Screening of potential bacterial antagonists for control of sheath blight in rice and development of suitable bacterial formulations for effective application

Kanjanamaneesathian, Mana; Kusonwiriyawong, Chirasak; Pengnoo, Ashara; Nilratana, Ladda

doi:10.1071/ap98022

Cited by 38 publications

(22 citation statements)

References 13 publications

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“…Potato dextrose agar (PDA) was prepared using 200 g of potato, 20 g of dextrose, 17 g of agar and 1 litre of distilled water. B. megaterium and R. solani were obtained from the Laboratory of Biological Control Agent Formulation, Prince of Songkla University, Thailand (Kanjanamaneesathian et al 1998). …”

Section: Methodsmentioning

confidence: 99%

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Spray-dried powder ofBacillus megateriumfor control of rice sheath blight disease: Formulation protocol and efficacy testing in laboratory and greenhouse

Chumthong¹,

Wiwattanapatapee²,

Viernstein³

et al. 2016

Cereal Research Communications

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A spray-dried powder containing Bacillus megaterium was developed and tested for control of rice sheath blight disease in the greenhouse. The formulation consisted of 20 ml of an endospore suspension of B. megaterium, 20% w/v of skim milk powder and 1.25% w/v of polyvinyl pyrrolidone k-90, that were mixed and spray dried at 120 °C. The powder displayed good physical characteristics, such as a low-moisture content and a high solubility in water. Bacterial viability in the powder was 3.5±0.1 × 10 11 cfu/g after production and remained relatively stable (at 2.2±0.1 × 10 10 cfu/g) after 12 months of storage at room temperature. In the laboratory, a 0.1% (w/v) aqueous solution of the formulation was effective in inhibiting the mycelia growth of Rhizoctonia solani (98.5±0.1% inhibition). Under greenhouse conditions, a 0.1% (w/v) aqueous solution applied by either spraying 1 day before inoculating R. solani or spraying 1, 7 and 15 days after inoculation of rice plants with R. solani was more effective in suppressing sheath blight disease than the blank formulation but was less effective than a chemical fungicide control.

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Section: Methodsmentioning

confidence: 99%

“…Soil for growing rice in the greenhouse was prepared as described by Kanjanamaneesathian et al (1998). Soil samples were loaded into plastic pots (21 cm in diameter and 18 cm in height) and the pot was filled with tap water until the soil was soaked.…”

Section: Efficacy Of the Bacterial Powder In Controlling Sheath Blighmentioning

confidence: 99%

Spray-dried powder ofBacillus megateriumfor control of rice sheath blight disease: Formulation protocol and efficacy testing in laboratory and greenhouse

Chumthong¹,

Wiwattanapatapee²,

Viernstein³

et al. 2016

Cereal Research Communications

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“…Phenotypic characterization for identification of strains of B. megaterium is based on morphology, nutrition, growth, and physiological characteristics including utilisation of various substrates [20][21][22][23][24][25]. Large variations and inconsistencies in test results make the identification of this species difficult.…”

Section: Isrn Bacteriologymentioning

confidence: 99%

Rapid Identification of Polyhydroxyalkanoate Accumulating Members of Bacillales Using Internal Primers forphaCGene ofBacillus megaterium

et al. 2013

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Bacillus megaterium is gaining recognition as an experimental model and biotechnologically important microorganism. Recently, descriptions of new strains of B. megaterium and closely related species isolated from diverse habitats have increased. Therefore, its identification requires several tests in combination which is usually time consuming and difficult to do. We propose using the uniqueness of the polyhydroxyalkanoate synthase C gene of B. megaterium in designing primers that amplify the 0.9 kb region of the phaC for its identification. The PCR method was optimized to amplify 0.9 kb region of phaC gene. After optimization of the PCR reaction, two methods were investigated in detail. Method I gave an amplification of a single band of 0.9 kb only in B. megaterium and was demonstrated by several strains of B. megaterium isolated from different habitats. The use of Method I did not result in the amplification of the phaC gene with other members of Bacillales. The specificity for identification of B. megaterium was confirmed using sequencing of amplicon and RT-PCR. Method II showed multiple banding patterns of nonspecific amplicons among polyhydroxyalkanoate accumulating members of Bacillales unique to the respective species. These methods are rapid and specific for the identification of polyhydroxyalkanoate accumulating B. megaterium and members of Bacillales.

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“…Since improved formulations and delivery systems are important for implementation and commercialization of effective biological control (Lewis 1991;Lumsden et al 1995;Larkin et al 1998), we have previously developed a variety of solid formulations, such as floatable granules, floatable pellets, and effervescent fast-disintegrating granules for sheath blight disease control in our laboratory (Kanjanamaneesathian et al 1998Pengnoo et al 2000;Wiwattanapatapee et al 2004Wiwattanapatapee et al , 2007. However, the prototype of these original formulations had in each case undesirable characteristics, such as having a short shelf-life and could be applied only by broadcasting (Kanjanamaneesathian et al 1998;Pengnoo et al 2000;Wiwattanapatapee et al 2004). The comparatively short shelf life was possibly because vegetative cells of the antagonists had been used as the active ingredient in the formulations (Kanjanamaneesathian et al 1998.…”

Section: Introductionmentioning

confidence: 94%

Water-soluble granules containing Bacillus megaterium for biological control of rice sheath blight: formulation, bacterial viability and efficacy testing

Chumthong

Kanjanamaneesathian

Pengnoo

et al. 2008

World J Microbiol Biotechnol

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Endospores of B. megaterium were formulated in granule formulations with sodium alginate, lactose and polyvinylpyrrolidone (PVP K-30) by the wet granulation technique. The granule formulation exhibited good physical characteristics, such as high-water solubility and optimal viscosity, that would be suitable for spray application. The bacteria remained viable in the dry granule formulation at 10 9 c.f.u./g after 24 months storage at room temperature. Under laboratory conditions, aqueous solutions of the formulation showed high activity against mycelial growth of R. solani (99.64 ± 0.14% mycelial inhibition). High viability of the bacterial antagonist on leaf sheath and leaf blade at day 7 after spraying with the formulation was observed (approximately 10 6 c.f.u./g of plant). Application of an equivalent number of un-formulated endospores resulted in much loss of the bacterial endospores even 1 day after application. In a small pilot field study, an aqueous solution of the formulation (3%w/v) applied by spraying at days 1, 5 and 10 after pathogen inoculation of the rice plants was more effective in suppressing rice sheath blight disease than one application of a fungicide (Iprodione) at day 1. Additionally, rice plants sprayed with the aqueous solution of the granule formulation had higher panicle and whole kernel weights than those of fungicide-treated and control (untreated) plants.

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Screening of potential bacterial antagonists for control of sheath blight in rice and development of suitable bacterial formulations for effective application

Cited by 38 publications

References 13 publications

Spray-dried powder ofBacillus megateriumfor control of rice sheath blight disease: Formulation protocol and efficacy testing in laboratory and greenhouse

Spray-dried powder ofBacillus megateriumfor control of rice sheath blight disease: Formulation protocol and efficacy testing in laboratory and greenhouse

Rapid Identification of Polyhydroxyalkanoate Accumulating Members of Bacillales Using Internal Primers forphaCGene ofBacillus megaterium

Water-soluble granules containing Bacillus megaterium for biological control of rice sheath blight: formulation, bacterial viability and efficacy testing

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