Screening of Oligonucleotide Aptamers and Application in Detection of Pesticide and Veterinary Drug Residues

Zou, Xue‐Mei; Zhou, Jiawei; Song, Shang-Hong; Chen, Guanhua

doi:10.1016/s1872-2040(19)61153-9

Cited by 14 publications

(5 citation statements)

References 88 publications

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“…The target-bound sequences are amplified by PCR (DNA SELEX) or reverse transcription PCR (RNA SELEX); the products are used for the next selection round, performing the same sequence and target molecule interaction process. After several rounds of selection, the enriched oligonucleotides are sequenced and evaluated for their binding capabilities [63][64][65][66]. However, there are some deficiencies to be overcome; one of them is stringency, as SELEX assumes that the most enriched sequences are the most specific binders, which is not always the case, and high-affinity binders can sometimes be overlooked due to insufficient stringency in selection conditions.…”

Section: Production Of Aptamers As Recognizing Elementsmentioning

confidence: 99%

Aptasensors for the Detection of Environmental Contaminants of High Concern in Water Bodies: A Systematic Review

Reynoso,

Sfragano,

González-Perea

et al. 2024

Chemosensors

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With the advancement of technology and increasing industrial activity, anthropogenic contaminants are currently detected where there is no record of their presence or insufficient information about their toxicological impact. Consequently, there are not sufficiently robust local or global regulations, the ecotoxicological and human health risks are critical, and they may not be routinely monitored despite being ubiquitous. The interest in studying environmental contaminants, including micropollutants and emerging contaminants, in complex environmental water samples has grown in the last decade. Due to the concentrations in which they are typically found in the environment and the rapid global dispersion, the detection procedures for these substances must be capable of measuring very low concentrations. Many efforts have been made to improve remediation procedures or develop novel analytical methods for their determination. Although there are several robust and reliable standard analytical techniques for their monitoring, pollutant contamination requires simple and inexpensive methods for massive, in situ monitoring campaigns. In this regard, biosensors have emerged as devices with high selectivity, sensitivity, easy operation, and short analysis times. Aptasensors are biosensors based on a nucleic acid recognition element (aptamer). Due to their synthetic nature, stability, and easy production, aptamers are frequently employed to develop bioassays. This work presents a systematic review of the trends in using aptasensors for detecting environmental contaminants present in environmental water samples, as well as the estimation of the potential technological contribution these devices might give to environmental monitoring.

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Section: Production Of Aptamers As Recognizing Elementsmentioning

confidence: 99%

Aptasensors for the Detection of Environmental Contaminants of High Concern in Water Bodies: A Systematic Review

Reynoso,

Sfragano,

González-Perea

et al. 2024

Chemosensors

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“…For these reasons, the development of rapid, and portable sensing devices for the detection of pesticides in environmental sources is required. Aptamer-based biosensors for the detection of pesticides and their byproducts have been reviewed (Yan X. et al, 2018;Zou et al, 2019). Recently, several colorimetric and fluorescent biosensors for the detection of pesticides have been described.…”

Section: Pesticides and Insecticidesmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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“…Aptamers are short, single-stranded oligonucleotides (DNA or RNA, usually 20 to 110 nucleotides in length) capable of binding to a specific molecule with an affinity that can be of the same order of magnitude as that of antibodies. An aptamer specific to a target is identified in vitro by an iterative selection process called SELEX (systematic evolution of ligands by exponential enrichment) performed on an initial mixture of a very high number of different oligonucleotides [ 161 , 162 , 163 ]. Most of the already identified specific oligonucleotide sequences are directed against large molecules such as peptides, proteins, nucleic acids and even bacteria, but also for a significant number of small molecules such as drugs, organic pollutants, and even inorganic ions [ 163 , 164 , 165 ].…”

Section: Oligosorbentsmentioning

confidence: 99%

“…An aptamer specific to a target is identified in vitro by an iterative selection process called SELEX (systematic evolution of ligands by exponential enrichment) performed on an initial mixture of a very high number of different oligonucleotides [ 161 , 162 , 163 ]. Most of the already identified specific oligonucleotide sequences are directed against large molecules such as peptides, proteins, nucleic acids and even bacteria, but also for a significant number of small molecules such as drugs, organic pollutants, and even inorganic ions [ 163 , 164 , 165 ]. In comparison with antibodies, aptamers offer several advantages as selective extraction tools.…”

Section: Oligosorbentsmentioning

confidence: 99%

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Delaunay

Combès

Pichon

2020

Toxins

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The evolution of instrumentation in terms of separation and detection allowed a real improvement of the sensitivity and analysis time. However, the analysis of ultra-traces of toxins in complex samples requires often a step of purification and even preconcentration before their chromatographic analysis. Therefore, immunoaffinity sorbents based on specific antibodies thus providing a molecular recognition mechanism appear as powerful tools for the selective extraction of a target molecule and its structural analogs to obtain more reliable and sensitive quantitative analysis in environmental, food or biological matrices. This review focuses on immunosorbents that have proven their efficiency in selectively extracting various types of toxins of various sizes (from small mycotoxins to large proteins) and physicochemical properties. Immunosorbents are now commercially available, and their use has been validated for numerous applications. The wide variety of samples to be analyzed, as well as extraction conditions and their impact on extraction yields, is discussed. In addition, their potential for purification and thus suppression of matrix effects, responsible for quantification problems especially in mass spectrometry, is presented. Due to their similar properties, molecularly imprinted polymers and aptamer-based sorbents that appear to be an interesting alternative to antibodies are also briefly addressed by comparing their potential with that of immunosorbents.

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Screening of Oligonucleotide Aptamers and Application in Detection of Pesticide and Veterinary Drug Residues

Cited by 14 publications

References 88 publications

Aptasensors for the Detection of Environmental Contaminants of High Concern in Water Bodies: A Systematic Review

Aptasensors for the Detection of Environmental Contaminants of High Concern in Water Bodies: A Systematic Review

Aptamer-Based Biosensors for Environmental Monitoring

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

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