Screening of Oleaginous Yeast Strains Tolerant to Lignocellulose Degradation Compounds

Abstract

High cost of triacylglycerol lipid feedstock is the major barrier for commercial production of biodiesel. The fermentation of oleaginous yeasts for lipid production using lignocellulose biomass provides a practical option with high economic competitiveness. In this paper, the typical oleaginous yeast strains were screened under the pressure of lignocellulose degradation compounds for selection of the optimal strains tolerant to lignocellulose. The inhibitory effect of lignocellulose degradation products on the oleaginous yeast fermentation was carefully investigated. Preliminary screening was carried out in the minimum nutritious medium without adding any expensive complex ingredients then was carried out in the lignocellulosic hydrolysate pretreated by dilute sulfuric acid. Seven typical lignocellulose degradation products formed in various pretreatment and hydrolysis processing were selected as the model inhibitors, including three organic acids, two furan compounds, and two phenol derivatives. The inhibition of the degradation compounds on the cell growth and lipid productivity of the selected oleaginous yeasts were examined. Acetic acid, formic acid, furfural, and vanillin were found to be the strong inhibitors for the fermentation of oleaginous yeasts, while levulinic acid, 5-hydroxymethylfurfural, and hydroxybenzaldehyde were relatively weak inhibitors. Trichosporon cutaneum 2.1374 was found to be the most adopted strain to the lignocellulose degradation compounds.

Bioprospecção de leveduras oleaginosas do Agreste Pernambucano: síntese de lipídios para a produção de biodiesel a partir do resíduo glicerol bruto

“…O gênero Yarrowia é conhecido na literatura por sua capacidade de acumular lipídios (Chen et al, 2009;Signori et al, 2016). Este estudo comprovou que todos os isolados de Yarrowia coletados nos laticínios da bacia do Agreste-PE apresentaram potencial de crescimento e de acúmulo de lipídios, utilizando o glicerol bruto como fonte de carbono.…”

“…Muitas vantagens estão envolvidas no uso de leveduras oleaginosas para a produção de lipídios, como a alta taxa de crescimento em biorreatores, a capacidade de assimilar diversas fontes de carbono e não serem afetadas por limitações de espaço, clima ou variações de luz (Ami et al, 2014). Entre as leveduras mais citadas na literatura, como oleaginosas, figuram as do gênero Rhodotorula e da espécie Yarrowia lipolytica (Chen et al, 2009;Signori et al, 2016).…”

A Review on The Bioconversion of Lignin to Microbial Lipid with Oleaginous Rhodococcus opacus

“…Oleaginous bacterial accumulation of lipids for biofuel production from industrial waste is being studied extensively [41][42][43][44][45][46][47]. Transcriptomic and proteomic studies have also identified TAG biosynthetic genes and proteins [48][49][50][51][52][53].…”

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

“…Many studies have explored the use of common oleaginous yeast species in single-cell oil (SCO) production (Breuer and Harms, 2006;Chen et al, 2009;Magdouli et al, 2014). Table 1 shows the typical oleaginous yeast species that were used for bioconversion of cellulosic sugars to SCO.…”

“…Using renewable materials as substrates can enhance the economy of the bioconversion process for Y. lipolytica (Chen et al, 2009;Liang and Jiang, 2013). Molasses, N -acetylglucosamine, sewage sludge, palm oil mill, olive oil mill wastewater, whey, municipal wastewater, industrial fats, and saturated spent fat are examples of such materials (Barth and Gaillardin, 1997;Papanikolaou et al, 2003;Fickers et al, 2005;Chen et al, 2009;Thevenieau and Nicaud, 2013).…”