Screening of rice (Oryza sativa) cultivars for resistance to rice black streaked dwarf virus using quantitative PCR and visual disease assessment

Abstract

Rice black streaked dwarf virus (RBSDV) causing rice black streaked dwarf disease is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus) in a persistent propagative manner. The disease is considered among the most destructive in rice production in east and southeast Asia. For sustainable control of the disease, planting resistant cultivars is the most economical and efficient method. The virus content in different rice cultivars was quantified using a TaqMan RT‐qPCR assay under greenhouse conditions and the disease was visually assessed in these cultivars in both greenhouse and field conditions. Results revealed significant positive moderate correlation (r = 0.3787; P = 0.0009) between the virus content and visual disease assessment in the greenhouse under forced inoculation. Among 66 rice cultivars, there was no significant difference in RBSDV genome equivalent copies (GEC) in seven cultivars, namely Lian‐dao 9805 (200.2 ± 12), Liangyou 3399 (206.6 ± 28), Ningjing 4 (206.6 ± 28), DaLiang 207 (302.0 ± 61), X 008 (354.0 ± 30), Shengdao 301 (658.4 ± 69) and Liangyou 1129 (679.5 ± 98). These cultivars were also visually assessed as resistant under greenhouse and field conditions. These cultivars could be used in disease management to reduce the likelihood of epidemics.

Analysis of Sogatella furcifera proteome that interact with P10 protein of Southern rice black-streaked dwarf virus

“…Nonviruliferous WBPH ( S. furcifera ), originally captured in Nanjing, China, were reared in glass beakers with rice seedlings in a laboratory incubator at 26 °C with 16 h light/8 h dark. The insects were transferred onto fresh rice seedlings every week 60 .…”

Development of a reverse-transcription droplet digital PCR method for quantitative detection of Cucumber green mottle mosaic virus

“…Due to the lack of the standard virus samples for the majority of plant viruses, the precise quantification methods are not available for the majority of plant viruses in most common laboratories, dampening the quantitative detection of plant viruses and thus increasing the risk of their spread and outbreaks of in the fields. Breeding and cultivation of the virus-resistant varieties are critical for controlling plant viral disease ( Hajano et al, 2016 ); however, selection of virus-resistant varieties during breeding programs also requires the straightforward quantitative methods for detecting the viruses and evaluating the virus resistance of breeding materials ( Picó et al, 2005 ).…”

Flavonone 3-hydroxylase Relieves Bacterial Leaf Blight Stress in Rice via Overaccumulation of Antioxidant Flavonoids and Induction of Defense Genes and Hormones

“…We focused on BLB because it is a devastating disease and greatly reduces the yield and quality of rice. Reports have shown that a mild BLB infection can reduce the yield by up to 20%, whereas severe infection reduces the yield by up to 50% [ 41 ]. The rate of infection of BLB depends on the strain, rice cultivar, geographical location, stage of plant growth, and seasonal variations [ 42 ].…”