Screening of selected improved cassava varieties for bacterial blight and varietal performance under natural environment in Ibadan, south-western Nigeria

Azorji, J. N.; Igwe, C. E.; Kanu, C.

doi:10.18801/jmsmr.010216.08

Cited by 1 publication

(1 citation statement)

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“…The tissues were macerated using sterile glass rods in 5 mL sterile distilled in universal bottles. The macerate was then streaked on yeast peptone glucose agar (YPGA) [13] and incubated for 24 h. The bacterial colonies were purified and subjected to morphological, biochemical, and pathogenicity tests to identify and confirm the CBB causal agents [8,[14][15][16]. The biochemical characteristics determined included Gram stain, potassium hydroxide solubility, utilization of sucrose, lactose cellobiose, and catalase test.…”

Section: Isolation and Confirmation Of Cbb Causal Agentsmentioning

confidence: 99%

Effect of farmers’ knowledge and production practices on the intensity of cassava bacterial blight in Western Kenya

Okoth,

Muthomi,

Mwang’ombe

2024

J App Biol Biotech

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The study was conducted to determine the association between farmers’ knowledge and production practices on the intensity of cassava bacterial blight (CBB) in Western Kenya. Multistage sampling was used to select 193 farms in Nambale and Teso south sub-counties in Western Kenya. A semi-structured questionnaire was administered to obtain information on farmer’s knowledge and cassava production practices. Within each farm, bacterial blight incidence was determined and 30 cassava plants were evaluated for disease severity. Symptomatic leaf samples were collected for isolation and confirmation of the CBB pathogens. GPS coordinates of each farm were taken for the development of disease distribution maps. Data analysis included descriptive statistics and the Chi-square test which was used to determine the association between sociodemographic traits and disease incidence. CBB was prevalent in both sub-counties and Xanthomonas phaseoli pv manihotis was the more widespread compared to Xanthomonas axonopodis pv cassavae. However, there was no association between CBB incidence, training, seed source, and intercropping suggesting that other factors contributed to the high prevalence of the disease. About 85% of the farmers interviewed were unaware of the disease suggesting that the farmers could be spreading the disease unknowingly through the use of self-recycled and neighbor-obtained cassava cuttings. The findings of this study will contribute toward measures aimed at curbing the disease and its spread.

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