Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source

Teeka, Jantima; Imai, Tsuyoshi; Cheng, Xuehang; Reungsang, Alissara; Higuchi, Takaya; Yamamoto, Kōichi; Sekine, Masahiko

doi:10.2965/jwet.2010.373

Cited by 23 publications

(17 citation statements)

References 26 publications

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“…A total of 120 isolates showed black-blue coloration when stained with Sudan black B, a preliminary screening agent for lipophilic compounds, and a total of 62 isolates showed positive result with Nile blue A staining, a specific dye for the presence of PHA granules. Teeka et al [29] used this method to screen the potential PHA-producing bacteria from soil, and Ramachandra and Abdullah [30] also observed the colonies formed on nutrient-rich medium under ultraviolet light (UV) to screen for the pink fluorescence which indicated the presence of PHA producers. Kitamura and Doi [31] first demonstrated this viable colony method on agar plates; they induced the isolates to accumulate PHA by culturing in E 2 medium, containing 2% (w/v) glucose before Nile blue A staining.…”

Section: Resultsmentioning

confidence: 99%

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bhuwal

Singh

Aggarwal

et al. 2014

Bioresour. Bioprocess.

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Background: In the present study, we aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent. Results: A total of 120 isolates were isolated from different soil samples and a total of 62 isolates showed positive results with Nile blue A staining, a specific dye for PHB granules and 27 isolates produced PHB using cardboard industry effluent. The selected isolate NA10 was identified as Bacillus sp. NA10 by studying its morphological, biochemical, and molecular characteristics. The growth pattern for the microorganism was studied by logistic model and exactly fitted in the model. A maximum cell dry weight (CDW) of 7.8 g l −1 with a PHB concentration of 5.202 g l −1 was obtained when batch cultivation was conducted at 37°C for 72 h, and the PHB content was up to 66.6% and productivity was 0.072 g l −1 h −1 in 2.0 L fermentor. Chemical characterization of the extracted PHB was done by H 1 NMR, Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), Gas chromatography-mass spectrometry (GC-MS) analysis to determine the structure, melting point, and molecular mass of the purified PHB. The polymer sheet of extracted polymer was prepared by blending the polymer with starch for packaging applications. Conclusions: The isolate NA10 can be a good candidate for industrial production of PHB from cardboard industry waste water cost-effectively and ecofriendly.

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Section: Resultsmentioning

confidence: 99%

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bhuwal

Singh

Aggarwal

et al. 2014

Bioresour. Bioprocess.

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“…B. cepacia grown on crude glycerol generated by a small batch biodiesel processor at SUNY-ESF containing free fatty acids as a carbon source for comparable growth, yet much lower glycerol consumption, indicating that B. cepacia metabolized fatty acids, along with glycerol, for bacterial growth and PHA production. 88 Teeka et al 96 isolated a previously unidentified strain, AIK7, which also demonstrated enhanced bacterial growth, even improved PHA yield, when using waste glycerol containing free fatty acid instead of pure glycerol.…”

Section: Production and Characterization Of Polyhydrox-yalkanoates Frmentioning

confidence: 99%

Bioplastics from waste glycerol derived from biodiesel industry

Zhu

Chiu

Nakas

et al. 2013

J of Applied Polymer Sci

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Polyhydroxyalkanoates (PHAs) are polyesters that can be biologically synthesized by many microorganisms and engineered plants that have been investigated by microbiologists, biochemists, polymer scientists, material engineers, and medical researchers for several decades. Research on microbial production of PHAs has been extensively focused on using pure carbon sources, such as sugars and fatty acids. Practical considerations of production costs of PHAs have resulted in research efforts to use alternative renewable and inexpensive feedstocks. One potential feedstock for the production of PHA polymers is the glycerol waste byproduct of biodiesel production. The major focus of this review is the production of PHA polymers from glycerol. A review of biosynthetic pathways for PHAs production from glycerol, current production of waste glycerol in biodiesel industry, physical and mechanical properties of PHAs, and applications of PHAs in the areas of packaging industry, implant materials, drug carrier, biofuels, are covered.

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“…The MSM supplemented with 2% (w/v) CG contained 1.44 g l -1 of total nitrogen, 1.28 g l -1 of total phosphorus, 27.5 g l -1 chemical oxygen demand (COD), and 1.384 g l -1 of sodium element [33]. It appeared that highest PHAs content of 45% cell dry weight (CDW) was achieved in 72 h ( Fig.…”

Section: Phas Biosynthesismentioning

confidence: 89%

“…Aerobic condition was maintained by shaking at 30°C and 150 rpm. PHAs and glycerol determination were described in Teeka et al [33].…”

Section: Batch Cultivationmentioning

confidence: 99%

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Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

Teeka

Imai

Reungsang

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Biodiesel-contaminated wastewater was used to screen for PHAs-producing bacteria by using crude glycerol as the sole carbon source. A gram-negative THA_AIK7 isolate was chosen as a potential PHAs producer. The 16S rRNA phylogeny indicated that THA_AIK7 isolate is a member of Novosphingobium genus which is supported by a bootstrap percentage of 100% with Novosphingobium capsulatum. The 1,487 bp of 16S rRNA gene sequence of THA_AIK7 isolate has been deposited in the GenBank database under the accession number HM031593. Polymer content of 45% cell dry weight was achieved in 72 h with maximum product yield coefficient of 0.29 g PHAs g⁻¹ glycerol. Transmission electron micrograph results exhibited the PHAs granules accumulated inside the bacterial cell. PHAs polymer production in mineral salt media supplemented with 2% (w/v) of crude glycerol at initial pH 7 was extracted by the sodium hypochlorite method. Polymer film spectrographs from Nuclear magnetic resonance displayed a pattern of signal virtually identical to spectra of commercial PHB. Thermal analysis by Differential scanning calorimeter showed a melting temperature at 179°C. Molecular weight analysis by Gel permeation chromatography showed two main peaks of 133,000 and 700 g mol⁻¹ with weight-average molecular weight value of 23,800 and number-average molecular weight value of 755. Endotoxinfree of PHAs polymer was preliminarily assessed by a negative result of the gel-clot formation, Pyrotell® Single test vial, at sensitivity of 0.25 EU ml⁻¹. To our knowledge, this is the first reported test of endotoxin-free PHAs naturally produced from gram-negative bacteria which could be used for biomedical application.

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Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source

Cited by 23 publications

References 26 publications

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Poly-β-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10

Bioplastics from waste glycerol derived from biodiesel industry

Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

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