Screening of PGPR from the Rhizosphere of Groundnut (Arachis hypogea): Characterization and Application

Sulthana, R. Nigar; Rajanikanth, A.; Padamavathi, M.

doi:10.20546/ijcmas.2018.707.486

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…The absorbance measured at 570nm g UV-Visible spectrophotometer. The concentration of solubilized phosphate was calculated using standard curve of KH2PO4 [10,11] .…”

Section: Quantitative Testmentioning

confidence: 99%

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Assessing the potential of phosphate solubilizing bacteria isolated from rhizospheres of Sorghum bicolor grown in Surat, south Gujarat

Bhalani,

Dasgupta

2024

South Asian J. Agric. Sci.

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Phosphate-solubilizing bacteria (PSB) convert insoluble soil phosphates into soluble phosphorus and are potential suppliers of soluble phosphorus to plants. The current study was undertaken to isolate, and screen PSBs from the rhizosphere of Sorghum (s L.) grown in Surat, Gujarat. A total of 21 bacterial strains were isolated. The isolated strains were screened for their phosphate solubilization efficacy using Pikovskaya's agar and broth. Six isolates were found to be phosphate solubilizers. The isolate SB14 showed maximum effectiveness in phosphate solubilization. Morphological and biochemical characterization identified the isolate as Klebsiella sp. Plant growth-promoting traits of the isolated SB14 strain were assessed by a pot culture experiment conducted with groundnut seeds (Arachis hypogaea) using seeds treated with the bioinoculant of the isolated strain; uninoculated seeds were used as controls. Significant enhancement in seed germination of the inoculated seeds was observed. A marked growth in the aerial parts was also recorded.

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“…The absorbance measured at 570nm g UV-Visible spectrophotometer. The concentration of solubilized phosphate was calculated using standard curve of KH2PO4 [10,11] .…”

Section: Quantitative Testmentioning

confidence: 99%

“…This precipitate reacts with amino-naphthol-sulfonic acid, producing a molybdenum complex that turns the solution blue, indicating the presence of phosphorus. This method, known as the molybdenum blue method, is commonly employed for the detection and quantification of phosphorus in sample [11] .…”

Section: Isolation Of the Bacterial Isolatesmentioning

confidence: 99%

Assessing the potential of phosphate solubilizing bacteria isolated from rhizospheres of Sorghum bicolor grown in Surat, south Gujarat

Bhalani,

Dasgupta

2024

South Asian J. Agric. Sci.

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“…A standard curve of KH 2 PO 4 (0-2 ppm) was made against O.D. 600 nm to estimate the soluble [21], Phosphatase activity was measured using pnitrophenol [23,24] and nal pH was determined with a glass electrode [25]. In the ask, one ml of supernatant, four ml of buffer (pH 5), and one ml of lter-sterilized 0.115M p-nitrophenyl phosphate solution were added.…”

Section: Materials and Reagentsmentioning

confidence: 99%

Formation of bio-hybrid nanocomposite [P. oxalicum/P 2 O 5 +Ca 3 (PO 4 ) 2 ] BHC by Penicillium oxalicum and its application on the growth of canola

Shalaby¹,

Attia

Mahmoud

et al. 2022

Preprint

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The novel synthesis of biohybrid nanocomposite [P. oxalicum/P2O5 + Ca3(PO4)2]BHC by Penicillium oxalicum in presence of tricalcium phosphate and phosphorus pentoxide for phosphate rocks. Phosphorus elemental solubilization from insoluble rock phosphate by utilizing microorganisms is a promising approach for lowering production costs and increasing environmental sustainability. P. oxalicum was isolated and identified in NRC, Cairo, Egypt, and proved its ability of P highest phosphate solubilization potential by estimating clear zone and solubilization index in Pikovskaya’s agar medium and available P and phosphatase enzyme in broth media. The highest dry shoot is the inoculation treatment P. oxalicum + Extract (52.59 g) in presence of TCP and the highest dry root is the inoculation treatment P. oxalicum (6.72 g) in presence of RP. The highest phosphate content and uptake are extremely in the presence of rock phosphate and tricalcium phosphate. the results showed that significantly increased the total counts of PSM at all sources of mineral phosphate fertilizer and PSF count is increased in presence of RP and TCP more than SSP compared with control. The biohybrid nanocomposite showed an absorbance of 0.26 and 2.23 at the wavelength (λ) 378 nm and 465 nm. Also, significant changes in the absorption index and indirect/direct bandgap of the biohybrid nanocomposite have been detected. To perform frequency calculations for crystal models and isolated molecules and optimize the molecular structure, the materials Studio 7.0 program using TDDFT/DMol3 and CASTEP methodologies were utilized. The DFT-Gaussian09W-vibration values are quite similar to the experimental data either in the structure or in the optical properties.

show abstract

Screening of PGPR from the Rhizosphere of Groundnut (Arachis hypogea): Characterization and Application

Cited by 2 publications

References 6 publications

Assessing the potential of phosphate solubilizing bacteria isolated from rhizospheres of Sorghum bicolor grown in Surat, south Gujarat

Assessing the potential of phosphate solubilizing bacteria isolated from rhizospheres of Sorghum bicolor grown in Surat, south Gujarat

Formation of bio-hybrid nanocomposite [P. oxalicum/P 2 O 5 +Ca 3 (PO 4 ) 2 ] BHC by Penicillium oxalicum and its application on the growth of canola

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