. This study may help promote the development of edible GSO. Practical applications: With high demand for edible oils, it is of great significance to find natural edible oils from different sources. The oil in golden melon seeds is extracted by different methods. Their fatty acid composition and flavor components are analyzed. The GSO extracted via AEE method is rich in fatty acids composition. This work would guide the development of an edible GSO and increase the value of golden melon.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fatty Acids and Flavor Components in the Oil Extracted from Golden Melon Seeds

Chen

Zhu

et al. 2021

Euro J Lipid Sci & Tech

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“…In response to insulin, Glut-4 expression increases and the translocation of Glut-4 containing vesicles to the plasma membrane is induced, resulting in glucose uptake into the cell [ 46 ]. In T2DM, the expression of Glut-4 , which is insufficiently stimulated due to insulin resistance, decreases, its localization is impaired and glucose transport in target tissues is prevented [ 3 , 47 ]. Since insulin resistance and decreased Glut-4 levels in adipocyte tissue are characteristic, the development of insulin-sensitizing agents against T2DM is an important current research topic [ 26 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes

et al. 2021

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Diabetes mellitus (DM) is a metabolic disorder with chronic hyperglycemia featured by metabolic outcomes owing to insufficient insulin secretion and/or insulin effect defect. It is critical to investigate new therapeutic approaches for T2DM and alternative, natural agents that target molecules in potential signal pathways. Medicinal plants are significant resources in the research of alternative new drug active ingredients. Bolanthus spergulifolius (B. spergulifolius) is one of the genera of the family Caryophyllaceae. In this study, it was explored the potential anti-diabetic effects in vitro of B. spergulifolius extracts on 3T3-L1 adipocytes. The total phenolic contents (TPC) of methanolic (MeOH), ethyl acettate (EA) and aqueous extracts of B. spergulifolius were evaluated via Folin-Ciocateau. B. spergulifolius extracts showing highly TPC (Aqueous< MeOH< EA) and their different concentrations were carried out on preadipocytes differentiated in to mature 3T3-L1 adipocytes to investigate their half-maximal (50%) inhibitory concentration (IC50) value by using Thiazolyl blue tetrazolium bromide (MTT) assay. The IC50 of MeOH, EA and Aqueous extracts were observed as 305.7 ± 5.583 μg/mL, 567.4 ± 3.008 μg/mL, and 418.3 ± 4.390 μg/mL and used for further experiments. A live/dead assay further confirmed the cytotoxic effects of MeOH, EA and Aqueous extracts (respectively, 69.75 ± 1.70%, 61.75 ± 1.70%, 70 ± 4.24%, and for all p< 0.05). Also, effects of extracts on lipid accumulation in mature 3T3-L1 adipocytes were evaluated by Oil-Red O staining assay. The extracts effectively decreased lipid-accumulation compared to untreated adipocytes (for all p< 0.05). Moreover, effect of extracts on apoptosis regulated by the Bax and Bcl-2 was investigated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The extracts significantly induced apoptosis by up-regulating pro-apoptotic Bax expression but down-regulated anti-apoptotic Bcl-2 gene expression compared to untreated adipocytes (for all p< 0.05). The Glut-4 expression linked with insulin resistance was determined by qRT-PCR, Western-blot analysis, and immunofluorescence staining. In parallel, the expression of Glut-4 in adipocytes treated with extracts was significantly higher compared to untreated adipocytes (for all p< 0.05). Extracts significantly suppressed cell migration after 30 h of wounding in a scratch-assay (for all p< 0.05). Cell morphology and diameter were further evaluated by phase-contrast microscopy, scanning electron microscopy, Immunofluorescence with F-Actin and Giemsa staining. The adipocytes treated with extracts partially lost spherical morphology and showed smaller cell-diameter compared to untreated adipocytes (for all p< 0.05). In conclusion, these results suggest that extracts of B. spergulifolius cause to an induce apoptosis, decrease lipid-accumulation, wound healing, up-regulating Glut-4 level and might contribute to reducing of insulin-resistance in DM.

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“…Longipinene extracted from the EOs of cones of C. deodara showed an α‐amylase inhibitory effect with a maximum inhibition rate of 88.03% at 600 μg/ml concentration (Xu et al, 2017). Pine ( Pinus koraiensis ) EO reported for hypoglycemic potential as it significantly suppressed the ‐amylase activity secreted from the pancreas, inhibit endothelial NO synthase (eNOS), reactive oxygen species (ROS), and blood glucose level in a concentration‐dependent manner which improved the insulin expression and vascular endothelial growth factor (VEGF) in streptozotocin (STZ) treated mice and HIT‐T15 pancreatic cells as a potent antidiabetic agent (Joo et al, 2013).…”

Section: Pharmacological Activities Of Conifers From Northwestern Himmentioning

confidence: 99%

Review on essential oils, chemical composition, extraction, and utilization of some conifers in Northwestern Himalayas

Bhardwaj

Islam

Jayasena

et al. 2020

Phytotherapy Research

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Essential oils (EOs) are regarded as alternative therapeutic agents for many diseases. In phytotherapy research areas, it is now well reported that conifers are the rich source of EOs. This review aims to update information on the biological sources and the best extraction processes of the significant constituents along with the traditional and therapeutic properties of the EOs from selected conifers of Himachal Pradesh, Northwestern Himalaya. In the present review, ten conifer species of high values have been selected. Results from several studies suggest that the conifers contain monoterpenes, sesquiterpenes, diterpenes, ketones, alcohols, and esters, which are used in medicines, food products, and cosmetics as well as other commercial and industrial products. Traditionally, the EOs from the conifers have been reported to be used against fever, cough, bronchitis, skin diseases, gastrointestinal disorders, and asthma. The pharmacological studies suggest that these EOs can be used as antirheumatic, antiseptic, antispasmodic, anticancer, anti-inflammatory, antitoxic, aphrodisiac, and astringent agents. It is, therefore, concluded that the EOs from the conifers might be one of the promising tools for the treatment of various diseases. Extensive research is required to ascertain the efficacy of the EOs from unstudied conifers.

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Screening of the potential α-amylase inhibitor in essential oil from Cedrus deodara cones

Cited by 26 publications

References 30 publications

Fatty Acids and Flavor Components in the Oil Extracted from Golden Melon Seeds

Fatty Acids and Flavor Components in the Oil Extracted from Golden Melon Seeds

Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes

Review on essential oils, chemical composition, extraction, and utilization of some conifers in Northwestern Himalayas

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