Screening of plant-based natural compounds as an inhibitor of FtsZ from Salmonella Typhi using the computational, biochemical and in vitro cell-based studies

Naz, Farah; Kumar, Mukesh; Koley, Tirthankar; Sharma, Priyanka; Haque, Muhammad Anzarul; Kapil, Arti; Kumar, Manoj; Kaur, Punit; Ethayathulla, A.S.

doi:10.1016/j.ijbiomac.2022.07.241

Cited by 16 publications

(6 citation statements)

References 45 publications

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“…On the one hand, the main components of SCEO are trans-anethole and trans-cinnamaldehyde, both of which have satisfactory antibacterial activity [ 15 , 20 ]. The antibacterial mechanism of trans-cinnamaldehyde mainly includes the following: (1) trans-cinnamaldehyde can disturb bacterial cell membrane structures, increase membrane permeability, and finally lead to the leakage of intracellular constituents [ 21 ]; (2) trans-cinnamaldehyde can inhibit membrane-bound ATPases of bacteria, disturb the proton motive force, block ATP synthesis, and finally lead to a reduction in bacterial energy sources [ 22 ]; (3) bacterial cell division is regulated by prokaryotic homologue filamentous temperature-sensitive protein Z (FtsZ), and trans-cinnamaldehyde can interfere with GTP to polymerize FtsZ into Z ring, which finally leads to inhibition of bacterial cell division [ 23 ]; (4) trans-cinnamaldehyde can reduce the expression of luxR and bcsA genes involved in the quorum sensing (QS) system and inhibit the synthesis of autoinducers (AIs) of the QS system, finally inhibiting the formation of biofilms by inhibiting the QS system [ 22 ]. The antibacterial mechanism of trans-anethole may be that trans-anethole disturbs the cell structure of bacteria, causing the leakage of intracellular constituents and the disorder of its anabolism, which finally leads to bacterial death [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

The Use of Star Anise-Cinnamon Essential Oil as an Alternative Antibiotic in Prevention of Salmonella Infections in Yellow Chickens

Chen

et al. 2022

Antibiotics

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Salmonella is capable of harming human and animal health, and its multidrug resistance (MDR) has always been a public health problem. In addition, antibiotic-free or antibiotic-reduced policies have been implemented in poultry production. Therefore, the search for antibiotic alternatives is more urgent than ever before. The aim of this study was to assess the antibacterial activity of star anise-cinnamon essential oil (SCEO) in vitro and its prophylactic effect against the infections of Salmonella pullorum, Salmonella give, and Salmonella kentucky in vivo. The results demonstrated that SCEO is effective against Salmonella pullorum, Salmonella give, and Salmonella kentucky in vitro. Supplementation with SCEO could significantly decrease the infections of Salmonella pullorum and Salmonella give, whereas it could slightly but not significantly decrease the infection of Salmonella kentucky, while also significantly alleviating the body weight (BW) loss caused by the infections of Salmonella pullorum, Salmonella give, and Salmonella kentucky in Yellow chickens. The SCEO had the best prophylactic effect against the infection of Salmonella give in Yellow chickens, followed by the infection of Salmonella pullorum and the infection of Salmonella kentucky. The SCEO, used as an antibiotic alternative, could be an effective prevention strategy against the infections of Salmonella pullorum, Salmonella give, and Salmonella kentucky in Yellow chickens.

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Section: Discussionmentioning

confidence: 99%

The Use of Star Anise-Cinnamon Essential Oil as an Alternative Antibiotic in Prevention of Salmonella Infections in Yellow Chickens

Chen

et al. 2022

Antibiotics

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“…In addition, cinnamaldehyde can inhibit the hydrolysis of GTP and bind to FtsZ, as well as interfere with the formation of z-loop of cell dynamics, thus showing antibacterial activity against bacteria [ 30 ]. In the latest research, the researchers used calculations, biochemistry, and in-vivo-based assays to verify that cinnamaldehyde is a potential inhibitor of S. typhimurium (stFtsZ), and its inhibition rate of stFtsZ GTPase activity and polymerization is up to 70% [ 31 ].…”

Section: Terpenoidsmentioning

confidence: 99%

Biosynthesis Investigations of Terpenoid, Alkaloid, and Flavonoid Antimicrobial Agents Derived from Medicinal Plants

et al. 2022

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The overuse of antibiotics in the past decades has led to the emergence of a large number of drug-resistant microorganisms. In recent years, the infection rate caused by multidrug-resistant microorganisms has been increasing, which has become one of the most challenging problems in modern medicine. Plant-derived secondary metabolites and their derivatives have been identified to display significant antimicrobial abilities with good tolerance and less adverse side effects, potentially having different action mechanisms with antibiotics of microbial origin. Thus, these phyto-antimicrobials have a good prospect in the treatment of multidrug-resistant microorganisms. Terpenoids, alkaloids, and flavonoids made up the predominant part of the currently reported phytochemicals with antimicrobial activities. Synthetic biology research around these compounds is one of the hotspot fields in recent years, which not only has illuminated the biosynthesis pathways of these phyto-antimicrobials but has also offered new methods for their production. In this review, we discuss the biosynthesis investigations of terpenoid, alkaloid, and flavonoid antimicrobial agents—using artemisinin and oleanolic acid (terpenoids), berberine and colchicine (alkaloids), and baicalin (flavonoids) as examples—around their antimicrobial action mechanisms, biosynthesis pathway elucidation, key enzyme identification, and heterologous production, in order to provide useful hints for plant-derived antimicrobial agent discovery and development.

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“…21 Resveratrol can inhibit the formation of the Z ring in Escherichia coli, 22 while scopoletin and daphnetin have also been proven to inhibit the function of FtsZ, thus inhibiting the initial step of cell division. 23,24 It can be seen that FtsZ, along with other proteins associated with bacterial division, represents an important target for antimicrobial agents. 25 Due to the filamentation phenomenon observed in Psa after treatment with ST-F, we hypothesize that ST-F can inhibit the normal proliferation of Psa by targeting division-related proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Citri by targeting chromosome segregation and cell division mechanisms . Resveratrol can inhibit the formation of the Z ring in Escherichia coli, while scopoletin and daphnetin have also been proven to inhibit the function of FtsZ, thus inhibiting the initial step of cell division. , It can be seen that FtsZ, along with other proteins associated with bacterial division, represents an important target for antimicrobial agents …”

Section: Introductionmentioning

confidence: 99%

Streptothricin-F Inhibition of FtsZ Function: A Promising Approach for Controlling Pseudomonas syringae pv. actinidiae

Wang,

Mi,

Mao

et al. 2024

J. Agric. Food Chem.

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Pseudomonas syringae pv. actinidiae (Psa) is a significant pathogenic bacterium affecting the kiwifruit industry. This study investigated the target sites of streptothricin-F (ST-F), produced by Streptomyces lavendulae gCLA4. The inhibition of ST-F on Psa was examined by the microscopic structural differences of Psa before and after treatment with ST-F, as well as the interaction between ST-F and cell division-related proteins. The results revealed filamentation of Psa after ST-F treatment, and fluorescence microscopy showed that ST-F inhibited the formation of the Z-ring composed of FtsZ protein. In vitro experiments and molecular docking demonstrated that ST-F can bind to FtsZ with a binding energy of 0.4 μM and inhibit FtsZ's GTP-dependent polymerization reaction. In addition, ST-F does not exert inhibitory effects on cell division in Psa strains overexpressing f tsZ. In conclusion, FtsZ is one of the target sites for ST-F inhibition of Psa, highlighting its potential as a therapeutic target for controlling Psa-induced kiwifruit bacterial canker.

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Screening of plant-based natural compounds as an inhibitor of FtsZ from Salmonella Typhi using the computational, biochemical and in vitro cell-based studies

Cited by 16 publications

References 45 publications

The Use of Star Anise-Cinnamon Essential Oil as an Alternative Antibiotic in Prevention of Salmonella Infections in Yellow Chickens

The Use of Star Anise-Cinnamon Essential Oil as an Alternative Antibiotic in Prevention of Salmonella Infections in Yellow Chickens

Biosynthesis Investigations of Terpenoid, Alkaloid, and Flavonoid Antimicrobial Agents Derived from Medicinal Plants

Streptothricin-F Inhibition of FtsZ Function: A Promising Approach for Controlling Pseudomonas syringae pv. actinidiae

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