Screening of pH-responsive long-circulating polysaccharide–drug conjugate nanocarriers for antitumor applications

Zhang, Xinyu; Li, Dandan; Huang, Jun; Ou, Kunyong; Yan, Binyuan; Shi, Fang; Zhang, Jiayuan; Zhang, Junfu; Pang, Jun; Kang, Yang; Wu, Jun

doi:10.1039/c8tb02474j

Cited by 43 publications

(7 citation statements)

References 44 publications

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“…Construction and Characterization of M1HD@RPR. Exploiting pH sensitivity in tumor sites is an effective strategy for targeted delivery of antitumor drugs [34][35][36]. Here, we created a pH-sensitive DOX by Schiff base reaction between DOX and HA (HA-DOX).…”

Section: Resultsmentioning

confidence: 99%

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy

Yang

Zhou

et al. 2022

Research

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Improving the efficacy of melanoma treatment remains an important global challenge. Here, we combined chemotherapy with protein tyrosine phosphatase nonreceptor type 2(Ptpn2) based immunotherapy in an effort to address this challenge. Short-hairpin RNA (shRNA) targeting Ptpn2 was coencapsulated with doxorubicin (DOX) in the cell membrane of M1 macrophages (M1HD@RPR). The prepared nanoparticles (NPs) were effectively phagocytosed by B16F10 cells and M1 macrophages, but not by M0 macrophages. Hence, NP evasion from the reticuloendothelial system (RES) was improved and NP enrichment in tumor sites increased. M1HD@RPR can directly kill tumor cells and stimulate immunogenic cell death (ICD) by DOX and downregulate Ptpn2. It can promote M1 macrophage polarization and dendritic cell maturation and increase the proportion of CD8+ T cells. M1HD@RPR killed and inhibited the growth of primary melanoma and lung metastatic tumor cells without harming the surrounding tissue. These findings establish M1HD@RPR as a safe multifunctional nanoparticle capable of effectively combining chemotherapy and gene immunotherapies against melanoma.

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Section: Resultsmentioning

confidence: 99%

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy

Yang

Zhou

et al. 2022

Research

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“…75 Dynamic covalent chemistry such as imine linkage, oxime linkage, hydrazone linkage, disulfide exchange, and boronic ester chemistry are also utilized to synthesize HA-based nanoparticles. [76][77][78][79] As HA consists of glucuronic acid and N-acetyl glucosamine repeat units with abundant carboxylate and hydroxy groups, these functional groups were easily modified to develop bioactive nanomaterials and hydrogels. 80 The hydroxyls at the C2 and C3 positions of the glucuronic acid ring in HA can be oxidized using sodium periodate to produce two aldehyde groups per repeating unit of HA.…”

Section: Ha Nanoparticlesmentioning

confidence: 99%

Section: Ha Nanoparticlesmentioning

confidence: 99%

Dynamic covalent crosslinked hyaluronic acid hydrogels and nanomaterials for biomedical applications

et al. 2022

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“…In particular, the SOFs with well-defined pores have great potential in adsorbing and releasing drugs (Tian et al., 2016 , 2017 ; Yao et al., 2017 ). In order to achieve efficient and controlled release, various stimuli-responsive (such as pH) techniques have been developed in the past decade (Zhang et al., 2019a , 2019b , 2020 ; Zhu et al., 2020 ; Li et al., 2021c ). Therefore, it is of great scientific and clinical interest to explore new responsive SOFs for the construction of DDSs.…”

Section: Introductionmentioning

confidence: 99%

A pH-responsive complex based on supramolecular organic framework for drug-resistant breast cancer therapy

Zhang¹,

Zeng²,

Ma³

et al. 2021

Drug Delivery

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Chemotherapy is one of the main ways to treat breast cancer clinically. However, the multidrug resistance to anti-tumor drugs limits their clinical use. To overcome these drawbacks, development of drug delivery systems (DDSs) has attracted more and more attention in cancer therapy. At present, the preparation and purification process are complicated for many reported DDSs, while clinic calls for new DDSs that are more convenient for preparation. Here, a new pH-responsive supramolecular organic framework drug delivery complex loading doxorubicin (DOX) is fabricated. Anti-tumor activity of the system in vitro was investigated by cell cytotoxicity, uptake assay, and cell apoptosis analysis. The anti-tumor activity in vivo was investigated by inspecting nude mice body weight, tumor volume, and weight, also a preliminary mechanism probe was conducted by HE and TUNEL staining. The DOX@SOF displayed high stability, good biocompatibility, and pH regulated drug release. At acid condition, the hydrazone bonds would be broken, which result in the dissociation of SOF, and then the drugs would be released from the system. Furthermore, DOX@SOF enhanced cellular internalization. Both in vitro and in vivo experiments reflected that DOX@SOF could enhance the anti-tumor activity of DOX for the MCF-7/ADR tumor cells and tumors. This study provides a highly efficient strategy to prepare stimulus-responsive supramolecular drug delivery complex for treatment of drug-resistant cancer, the results presented inspiring scientific interests in exploring new drug delivery strategy and reversing multi-drug resistance for clinical chemotherapy.

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Screening of pH-responsive long-circulating polysaccharide–drug conjugate nanocarriers for antitumor applications

Abstract: Schematic illustration of the development of long-circulating pH-responsive polysaccharide–DOX prodrug nanoparticles for antitumor applications.

Cited by 43 publications

References 44 publications

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy

Macrophage Membrane-Camouflaged shRNA and Doxorubicin: A pH-Dependent Release System for Melanoma Chemo-Immunotherapy

Dynamic covalent crosslinked hyaluronic acid hydrogels and nanomaterials for biomedical applications

A pH-responsive complex based on supramolecular organic framework for drug-resistant breast cancer therapy

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