Screening of Propionibacterium spp. for potential probiotic properties

Campaniello, Daniela; Bevilacqua, Antonio; Sinigaglia, Milena; Altieri, Clelia

doi:10.1016/j.anaerobe.2015.06.003

Cited by 25 publications

(23 citation statements)

References 22 publications

(32 reference statements)

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“…9 log cfu per mL for all microorganisms, but only propionibacteria could form a microbial biofilm (4.81 log cfu per cm 2 P. freudenreichii and 6.42 log cfu per cm 2 P. jensenii ) (Fig. ), thus confirming what found on their ability to adhere to IPEC‐J2, a model cell line simulating the intestinal mucosa (Campaniello et al ., ).…”

Section: Resultsmentioning

confidence: 97%

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How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria

Campaniello

Speranza

Petruzzi

et al. 2017

Int J of Food Sci Tech

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Summary Two commercial promising probiotic strains (Propionibacterium jensenii and Propionibacterium freudenreichii) were studied to evaluate two basic probiotic requisites (adhesion and survival during the transit into the gut); two commercial starter cultures (Lactobacillus plantarum and Lactobacillus delbrueckii subsp. bulgaricus) were used as references. As an additional goal, the technological robustness was evaluated as growth as a function of pH, temperature and salt. Adhesion was studied as biofilm formation and hydrophobicity, whereas a simplified protocol was used to simulate the transit into the gut, along with the assessment of survival at pH 2.5 and in the presence of 0.3% bile salts. Propionibacteria were hydrophobic and form biofilm on glass slides, thus confirming the correlation of these properties and with the ability to adhere. None of the strains survived after the simulation of the transit into the gut, but this property could be induced in propionibacteria using a simple protocol of adaptive evolution. This paper suggests the possibility of using hydrophobicity as a screening tool to assess adhesion in propionibacteria and highlights the necessity of using a complete protocol of simulation of the gut to study the resistance to the conditions of stomach and intestine to avoid false‐positive results.

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Section: Resultsmentioning

confidence: 97%

“…Propionibacteria have a wide potential (Altieri, ). Thus, this paper focused on two commercial strains ( P. freudenreichii and P. jensenii ), preliminary shown to possess good probiotic properties (Campaniello et al ., ), to study how assess the two basic probiotic requisites. L. plantarum and L. delbrueckii were used as negative controls.…”

Section: Resultsmentioning

confidence: 99%

How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria

Campaniello

Speranza

Petruzzi

et al. 2017

Int J of Food Sci Tech

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“…Overall, the genus Propionibacterium was the most abundant OTU (22.2%). These are Gram-positive rod-shaped bacteria with probiotic properties in humans, although some species are opportunistic pathogens [51,52]. Another study found that the three dominant bacterial phyla were Tenericutes (44.2%), Proteobacteria (39.22%) and Firmicutes (13.09%), and the genus Spiroplasma was the most abundant OTU (44.1%) [48].…”

Section: Microbial Contaminationmentioning

confidence: 99%

Sustainable farming of the mealworm Tenebrio molitor for the production of food and feed

Grau

Vilcinskas

Joop

2017

Zeitschrift Für Naturforschung C

139

100

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Abstract:The farming of edible insects is an alternative strategy for the production of protein-rich food and feed with a low ecological footprint. The industrial production of insect-derived protein is more cost-effective and energy-efficient than livestock farming or aquaculture. The mealworm Tenebrio molitor is economically among the most important species used for the large-scale conversion of plant biomass into protein. Here, we review the mass rearing of this species and its conversion into food and feed, focusing on challenges such as the contamination of food/feed products with bacteria from the insect gut and the risk of rapidly spreading pathogens and parasites. We propose solutions to prevent the outbreak of infections among farmed insects without reliance on antibiotics. Transgenerational immune priming and probiotic bacteria may provide alternative strategies for sustainable insect farming.

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“…They show high tolerance in vitro to stimulated human upper gastrointestinal tract conditions, depending on species and strain type. The growth or delivery medium may also provide protection [18,19,20,21,22,23,24]. The tolerance response results in various modifications such as morphological changes or proteins expression.…”

Section: Gut Persistence Of Dairy Propionibacteriamentioning

confidence: 99%

“…Propionibacteria species have a slow growth rate, so that adhesion and adaptation constitute the bottleneck of their beneficial effects within the host. Numerous studies showed the capacity of P. acidipropionici and P. freudenreichii to adhere to human and animal intestinal cells [19,36,37], as well as to human and animal intestinal mucus [38,39,40]. Nonetheless, the adhesion rate evaluated in vitro varied between 0.03 to about 40%, depending on many factors as adhesion model used (cells or mucus), species types, strain type and growth or vehicle medium [19,23,24,38,39,40].…”

Section: Gut Persistence Of Dairy Propionibacteriamentioning

confidence: 99%

Dairy Propionibacteria: Versatile Probiotics

2017

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Dairy propionibacteria are used as cheese ripening starters, as biopreservative and as beneficial additives, in the food industry. The main species, Propionibacterium freudenreichii, is known as GRAS (Generally Recognized As Safe, USA, FDA). In addition to another dairy species, Propionibacterium acidipropionici, they are included in QPS (Qualified Presumption of Safety) list. Additional to their well-known technological application, dairy propionibacteria increasingly attract attention for their promising probiotic properties. The purpose of this review is to summarize the probiotic characteristics of dairy propionibacteria reported by the updated literature. Indeed, they meet the selection criteria for probiotic bacteria, such as the ability to endure digestive stressing conditions and to adhere to intestinal epithelial cells. This is a prerequisite to bacterial persistence within the gut. The reported beneficial effects are ranked according to property’s type: microbiota modulation, immunomodulation, and cancer modulation. The proposed molecular mechanisms are discussed. Dairy propionibacteria are described as producers of nutraceuticals and beneficial metabolites that are responsible for their versatile probiotic attributes include short chain fatty acids (SCFAs), conjugated fatty acids, surface proteins, and 1,4-dihydroxy-2-naphtoic acid (DHNA). These metabolites possess beneficial properties and their production depends on the strain and on the growth medium. The choice of the fermented food matrix may thus determine the probiotic properties of the ingested product. This review approaches dairy propionibacteria, with an interest in both technological abilities and probiotic attributes.

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Screening of Propionibacterium spp. for potential probiotic properties

Cited by 25 publications

References 22 publications

How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria

How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria

Sustainable farming of the mealworm Tenebrio molitor for the production of food and feed

Dairy Propionibacteria: Versatile Probiotics

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