Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation

Liao, Wang; Chen, Yisheng; ZhiYang, Ye; Hellmann, Bernhard; Xu, Xueming; Jin, Zhengyu; Ma, Qianli; Yang, Na; Wu, Fengfeng; Jin, Yamei

doi:10.1007/s12161-018-1295-x

Cited by 9 publications

(2 citation statements)

References 33 publications

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“…The electron delocalization pattern supplied stability and a deep blue look. After introducing DPPH compounds to hydrogen-donating agents, the random electron of the nitrogen atom was oxidized to the corresponding hydrazine, resulting in a color turning from dark violet into bright yellow [27]. This DPPH assay, dependent on stoichiometric decolorization, was a well-established technique for detecting antioxidants precisely.…”

Section: Discussionmentioning

confidence: 99%

High‐performance thin‐layer chromatography a densitometric detection of multi‐class bioactive compounds from three species of marine algae Padina and identification of antioxidant substance with mass spectrometry

Hakim

Patel

2022

Separation Science Plus

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Padina is rolled-blade-shaped marine brown algae that often have overgrown and has been well explored for its medicinal qualities. The various species of Padina release a wide range of promising chemical components as part of their chemical defense systems. In this context, a finding was conducted to explore the bioactive compounds using exceptionally sophisticated analytical techniques, such as high-performance thin-layer chromatography. Microchemical and biochemical derivatization was used in the investigation to confirm multi-class bioactive molecules and the antioxidant activity of the separated band. Microchemical derivatization ascertained the appearance of miscellaneous lipophilic substances, essential oils, volatile compounds, coumarin, and bitter principles. To determine the chemical standard of these algae, the qualitative (microchemical) screening of phytochemicals associated with flexible high-performance thin-layer chromatography was utilized. Moreover, biochemical derivatization, which generates a spectacular white band with a purple background to appear on the chromatogram, substantiates the antioxidant activity of a particular band.These results proffer a chromatographic profile of the antioxidant capability of three species of Padina's bioactive components, which will create the necessary information for the standardization and identification of marine algae. Mass spectrometry results revealed the presence of phytol compound from the diterpenoid class, which has antioxidant potential.

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Section: Discussionmentioning

confidence: 99%

High‐performance thin‐layer chromatography a densitometric detection of multi‐class bioactive compounds from three species of marine algae Padina and identification of antioxidant substance with mass spectrometry

Hakim

Patel

2022

Separation Science Plus

View full text Add to dashboard Cite

show abstract

“…All these methods have been shown to be able to analyze different components of edible oil in previous studies [ 13 ]. GC and GC-MS are standardized techniques for the identification and quantification of fatty acids, volatiles, and phytosterols [ 14 ]. HPLC is beneficial for the identification and quantification of carotenoids, tocopherols, and polyphenols, while fatty acids and volatile substances can also be evaluated using IR [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Combination of fingerprint and chemometric analytical approaches to identify the geographical origin of Qinghai-Tibet plateau rapeseed oil

Ye,

Wang,

Gan

et al. 2024

Heliyon

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Development and validation of an HPTLC–DPPH assay and its application to the analysis of honey

Islam

Sostaric

Lim

et al. 2020

JPC-J Planar Chromat

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Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation

Cited by 9 publications

References 33 publications

High‐performance thin‐layer chromatography a densitometric detection of multi‐class bioactive compounds from three species of marine algae Padina and identification of antioxidant substance with mass spectrometry

High‐performance thin‐layer chromatography a densitometric detection of multi‐class bioactive compounds from three species of marine algae Padina and identification of antioxidant substance with mass spectrometry

Combination of fingerprint and chemometric analytical approaches to identify the geographical origin of Qinghai-Tibet plateau rapeseed oil

Development and validation of an HPTLC–DPPH assay and its application to the analysis of honey

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