Screening of Phosphate Solubilization Identifies Six Pseudomonas Species with Contrasting Phytostimulation Properties in Arabidopsis Seedlings

López-Hernández, José; García‐Cárdenas, Elizabeth; López‐Bucio, Jesús Salvador; Jiménez‐Vázquez, Kirán Rubí; Cruz, Homero Reyes de la; Ferrera-Rodríguez, Ofelia; Santos-Rodríguez, Dulce Lizbeth; Ortíz-Castro, Randy; López‐Bucio, José

doi:10.1007/s00248-022-02080-y

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…The 16S rRNA gene sequencing revealed RR1 as (Bacillus velezensis), NG4 as (Bacillus cereus), ASC1 as (Pseudomonas baetica), and AFS3 as (Staphylococcus pasteuri). The role of Pseudomonas baetica (González et al, 2021), Staphylococcus pasteuri (Bhattacharyya et al, 2020), Bacillus velezensis (López-Hernández et al, 2022), and Bacillus cereus (Akhtar et al, 2021) has been reported in earlier studies as PGPR with multifarious PGP activities and our observations are in line with these reports. The current study exhibited the diversity of indigenous PGPR from the Kumaon Himalayas.…”

Section: Discussionsupporting

confidence: 93%

“…ASC1 was the highest P solubilizer among all, followed by RR1. Other similar studies of P solubilization have also been reported (Wang et al, 2020;López-Hernández et al, 2022). Additionally, all four selected isolates were noted to produce siderophores up to variable levels.…”

Section: Discussionsupporting

confidence: 75%

“…Production of siderophores, which aids in iron acquisition and accessibility for plants. This PGPR-triggered iron transport helps chlorophyll buildup and assists with iron retention in the edible sections of plants (López-Hernández et al, 2022). Wheat plants under drought stress were used to examine the ability of isolates AFS3, ASC1, RR1, and NG4 to promote plant growth.…”

Section: Discussionmentioning

confidence: 99%

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Plant-beneficial Bacillus, Pseudomonas, and Staphylococcus spp. from Kumaon Himalayas and their drought tolerance response

Sati

Pande²,

Samant³

2023

Front. Sustain. Food Syst.

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Plant growth-promoting rhizobacteria (PGPR) have been shown to augment plant responses against drought and other abiotic stresses. In the present study, we isolated 27 bacteria from the rhizosphere of various plants cultivated in the Kumaon Himalayas., and to measure their abiotic stress tolerance, these 27 isolates were subjected to variations in pH, temperature, and drought. All 27 isolates were also screened for various plant growth-promoting traits. Among these, the four isolates RR1, ASC1, AFS3, and NG4 demonstrated various plant growth promotion activities including the synthesis of indole-3-acetic acid (IAA), siderophores, ammonia, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production, and concomitantly high tolerance to abiotic stresses. Moreover, 16S rRNA sequencing of these four isolates validated their identities as Bacillus, Pseudomonas, and Staphylococcus sp. Finally, to assess the in-vivo drought tolerance potential of these four isolates, a pot-trial experiment was undertaken in wheat cultivar VL-892. The results demonstrated that inoculating wheat plants with these four PGPR isolates greatly improved plant growth under drought circumstances by increasing root and shoot length and both fresh and dry weight of root and shoot. This study endeavors to discover the biochemical and molecular diversity of cultivable PGPR in six remotely located districts of Uttarakhand. In conclusion, the drought-tolerant PGPR strains described in this study are plant-beneficial and can effectively mobilize nutrients under drought conditions. Consequently, they could be used as bioinoculants to alleviate drought stress in wheat plants, in a sustainable manner. To the best of our knowledge, this is the first report of exploring the diversity and characterization of PGPR from the Kumaon Himalayas and their drought evaluation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Plant-beneficial Bacillus, Pseudomonas, and Staphylococcus spp. from Kumaon Himalayas and their drought tolerance response

Sati

Pande²,

Samant³

2023

Front. Sustain. Food Syst.

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“…Ahmad et al [48] reported that cotton seeds inoculated with Bacillus strains having the ability to solubilize P and Zn significantly promoted growth attributes over uninoculated control. PGPRs such as Staphylococcus pasteuri, Bacillus cereus, and Bacillus velezensis have been reported in earlier studies as promising mineral solubilizers with multifarious plant growth-promoting activities [92][93][94]. Among these plant growth activities, production of IAA, siderophores, biofilm formation, and ACC-deaminase activities play important roles in promoting plant growth, which might be true in the current study.…”

Section: Discussionmentioning

confidence: 56%

Mineral Solubilizing Rhizobacterial Strains Mediated Biostimulation of Rhodes Grass Seedlings

Javaid,

Mushtaq,

Mumtaz

et al. 2023

Microorganisms

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Minerals play a dynamic role in plant growth and development. However, most of these mineral nutrients are unavailable to plants due to their presence in fixed forms, which causes significant losses in crop production. An effective strategy to overcome this challenge is using mineral solubilizing bacteria, which can convert insoluble forms of minerals into soluble ones that plants can quickly assimilate, thus enhancing their availability in nutrient-depleted soils. The main objective of the present study was to isolate and characterize mineral solubilizing rhizobacteria and to assess their plant growth-promoting potential for Rhodes grass. Twenty-five rhizobacterial strains were isolated on a nutrient agar medium. They were characterized for solubilization of insoluble minerals (phosphate, potassium, zinc, and manganese), indole acetic acid production, enzymatic activities, and various morphological traits. The selected strains were also evaluated for their potential to promote the growth of Rhodes grass seedlings. Among tested strains, eight strains demonstrated strong qualitative and quantitative solubilization of insoluble phosphate. Strain MS2 reported the highest phosphate solubilization index, phosphate solubilization efficiency, available phosphorus concentration, and reduction in medium pH. Among tested strains, 75% were positive for zinc and manganese solubilization, and 37.5% were positive for potassium solubilization. Strain MS2 demonstrated the highest quantitative manganese solubilization, while strains MS7 and SM4 reported the highest solubilization of zinc and potassium through acidifying their respective media. The strain SM4 demonstrated the most increased IAA production in the presence and absence of L-tryptophan. The majority of strains were positive for various enzymes, including urease, catalase protease, and amylase activities. However, these strains were negative for coagulase activity except strains SM7 and MS7. Based on 16S rRNA gene sequencing, six strains, namely, SM2, SM4, SM5, MS1, MS2, and MS4, were identified as Bacillus cereus, while strains SM7 and MS7 were identified as Staphylococcus saprophyticus and Staphylococcus haemolyticus. These strains significantly improved growth attributes of Rhodes grass, such as root length, shoot length, and root and shoot fresh and dry biomasses compared to the uninoculated control group. The present study highlights the significance of mineral solubilizing and enzyme-producing rhizobacterial strains as potential bioinoculants to enhance Rhodes grass growth under mineral-deficient conditions sustainably.

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“…After the removal of the solution, 7% NaOH was added to cover the seedlings and maintained at room temperature for 30 min. The solution was discarded and the seedlings were washed with 40%, 20%, and 10% (v/v) ethanol for 30 min each, and finally stored with 50% glycerol (v/v) at 4 °C [ 26 ]. Plant tissues were fixed on glass slides with 50% (v/v) glycerol and representative images were taken using Nomarski optics in a Leica DM5000B microscope at 20× magnifications.…”

Section: Methodsmentioning

confidence: 99%

Bacillus Strains as Effective Biocontrol Agents Against Phytopathogenic Bacteria and Promoters of Plant Growth

Ramírez-Pool,

Calderón-Pérez,

Ruiz-Medrano

et al. 2024

Microb Ecol

Self Cite

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Modern crop production relies on the application of chemical pesticides and fertilizers causing environmental and economic challenges. In response, less environmentally impactful alternatives have emerged such as the use of beneficial microorganisms. These microorganisms, particularly plant growth-promoting bacteria (PGPB), have demonstrated their ability to enhance plant growth, protect against various stresses, and reduce the need for chemical inputs. Among the PGPB, Bacillus species have garnered attention due to their adaptability and commercial potential. Recent reports have highlighted Bacillus strains as biocontrol agents against phytopathogenic bacteria while concurrently promoting plant growth. We also examined Bacillus plant growth-promoting abilities in Arabidopsis thaliana seedlings. In this study, we assessed the potential of various Bacillus strains to control diverse phytopathogenic bacteria and inhibit quorum sensing using Chromobacterium violaceum as a model system. In conclusion, our results suggest that bacteria of the genus Bacillus hold significant potential for biotechnological applications. This includes developments aimed at reducing agrochemical use, promoting sustainable agriculture, and enhancing crop yield and protection.

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Screening of Phosphate Solubilization Identifies Six Pseudomonas Species with Contrasting Phytostimulation Properties in Arabidopsis Seedlings

Cited by 8 publications

References 44 publications

Plant-beneficial Bacillus, Pseudomonas, and Staphylococcus spp. from Kumaon Himalayas and their drought tolerance response

Plant-beneficial Bacillus, Pseudomonas, and Staphylococcus spp. from Kumaon Himalayas and their drought tolerance response

Mineral Solubilizing Rhizobacterial Strains Mediated Biostimulation of Rhodes Grass Seedlings

Bacillus Strains as Effective Biocontrol Agents Against Phytopathogenic Bacteria and Promoters of Plant Growth

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