Screening of synthetic phosphodiesterase‐5 inhibitors in herbal dietary supplements using transmission‐mode desorption electrospray and high‐resolution mass spectrometry

Screening of Phosphodiesterase-5 Inhibitors and Their Analogs in Dietary Supplements by Liquid Chromatography–Hybrid Ion Trap–Time of Flight Mass Spectrometry

“…The retention times and mass errors of all analytes are listed in Table 1, and the extracted ion chromatograms are depicted in Figure 1. (6), piperidinohongdenafil (7), homosildenafil (8), dimethylsildenafil (9), udenafil (10), cyclopentinafil (11), thiosildenafil (12), hydroxythiohomosildenafil (13), thiohomosildenafil (14), dimethylthiosildenafil (15), oxohongdenafil (16), benzylsildenafil (17), hydroxychlorodenafil (18), chlorodenafil (19), nitrodenafil (20), nor-neosildenafil (21), dichlorodenafil (22), vardenafil (23), acetylvardenafil (24), hydroxyvardenafil (25), nor-neovardenafil (26), desulfovardenafil (27), pseudovardenafil (28), tadalafil (29), aminotadalafil (30), acetaminotadalafil (31), demethyltadalafil (32), chloropretadalafil (33), N-octyltadalafil (34), yohimbine (35), mirodenafil (36), thioquinapiperifil (37), xanthoanthrafil (38), and phenolphthalein (39). * indicated the analytes among multiple peaks in the EIC.…”

“…In this regard, various spectrometric and chromatographic methods have been adopted to analyze the illegally added drugs in complex matrices [ 3 , 4 , 5 , 9 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. High-performance liquid chromatography with diode array detection (HPLC–DAD) and liquid chromatography with mass spectrometry (LC–MS) have been previously used to detect PDE-5 inhibitors and their analogs [ 3 , 5 , 7 , 8 , 9 , 10 , 16 , 17 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. When the standards of PDE-5 inhibitors and their analogs are available, HPLC with a UV spectrometer is a simple method for quantitative analysis [ 11 ].…”