Screening of the antifungal potential of nudibranch Chromodoris lineolata associated bacteria against Candida albicans

Wijaya, A P; Sabdono, Agus; Sibero, Mada Triandala; Trianto, Agus; Radjasa, Ocky Karna

doi:10.1088/1755-1315/967/1/012043

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…PCR amplification of partial l6S rRNA gene of selected Gorgonian-associated bacteria, purification of PCR products and subsequent sequencing analysis were performed according to Wijaya et al (2022) methods.…”

Section: S Rrna Gen-pcr Amplificationmentioning

confidence: 99%

Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

Sabdono¹,

Lestari²,

Sibero³

2022

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Gorgonian corals of Karimunjawa are impacted by anthropogenic activities, such as increasingly high mariculture intensity with consequent eutrophication, overfishing, tourism, sewage, and other pollutant discharges, which result in changes in the microbial community structure. In this study, bacterial communities associated with six species of Gorgonian, Viminella sp., Ellisella sp., Antipathes sp., Melithaea sp., Astrogorgia sp., and Junceella sp. from both the Marine Protected Area (MPA) and non-Marine Protected Area (non-MPA) zones were screened for their antipathogenic potential against Urinary Tract Infections (UTIs) pathogens. The selected bacterial isolates were identified and compared for their abundance and diversity between the two zones. A total of 156 bacterial strains were assayed for their prospective antipathogenic compounds against seven UTI pathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus saptophyticus, Acinetobacter baumannii, Klebsiella pneumonia, and Candida albicans. The results showed that 17 of 92 (18.48%) and 6 of 64 (9.37%) bacterial isolates from MPA and non-MPA, respectively, exhibited antimicrobial activity in at least one of the UTI pathogens. By analyzing the gene of 16S rRNA, it was discovered that the 17 isolates of MPA were associated with phyla Actinobacteria, Firmicutes and Proteobacteria, including Streptomyces zhaozhoue, Nocardiopsis salina, Micrococcus endophyticus, Brevibacterium casei, Micrococcus yunnanensis, Saccharopolyspora coralli, Bacillus paramycoides, Virgibacillus salarius, Oceanobacillus iheyensis, and Vibrio alginolyticus. In contrast, only six selected isolates of non-MPA were associated with the phyla Actinobacteria and Proteobacteria, including Nocardiopsis salina, Micrococcus yunnanensis, and Acinetobacter soli. The Diversity Index (H’), Species Richness (S), and Relative Abundance of the MPA zone were higher than those of non-MPA. These results demonstrated that Gorgonian octocoral species in the MPA region harbour varied bacteria and we propose that many Gorgonian-associated bacteria have the prospective for advancing broad-spectrum antibiotics.

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Section: S Rrna Gen-pcr Amplificationmentioning

confidence: 99%

Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

Sabdono¹,

Lestari²,

Sibero³

2022

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"BACTERIAL DIVERSITY OF THE GORGONIAN CORAL Plexaura sp.: SCREENING FOR ANTI-PATHOGENIC PROPERTY AGAINST NOSOCOMIAL PATHOGENIC Acinetobacter baumannii "

LARASATI¹,

Trianto²,

Radjasa³

et al. 2023

Int J Conserv Sci

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"Chronic infectious diseases caused by biofilm-forming pathogenic bacteria are the second rank causes of death in Indonesia after heart and blood vessel diseases. The emergence of antibiotic-resistant Acinetobacter baumannii pathogenic bacteria worldwide poses the main medicinal challenge to the search for new antibiotics. However, the use of marine invertebrates as raw material sources is not ecologically friendly due to the low concentration of bioactive compounds in marine invertebrates. This study aimed to investigate gorgonian Plexaura sp.-associated bacteria that have the potential as anti nosocomial pathogenic A. baumannii. A total of 29 gorgonian Plexaura sp.-associated bacterial isolates were screened for their antibacterial activity against nosocomial pathogenic A. baumannii. The 5 bacterial isolates exhibited antipathogenic activity against A. baumannii. The RA17-2 isolate showed the highest diameter size of the inhibition zone. The 16S rRNA sequence analyses revealed that these 5 isolates were closely related to Bacillus, Virgibacillus, and Nitratireductor. None of the 5 antipathogenic isolates possess PKS-I, PKS-II, and NRPS genes, except the PKS-I gene of the RA17-2 isolate. These results showed that Plexauridae sp is a potential source for the development of antibiotic drugs."

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Biogeographic assessment of Gorgonian associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

Sabdono¹,

Lestari²,

Sibero³

2022

Preprint

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Gorgonian corals of Karimunjawa are impacted by anthropogenic activities, such as increasing high mariculture intensity with consequent eutrophication, overfishing, tourism, sewage, and other pollutant discharge, which result in changes in the microbial community structure. In this study, bacterial communities associated with six species of gorgonian, Viminella sp., Ellisella sp., Antipathes sp., Melithaea sp., Astrogorgia sp, and Junceella sp. from both the Marine Protected Area (MPA) and non-Marine Protected Area (non-MPA) zones were screened for their antipathogenic potential against Urinary Tract Infections (UTIs) pathogens. The selected bacterial isolates were identified and compared for their abundance and diversity between the two zones. 156 bacterial strains were assayed for their prospective antipathogenic compounds against seven UTI pathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus saptophyticus, Acinetobacter baumannii, Klebsiella pneumonia, Candida albicans. The result showed that 17 of 92 (18.48%) and 6 of 64 (9.37 %) bacterial isolates from MPA and non-MPA, respectively, exhibited antimicrobial activity in at least one UTIs pathogens. Analyzing the gene of 16S rRNA discovered that the 17 isolates of MPA were associated with phyla Actinobacteria, Firmicutes, and Proteobacteria, including Streptomyces zhaozhoue, Nocardiopsis salina, Micrococcus endophyticus, Brevibacterium casei, Micrococcus yunnanensis, Saccharopolyspora coralli, Bacillus paramycoides, Virgibacillus salarius, Oceanobacillus iheyensis, Vibrio alginolyticus. In contrast, only six selected isolates of non-MPA were associated with phyla Actinobacteria and Proteobacteria, including Nocardiopsis salina, Micrococcus yunnanensis, and Acinetobacter soli. The diversity index (H'), species richness (S), and relative abundance of the MPA zone were higher than that of non-MPA. These results demonstrated that gorgonian octocoral species in the MPA region harbor varied bacteria and propose that many gorgonian-associated bacteria have the prospective for advancing broad-spectrum antibiotics.

show abstract

Screening of the antifungal potential of nudibranch Chromodoris lineolata associated bacteria against Candida albicans

Cited by 3 publications

References 43 publications

Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

"BACTERIAL DIVERSITY OF THE GORGONIAN CORAL Plexaura sp.: SCREENING FOR ANTI-PATHOGENIC PROPERTY AGAINST NOSOCOMIAL PATHOGENIC Acinetobacter baumannii "

Biogeographic assessment of Gorgonian associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

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Screening of the antifungal potential of nudibranch Chromodoris lineolata associated bacteria against Candida albicans

Cited by 3 publications

References 43 publications

﻿Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

﻿Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

"BACTERIAL DIVERSITY OF THE GORGONIAN CORAL Plexaura sp.: SCREENING FOR ANTI-PATHOGENIC PROPERTY AGAINST NOSOCOMIAL PATHOGENIC Acinetobacter baumannii "

Biogeographic assessment of Gorgonian associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

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Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia

Biogeographic assessment of Gorgonian-associated bacteria with antipathogenic Urinary Tract Infections (UTIs) in Karimunjawa Marine National Park, Java Sea, Indonesia