Screening of target-specific olfactory receptor and development of olfactory biosensor for the assessment of fungal contamination in grain

Ahn, Jung Ho; Lim, Jong Hyun; Park, Juhun; Oh, Eun Hae; Son, Min Ho; Hong, Seunghun; Park, Tai Hyun

doi:10.1016/j.snb.2014.12.060

Cited by 34 publications

(21 citation statements)

References 45 publications

(5 reference statements)

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“…Recently, several types of the bioelectronic nose have been developed for applications in various fields [1, 22, 30, 41, 54] including biomedical and industrial purposes such as disease diagnosis, food quality assessment, and environmental monitoring. In contrast to vision and hearing, smell cannot be exactly described or quantified because the classification and description of smell is quite subjective and abstractive.…”

Section: Introductionmentioning

confidence: 99%

Bioelectronic nose and its application to smell visualization

Ko¹,

Park

2016

J Biol Eng

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There have been many trials to visualize smell using various techniques in order to objectively express the smell because information obtained from the sense of smell in human is very subjective. So far, well-trained experts such as a perfumer, complex and large-scale equipment such as GC-MS, and an electronic nose have played major roles in objectively detecting and recognizing odors. Recently, an optoelectronic nose was developed to achieve this purpose, but some limitations regarding the sensitivity and the number of smells that can be visualized still persist. Since the elucidation of the olfactory mechanism, numerous researches have been accomplished for the development of a sensing device by mimicking human olfactory system. Engineered olfactory cells were constructed to mimic the human olfactory system, and the use of engineered olfactory cells for smell visualization has been attempted with the use of various methods such as calcium imaging, CRE reporter assay, BRET, and membrane potential assay; however, it is not easy to consistently control the condition of cells and it is impossible to detect low odorant concentration. Recently, the bioelectronic nose was developed, and much improved along with the improvement of nano-biotechnology. The bioelectronic nose consists of the following two parts: primary transducer and secondary transducer. Biological materials as a primary transducer improved the selectivity of the sensor, and nanomaterials as a secondary transducer increased the sensitivity. Especially, the bioelectronic noses using various nanomaterials combined with human olfactory receptors or nanovesicles derived from engineered olfactory cells have a potential which can detect almost all of the smells recognized by human because an engineered olfactory cell might be able to express any human olfactory receptor as well as can mimic human olfactory system. Therefore, bioelectronic nose will be a potent tool for smell visualization, but only if two technologies are completed. First, a multi-channel array-sensing system has to be applied for the integration of all of the olfactory receptors into a single chip for mimicking the performance of human nose. Second, the processing technique of the multi-channel system signals should be simultaneously established with the conversion of the signals to visual images. With the use of this latest sensing technology, the realization of a proper smell-visualization technology is expected in the near future.

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Section: Introductionmentioning

confidence: 99%

Bioelectronic nose and its application to smell visualization

Ko¹,

Park

2016

J Biol Eng

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“…Since then, this strategy has been used for detecting different odorants as well as sweet and umami tastants [200–202]. Vesicle-based biosensors have also demonstrated utility for other applications beyond taste and smell, including the detection of lung cancer biomarkers [203], neurotransmitters [204], and fungal contaminants [205]. …”

Section: Cell Membrane-based Nanostructuresmentioning

confidence: 99%

Cell membrane-derived nanomaterials for biomedical applications

et al. 2017

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The continued evolution of biomedical nanotechnology has enabled clinicians to better detect, prevent, manage, and treat human disease. In order to further push the limits of nanoparticle performance and functionality, there has recently been a paradigm shift towards biomimetic design strategies. By taking inspiration from nature, the goal is to create next-generation nanoparticle platforms that can more effectively navigate and interact with the incredibly complex biological systems that exist within the body. Of great interest are cellular membranes, which play essential roles in biointerfacing, self-identification, signal transduction, and compartmentalization. In this review, we explore the major ways in which researchers have directly leveraged cell membrane-derived biomaterials for the fabrication of novel nanotherapeutics and nanodiagnostics. Such emerging technologies have the potential to significantly advance the field of nanomedicine, helping to improve upon traditional modalities while also enabling novel applications.

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“…In artificial cell-based platforms, ORs isolated from cells are inserted into artificially constructed lipid membranes such as liposomes and nanodiscs (Figure 4B,C). Briefly, Ahn et al expressed olfactory receptors in a host cell and produced nanovesicles by simple agitation [66]. These nanovesicles were immobilized on a carbon nanotube and the binding of odorant was electrically detected.…”

Section: Potential Applications Of Transmembrane Proteinsmentioning

confidence: 99%

Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications

Ryu

Fuwad

Yoon

et al. 2019

IJMS

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In biological cells, membrane proteins are the most crucial component for the maintenance of cell physiology and processes, including ion transportation, cell signaling, cell adhesion, and recognition of signal molecules. Therefore, researchers have proposed a number of membrane platforms to mimic the biological cell environment for transmembrane protein incorporation. The performance and selectivity of these transmembrane proteins based biomimetic platforms are far superior to those of traditional material platforms, but their lack of stability and scalability rule out their commercial presence. This review highlights the development of transmembrane protein-based biomimetic platforms for four major applications, which are biosensors, molecular interaction studies, energy harvesting, and water purification. We summarize the fundamental principles and recent progress in transmembrane protein biomimetic platforms for each application, discuss their limitations, and present future outlooks for industrial implementation.

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Screening of target-specific olfactory receptor and development of olfactory biosensor for the assessment of fungal contamination in grain

Cited by 34 publications

References 45 publications

Bioelectronic nose and its application to smell visualization

Bioelectronic nose and its application to smell visualization

Cell membrane-derived nanomaterials for biomedical applications

Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications

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