Screening of salinity tolerance of rice at early seedling stage

Mondal, Satyen; Borromeo, Teresita H.

doi:10.18801/jbar.100116.102

Cited by 21 publications

(11 citation statements)

References 4 publications

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“…However, the IC50 EC is still higher than that at which the yield is drastically reduced (Akbar and Senadhira, 1985). The importance of going for short term (72 h) IC50 determination is that it gives an indication of the breeding lines that should be considered for salt tolerance testing by the long term exposure testing methods already available (Rekha et al, 2018;Bado et al, 2016), instead of testing all the genotypes, which may be more than 200 in numbers (Mondal and Borromeo, 2016), by long term exposure. For example, the cultivar Naveen can outright be opted out for the long term exposure testing, as its 72 h IC50 is 11.38 dS/m, almost equivalent to the value (10 dS/m) at which the plant dies before maturity (Munns et al, 2006).…”

Section: B Comparative Analysis Of Ic50 For Seedling Shoot Growth Inmentioning

confidence: 99%

“…Even Swarna can be opted out of the long term exposure testing because of showing arrest in growth from 256 mM onwards, a concentration little higher than the IC50 value for shoot length inhibition. Screening of 231 rice genotypes for salt tolerance at the seedling stage by long term exposure method has shown that only 4 and 18 genotypes were tolerant and moderately tolerant, respectively, and the rest were sensitive to highly sensitive (Mondal and Borromeo, 2016). Initial performance of 72 h IC50 test for shoot inhibition could have thus allowed omission of at least 90 % of the genotype for long term exposure testing and visual scoring.…”

Section: B Comparative Analysis Of Ic50 For Seedling Shoot Growth Inmentioning

confidence: 99%

“…Screening of germplasm for salt tolerance at the seedling stage is in practice. The criteria used for screening are visual salt-induced effects like growth (height), leaf injury, and seedling survival over two weeks (Gregorio et al, 1997;Islam and Karim, 2010;Mondal and Borromeo, 2016). However, the screening tests do not provide any quantifiable unit of salt toxicity or tolerance.…”

Section: Introductionmentioning

confidence: 99%

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NaCl Toxicity Testing in Rice Cultivars at the Seedling Stage: Evaluating the Applicability of IC50 as a Method using Hydroponic Medium

Gupta¹,

Shaw²

2021

JSR

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Salt tolerant cultivars of rice are continuously being developed for suitability of their cultivation in salt affected lands that cause significant loss in the crop yield. Testing of salt tolerance of a large number of breeding lines generated after crossing and selfing is a major constrain in the development of salt tolerant cultivars. The present study describes a laboratory based method of testing salt tolerance of the breeding lines at the seedling stage. Shoot growth inhibition data were generated for five rice cultivars after hydroponic exposure of the 6 days old seedlings to 0.5%, 1.0%, 1.5 %, and 2.0% for 24, 48, and 72 h. Tukey-Kramer's test revealed a significant difference in shoot growth inhibition between treatment concentrations for each of the cultivars. The IC50 values for NaCl varied from 153 mM (0.5%) for the salt-sensitive Naveen to 284 mM (1.5%) for the salt-tolerant Pokkali. IC50 values demonstrated a difference in salt-tolerance/sensitivity of the rice cultivars, and hence the method can be used for testing salt tolerance of the breeding lines in the shortest possible time. Moreover, the method provides a quantitative value, rather than mere phenotypic observations being followed in the prevailing method of screening of salt tolerance of breeding lines.

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Section: B Comparative Analysis Of Ic50 For Seedling Shoot Growth Inmentioning

confidence: 99%

Section: B Comparative Analysis Of Ic50 For Seedling Shoot Growth Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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NaCl Toxicity Testing in Rice Cultivars at the Seedling Stage: Evaluating the Applicability of IC50 as a Method using Hydroponic Medium

Gupta¹,

Shaw²

2021

JSR

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“…During the early seedling (2-3 leaf) stage, rice remains very sensitive studied by (Mondal and Borromeo, 2016). Salinity reduces seedling survival and results in low crop standing (Pushpam and Rangasamy, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of Salinity on Emergence and Early Growth Stages of Aromatic and Non-Aromatic Rice (Oryza sativa L.) Genotypes

Channa

Mahar

Rajpar

et al. 2021

Pakistan Journal of Scientific & Industrial Research

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Salinity hampers the production of many field crops in the region including rice (Oryza sativa L.), while commonly classified as salt sensitive within the same species, the level of sensitivity varies between genotypes. This study investigated the salinity tolerance of 28 rice genotypes, including 9 aromatics and 19 non-aromatics. Sixty seeds of each genotype were initially sown in trays (24x18 inches) during the 1st week of June, by of using four salt treatments (0, 40, 80 and 120 mM NaCl+CaCl2 @ 20:1). The experiment was laid down in a completely randomized design with four replicates in laboratory conditions, at Shah Abdul Latif University Khairpur, Sindh, for the period of twenty-five days. A significant reduction in agro-morphological parameters was observed against all salinity levels. Based on reduction in dry matter yield, all rice genotypes were found tolerant at 40 mM. Eleven rice genotypes were found tolerant, fourteen were moderately tolerant, one was moderately sensitive and remaining two genotypes were found sensitive at 80 mM salinity level. Furthermore, none of the genotypes were able to with stand 120 mM of salinity. The genotypes Khushboo, DR-83 and Mahek performed meager and showed more than 50% reduction over control and categorised as sensitive, with the genotypes Latifee, DR-67 and DR-92, DR-51 and IR-6 are categorised as tolerant with a reduction of less than 20% over control based on dry matter yield reduction against all salinity treatments at the early seedling stage. However, these genotypes cannot be justified as tolerant only on the basis of their improved performance at early growth stage. Hence, these genotypes are suggested to be studied further at other advanced growth stages up to maturity to evaluate their response under a saline environment.

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“…Applying molecular markers in selection is a modern breeding method increasing the speed of the selection process, thus increasing the efficiency of gene selection per unit of time (Hospital, 2003). Some previous studies to select rice for salinity tolerance by molecular markers were done (Mondal and Borromeo, 2016;Huyen et al 2013;Thomson et al 2010). Hereditary analysis to evaluate the relationship between or among individuals in a population and parents is also important as well.…”

mentioning

confidence: 99%

Genetic Analysis of Salinity Tolerance in a Rice Backcross Population at Seedling Stage

Tam¹,

Tu²,

Ut³

et al. 2019

J biosci agric res

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Rice breeding of salinity tolerance based on a combination of phenotyping, genotyping and analyzing of genetic relationship was aiming to create the next generation with stable tolerance. In this study, 99 rice lines of the OMCS2000*4/Pokkali population in BC3F2 generation were screened in saline environments of 0, 6 and 12 dS m-1 at seedling stage. The tolerant lines were continuously tested the Saltol gene with 2 tightly markers, RM3412 and RM8094. The population was employed for the assessment of correlation, genetic variability, heritability and genetic advance for the related traits. Through phenotyping and genotyping, the population was tolerant to 6 dS m-1 for 4 weeks and 12 dS m-1 for 2 weeks, in which, 21/99 rice lines was highly tolerant and carried the Saltol gene. In this population, SES score and fresh biomass were the most significant traits and could explain over 70% of variability. SES scores were negatively correlated to almost all other traits, strongly to biomass, moderately to plant height, and lowly to root length. Analysis of variance revealed that sufficient genetic variation has been created for all traits. Relative magnitude of phenotypic coefficients of variation was higher than genotypic coefficients of variation for all the characters under study indicating environmental influence on the traits. High heritability coupled with high genetic advance was almost traits, exception of dry biomass. These indicated that direct selection can be effective for salinity tolerance improvement in the population under study.

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Screening of salinity tolerance of rice at early seedling stage

Cited by 21 publications

References 4 publications

NaCl Toxicity Testing in Rice Cultivars at the Seedling Stage: Evaluating the Applicability of IC50 as a Method using Hydroponic Medium

NaCl Toxicity Testing in Rice Cultivars at the Seedling Stage: Evaluating the Applicability of IC50 as a Method using Hydroponic Medium

Effect of Salinity on Emergence and Early Growth Stages of Aromatic and Non-Aromatic Rice (Oryza sativa L.) Genotypes

Genetic Analysis of Salinity Tolerance in a Rice Backcross Population at Seedling Stage

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