Screening of salbutamol residues in swine meat and animal feed by an enzyme immunoassay in Taiwan

Sheu, Shi-Yuan; Yu, Lei; Tai, Yung-Te; Chang, Tang‐Hsien; Kuo, Ting‐Shen

doi:10.1016/j.aca.2009.09.026

Cited by 56 publications

(25 citation statements)

References 11 publications

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“…Conventional analyses of clenbuterol including chromatography method, ELISA, electrophoresis, and electrochemistry involve complex time-consuming steps (such as incubation and repeated washings) can take up to 72 h for a single sample, with sensitivity detection at ppb levels [27][28][29][30][31][32]; thus, there is an urgent need for a highly sensitive sensor to detect clenbuterol to ensure food safety. However, the detection of small molecule clenbuterol (Mw = 277) by a mass transducer using the direct immunoassay is challenging.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…Therefore, SAL has also been illegally used in animal husbandry to enhance the lean-to-fat ratio in meat-producing animals with the resulting substantially increased economic profit (Garssen et al 1995). Studies have shown that accumulation of β 2 -agonist residues in edible tissues of treated animals results in detrimental effects on the central nervous system and cardiovascular of consumers, especially in patients with preexisting heart complications and those in the third trimester (Wang and Shen 2007;Sheu et al 2009). Thus SAL has been inhibited as an additive used in animal feeds for livestock production in many countries such as in China and the European Union.…”

Section: Introductionmentioning

confidence: 98%

“…hinder them to be widely applied in the detection of SAL on-site and rapidly. Meanwhile, immunoassays based on the specific reaction between an antibody (Ab) and its antigen such as flow injection chemiluminescence (FICL) (Xu et al 2015), enzyme-linked immunosorbent assay (ELISA) (Sheu et al 2009), electrochemiluminescence (ECL) (Zhang et al 2014), and radio immunoassay (RIA) (Collins et al 1994) have been used for detection of SAL in different matrices. Above mentioned immunoassays have been broadly applied in lab studies and clinical examination, but the disadvantages such as laborious incubation and rinsing procedures cannot be ignored.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive and Specific Detection of Salbutamol in Swine Feed, Meat, and Urine Samples by a Competitive Immunochromatographic Test Integrated with Surface-Enhanced Raman Scattering

et al. 2016

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Salbutamol (SAL), a kind of β-agonist which can enhance the lean meat-to-fat ratio, has been inhibited as an additive used in animal feeds for livestock production in many countries due to its harmful effect to the consumers. In this study, an ultrasensitive and specific competitive immunochromatographic test (ICT) integrated with surfaceenhanced Raman scattering (SERS) for the detection of SAL was described. The immunoprobe was prepared by immobilizing polyclonal antibody against SAL on the surface of Au@Ag nanoparticles in which the Raman reporter (4-mercaptobenzoic acid, MBA) had been sandwiched. After ICT procedures, the specific SERS signals generated from MBA on the test line of the ICT strip were measured for the quantitative determination of SAL. The assay was completed in 15 min. The IC 50 and the limit of detection (LOD) values of the assay for SAL were 0.028 ng mL −1 and 3.0 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with other three β-agonists (clenbuterol, phenylethanolamine A, and ractopamine), showing high specificity of the assay. Spiking experiments indicated that the average recoveries (n = 3) of SAL from swine feed, meat, and urine samples were in ranges of 98.4-105.2 % with the relative standard deviations (RSDs) of 1.7-7.8 %. The results demonstrated that the proposed ICT was a feasible method for ultrasensitive and specific detection SAL in swine feed, meat and urine samples, and could be extended for the detection of other target analytes.

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“…In order to analysis of SAL in biological samples, several methods have been developed, such as high performance liquid chromatography (HPLC) [1,7,8], capillary electrophoresis (CE) [9][10][11], gas chromatography-mass spectrometry (GC-MS) [12], liquid chromatography-mass spectrometry (LC-MS) [13,14], enzyme immunoassay [15], and bromatometric methods [16]. However, they are suffered to achieve separation of target compounds from complex sample matrices directly without the pretreatment process.…”

Section: Introductionmentioning

confidence: 99%

Screening, recognition and quantitation of salbutamol residues in ham sausages by molecularly imprinted solid phase extraction coupled with high-performance liquid chromatography–ultraviolet detection

Yan

Wang

Han

et al. 2012

Journal of Chromatography B

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Screening of salbutamol residues in swine meat and animal feed by an enzyme immunoassay in Taiwan

Cited by 56 publications

References 11 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Ultrasensitive and Specific Detection of Salbutamol in Swine Feed, Meat, and Urine Samples by a Competitive Immunochromatographic Test Integrated with Surface-Enhanced Raman Scattering

Screening, recognition and quantitation of salbutamol residues in ham sausages by molecularly imprinted solid phase extraction coupled with high-performance liquid chromatography–ultraviolet detection

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