Screening of the essential oil effects on human H1N1 influenza virus infection: an <i>in vitro</i> study in MDCK cells

Najar, Basma; Nardi, Valeria; Stincarelli, Maria Alfreda; Patrissi, Samuele; Pistelli, Luisa; Giannecchini, Simone

doi:10.1080/14786419.2021.1944137

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…o-Cymene is one of the major the main components (65.2%) of the essential oils from Bursera simaruba (L.) Sarg., Thymus vulgaris ((56.2%) and Nigella sativa L. seeds (37.82%). The latter essential oil exhibits anti-oxidative properties, anti-microbial effect against clinically significant strains of bacteria and fungi whereas the thyme oil possesses potential activity against fungi causing the brown rot disease, what again brings the perspective of use of in vitro pitaya cultures industrial importance, however it has to be highlighted that for this purpose further optimalization of the process would be required due to low share of this compound in the present study Besides, o-cymene shows anti-viral effect against the virus of human influenza H1N1 [63][64][65][66][67].…”

Section: Discussionmentioning

confidence: 83%

The Potential Use of Plant Growth Regulators for Modification of the Industrially Valuable Volatile Compounds Synthesis in Hylocreus undatus Stems

et al. 2023

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The pitaya (dragon fruit) Hylocereus is a genus which belongs to the Cactaceae family. It is native to Mexico, occurring also in other regions of Central and South America. Pitaya fruit is mainly intended for consumption and for this reason the species is grown commercially. The fruit is a rich source of vitamins, biologically active compounds, and dietary fibre. Using in vitro culture can accelerate the process of reproduction and growth of pitaya plants. Profiling of volatile compounds contained in the stem of Hylocereus undatus was carried out using the SPME-GC-MS technique. The main compounds present were hexanal, 2-hexenal and 1-hexanol. The results showed differences in the occurrence of volatile compounds between plants grown in media with an addition of BA (6-benzylaminopurine) and IAA (indole-3-acetic acid), which have been used as plant growth regulators. Statistically significant differences between the contents of volatile compounds were observed in the case of 2-hexenal and 1-hexanol. The effect of BA on reducing the amount of volatile compounds was observed. However, introduction of IAA to the in vitro medium resulted in more compounds being synthesized. This study is the first to describe the volatile compounds in the pitaya stem. The results indicate that plant hormones are able to modify the profile of volatile compounds.

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Section: Discussionmentioning

confidence: 83%

The Potential Use of Plant Growth Regulators for Modification of the Industrially Valuable Volatile Compounds Synthesis in Hylocreus undatus Stems

et al. 2023

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“…Cymene isomers are reported to possess insecticidal and repellent properties [41]. Also, the recent studies con rmed that ocymene has an inhibitory effect against the H1N1 in uenza virus [42] and coronavirus 2 [43]. The study assumes that the maximum existence same molecular weight derivative of dipentene, and γ-terpinene in black pepper was the reason for antioxidant activity [44].…”

Section: Biochemical Analysismentioning

confidence: 97%

Antibacterial Activity of Ethyl Acetate Extract of Endophytic Fungus (Paraconiothyrium Brasiliense) Through Targeting Pathogenesis Related Dihydropteroate Synthase (DHPS)

Sathiyaseelan

Saravanakumar

Anand

et al. 2021

Preprint

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Endophytic fungi are innumerable sources of bioactive metabolites with various biomedical applications. Hence, this study was aimed to isolate and identify the endophytic fungus Paraconiothyrium brasiliense (PB) from the fruit of Capsicum annuum and evaluated their biochemical, antimicrobial, antioxidant, cell viability, and cytotoxicity behavior. Results showed that ethyl acetate extract (EAE) of PE contains flavonoid (31.53 ± 0.9 mg of QE/g of extract) and phenolic (2.59 ± 0.06 mg of GAE/g of extract). The PB-EAE showed significant antioxidant activity in terms of free radicals such as DPPH, ABTS, and Fe3+ scavenging. The antioxidant potential of PB-EAE reflects in normal cell viability and anticancer activity against prostate cancer (PC3) cells. The bacterial inhibitory activity of PB-EAE was equal to commercial antibiotic tetracycline hydrochloride (TCH). Further, GC-MS analysis confirmed the presence of volatile molecules such as o-cymene (CH3C6H4CH(CH3)2), dipentene (C10H16), γ-terpinene (C10H16), 4-carvomenthenol (C10H18O), heneicosane (CH3(CH2)19CH3), 2,5-piperazinedione (C4H6N2O2). Also, molecular docking analysis demonstrated the significant interactions between the compounds of PB-EAE and Dihydropteroate Synthase (Protein ID:5JQ9; DHPS). This study revealed that endophytic P. brasiliense from Capsicum annuum could be a novel source for the isolation of antimicrobials.

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“…Several studies have reported the antiviral activity of EOs against influenza A virus (IAV) [18][19][20][21][22][23][24][25][26], even though these works were carried out with the liquid phases of the oils and their components. On the other hand, it was demonstrated that the vapor phase of several EOs showed good antibacterial, antifungal and antiviral activity, sometimes better than the corresponding liquid phase of the oil [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Damages to H1N1 Influenza Virus Caused by Vapor Essential Oils

et al. 2022

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Influenza viruses are transmitted from human to human via airborne droplets and can be transferred through contaminated environmental surfaces. Some works have demonstrated the efficacy of essential oils (EOs) as antimicrobial and antiviral agents, but most of them examined the liquid phases, which are generally toxic for oral applications. In our study, we describe the antiviral activity of Citrus bergamia, Melaleuca alternifolia, Illicium verum and Eucalyptus globulus vapor EOs against influenza virus type A. In the vapor phase, C. bergamia and M. alternifolia strongly reduced viral cytopathic effect without exerting any cytotoxicity. The E. globulus vapor EO reduced viral infection by 78% with no cytotoxicity, while I. verum was not effective. Furthermore, we characterized the EOs and their vapor phase by the head-space gas chromatography–mass spectrometry technique, observing that the major component found in each liquid EO is the same one of the corresponding vapor phases, with the exception of M. alternifolia. To deepen the mechanism of action, the morphological integrity of virus particles was checked by negative staining transmission electron microscopy, showing that they interfere with the lipid bilayer of the viral envelope, leading to the decomposition of membranes. We speculated that the most abundant components of the vapor EOs might directly interfere with influenza virus envelope structures or mask viral structures important for early steps of viral infection.

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Screening of the essential oil effects on human H1N1 influenza virus infection: an in vitro study in MDCK cells

Cited by 7 publications

References 14 publications

The Potential Use of Plant Growth Regulators for Modification of the Industrially Valuable Volatile Compounds Synthesis in Hylocreus undatus Stems

The Potential Use of Plant Growth Regulators for Modification of the Industrially Valuable Volatile Compounds Synthesis in Hylocreus undatus Stems

Antibacterial Activity of Ethyl Acetate Extract of Endophytic Fungus (Paraconiothyrium Brasiliense) Through Targeting Pathogenesis Related Dihydropteroate Synthase (DHPS)

Ultrastructural Damages to H1N1 Influenza Virus Caused by Vapor Essential Oils

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