Screening of T7 phage displayed Bactrocera dorsalis (Hendel) antenna cDNA library against chemosensory protein

Abstract

Recent studies have shown that chemosensory proteins (CSPs) were involved in diverse life activities such as insect feeding, development, mating, immune regulation, as well as other important circadian rhythms, etc. To screen the proteins involved in the BdorCSP-related physiological activity, a cDNA library of the Bactrocera dorsalis (Hendel) antenna expressed on the surface of T7 phage was screened against BdorCSP. After four rounds of screening, ELISA-positive samples of selected phages were sequenced and identified as protein disulfide isomerase (PDI), trypsin-like serine protease (Ser), TakeOut (TO), and a new protein by GenBank blast, respectively. Real-time quantitative PCR results showed that the expression levels of Ser, TO, and the new protein were the highest in antenna, sharing similar expression pattern with BdorCSP. These results reveal that these proteins might be involved in the BdorCSP-related physiological or metabolic activities. This work paves a new way for exploring the function of CSPs.

Molecular Cloning, Expression and Molecular Modeling of Chemosensory Protein from Spodoptera litura and Its Binding Properties with Rhodojaponin III

“…Later studies identified other members of this family in sensory appendages such as antennae, labial palps and legs in a variety of insects [10] – [11] . Several members of this class of protein have been described in insects of different orders, including Lepidoptera [11] – [19] , Orthoptera [10] , [20] – [22] , Hymenoptera [7] , [23] – [26] , Diptera [27] , Blattoidea [28] – [29] , Phasmatodea [30] – [32] , Hemiptera [33] , etc. The function of CSPs as carrier proteins was strengthened by studies on the higher order structure of a CSP from Bombyx mori , which revealed a globular configuration of six alpha helices surrounding a hydrophobic binding pocket [34] .…”