Screening of rice germplasm and validation of markers for high temperature stress tolerance using morphologic traits and molecular markers

Mohapatra, SD; Barik, SR; Meher, Jitendriya; Patra, B. C.; Pradhan, Sanatan

doi:10.5958/2249-5266.2018.00014.0

Cited by 3 publications

(2 citation statements)

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“…(2013) ; Pradhan et al. (2016) , and Mohapatra et al., 2021 . Sunoharaa et al., (2016) reported that RM3475 and RM237 were associated with controlling panicle characters.…”

Section: Discussionmentioning

confidence: 99%

“…Xiao et al (2011) associated RM471 with QTLs influencing seed set percentage. The marker RM242 was reported to influence the erect panicle trait (Kong et al, 2007) and was also associated with plant height, panicle length, and spikelet fertility under heat stress by Wei et al (2013); Pradhan et al (2016), and Mohapatra et al, 2021. Sunoharaa et al, (2016 reported that RM3475 and RM237 were associated with controlling panicle characters.…”

Section: Polymorphic Markers Related To High Temperature Tolerancementioning

confidence: 99%

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Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma

et al. 2023

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The damage caused by high temperature is one of the most important abiotic stress affecting rice production. Reproductive stage of rice is highly susceptible to high temperature. The present investigation was undertaken to identify polymorphic microsatellite markers (SSR) associated with heat tolerance. The rice cultivars NERICA– L 44 (heat tolerant) and Uma (heat susceptible) were crossed to generate F1 and F2 populations. The F2 population was subjected to heat stress at >38°C and the 144 F2 plants were evaluated for their tolerance. The results note that the mean of the F2 population was influenced by the tolerant parent with regards to the traits of plant height, membrane stability index, photosynthetic rate, stomatal conductance, evapotranspiration rate, pollen viability, spikelet fertility and 1000 grain weight. Ten each of the extremely susceptible and tolerant plants were selected based on the spikelet fertility percentage. Their DNA was pooled into tolerant and susceptible bulks and Bulked Segregant Analysis (BSA) was carried out using 100 SSR markers to check for polymorphism. The survey revealed a polymorphism of 18% between the parents. RM337, RM10793, RM242, RM5749, RM6100, RM490, RM470, RM473, RM222 and RM556 are some of the prominent markers that were found to be polymorphic between the parents and the bulks. We performed gene annotation and enrichment analysis of identified polymorphic markers. Result revealed that the sequence specific site of that chromosome mostly enriched with biological processes like metabolic pathway, molecular mechanism, and subcellular function. Among that RM337 was newly reported marker for heat tolerance. Expression analysis of two genes corresponds to RM337 revealed that LOP1 (LOC_Os08g01330) was linked to high temperature tolerance in rice. The results demonstrate that BSA using SSR markers is useful in identifying genomic regions that contribute to thermotolerance.

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“…(2013) ; Pradhan et al. (2016) , and Mohapatra et al., 2021 . Sunoharaa et al., (2016) reported that RM3475 and RM237 were associated with controlling panicle characters.…”

Section: Discussionmentioning

confidence: 99%

Section: Polymorphic Markers Related To High Temperature Tolerancementioning

confidence: 99%

Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma

et al. 2023

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Genetic structure and molecular markers-trait association for physiological traits related to seed vigour in rice

et al. 2021

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Genetic variability and association of microsatellite markers with seed physiological traits related to seed vigour in rice (Oryza sativa L.)

Kumari,

Chakrabarty,

Singh

et al. 2023

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High seed vigour is vital for successful nursery management and crop establishment. Genetic variability in seed vigour traits and association with 41 SSR markers in 44 Indian rice varieties was studied.Analysis of variance indicated presence of high genetic variability, heritability, and genetic advance.Selecting for these traits would be effective for seed vigour improvement.Among 41 markers, 39 were polymorphic.Out of 141 alleles detected 138 were polymorphic.The number of alleles per locus varied from 2 to 5. The overall size of amplified products varied from 50 bp (RM 240) to 600 bp (RM 13). The PIC value of each marker ranged from 0.013 to 0.967, with an average of 0.430. 44 rice varieties were classified into five major clusters based on their genetic similarity values and maturity periods.RM 1353, RM 250 and RM 229 on chromosomes number 7, 2 and 11, respectively were associated with all seed physiological traits.The traits identified and markers detected in the association analysis will be useful for improvement of seed vigour in rice.

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Screening of rice germplasm and validation of markers for high temperature stress tolerance using morphologic traits and molecular markers

Cited by 3 publications

References 0 publications

Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma

Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma

Genetic structure and molecular markers-trait association for physiological traits related to seed vigour in rice

Genetic variability and association of microsatellite markers with seed physiological traits related to seed vigour in rice (Oryza sativa L.)

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