Screening of novel actinobacteria and characterization of the potential isolates from mangrove sediment of south coastal India

Arumugam, T.; Kumar, P. Senthil; Kameshwar, R.; Prapanchana, K.

doi:10.1016/j.micpath.2017.03.035

Cited by 25 publications

(10 citation statements)

References 29 publications

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“…The results showed that among the carbon sources antibacterial and antifungal activity was higher when the isolate was grown in a medium having starch casein agar for lake Chitu isolates enhanced the production of secondary metabolites and the zone of inhibition against reference bacterial and fungal strain which is in agreement with [27][28] that starch casein medium found to be good base for antibacterial and antifungal metabolite production and D-xylose for lake Arenguade in agreement with [29] dextrose prove to be the best carbon source for antibiotic production by S. kanamyceticus M27 with a maximum zone of inhibition (22 mm) and 19 mm respectively. Our result is also supported by [30][31][32][33] that they concluded that the rate at which carbon source is metabolized can in uence the formation of biomass or production of primary or secondary metabolites and fast growth due to high concentrations of rapidly metabolized sugars is often associated with low productivity of secondary metabolites.…”

Section: Discussionsupporting

confidence: 76%

In vitro bioassay of antibacterial and antifungal activity studies of actinomycetes from soda lakes of Ethiopia

Chali

Belay

Bacha

2021

Preprint

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The ever-increasing spectrum of microbial disease and emergence of life-threatening antibiotic-resistant pathogens necessitates more intensive search for microbial antagonistic agents from diverse symbionts and habitats. Based on these hypotheses the current study was initiated to investigate in vitro antibacterial and antifungal activities of both crude and pure compounds isolated from potential actinomycete isolates recovered from soda lakes of Ethiopia. Two soda lakes, Arenguade/Hora Hadho/ and Chitu were included in this study. A total of fifteen 12 water samples were collected and actinomycete cultures were isolated by serial dilution plating technique on various media. Morphological and biochemical characterization of selected isolates was done as described in the International Streptomyces Project Guidelines. Minimum inhibitory concentrations of the crude extract was determined for fungus using the protocol described in National Committee for Clinical Laboratory Standards and for the bacteria disc diffusion assay method as described in the Clinical and Laboratory Standards Institution. Ethanol extracts obtained from Solid State Fermentation a total of 36 isolates of actinomycetes were subjected to primary screening and tested for activity against the bacterial and fungal reference strain. Accordingly, the extracts from 10 isolates that were most active in the primary screen were 6 (16.66%) from Lake Chitu that were inhibited the growth of Gram-positive bacteria and fungal strain; 4 (11.11%) from Lake Arenguade that were inhibited Gram-negative bacteria and fungal. From evaluated the antibacterial and antifungal potential of two soda lakes water-derived actinomycetes; the best ones in terms of activity spectra were actinomycetes species isolated from Lake Chitu. Antibacterial and antifungal metabolite production was detected from isolates of Lake Chitu with maximum zone of inhibition within 5–6 days of incubation against Candida albicans ATCC 62376, Aspergillus niger, C. neoformans, F. oxysporum f. sp. melonis (CECT 20474), and Shigella boydii (clinical). All test microorganisms, except Staphylococcus aureus ATCC25923, E. coli ATCC 25922, and S. aureus (clinical isolate) were sensitive to the culture filtrate starting from 8–12 days of incubation. In terms of the effectiveness of the antibacterial activity, the antibacterial metabolite produced from different carbon sources were more effective on S. aureus (clinical isolate), A. niger, C. neoformans, S. aureus ATCC25923, Fusarium oxysporum f. sp. melonis (CECT 20474), Shigella boydii (clinical isolate) respectively. However, the most resistant test isolates with least activity was detected against Candida albicans ATCC 62376 and E. coli ATCC 25922.

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Section: Discussionsupporting

confidence: 76%

In vitro bioassay of antibacterial and antifungal activity studies of actinomycetes from soda lakes of Ethiopia

Chali

Belay

Bacha

2021

Preprint

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“…Bacillus sp. yang diisolasi dari sampel sedimen mangrove berpotensi untuk menghambat patogen Aermonas sp., Vibrio sp., Malassezia globosa dan Malassezia furfur (Arumugam et al, 2017;Sangkanu et al (2017). Oleh karena itu, penelitian ini bertujuan untuk memperoleh dan menentukan jenis bakteri dari sedimen mangrove yang memiliki aktivitas antijamur terhadap C. albicans dan M. furfur melalui uji biokimia.…”

Section: Pendahuluanunclassified

Aktivitas Antijamur dari Bakteri Sedimen Mangrove Terhadap Candida albicans dan Malassezia furfur

2022

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Candida albicans and Malassezia furfur are fungal pathogens that can cause skin infections. This disease can be easily transmitted from one individual to another. The use of antibiotic drugs can cause pathogenic fungi to become resistant. Therefore, there is a need for the development of new antifungal substances, one of which comes from mangrove sediment bacteria. This research was conducted with the aim of obtaining bacterial isolats from mangrove sediments that have the ability to fight the fungal pathogens Candida albicans and Malassezia furfur. The method in this research is descriptive exploratory laboratory which includes isolation sampling and isolat purification, morphological characterization, screening, antifungal activity test and biochemical test. The results of this study were obtained 3 bacterial isolats that have antifungal activity against the fungal pathogens Candida albicans and Malassezia furfur. The results of the identification of biochemical tests showed that the isolat S.ISP2 was identified as the genus Pseudomonas; isolat S.ISP4 identified as Genus Moraxella; and isolat KJ.MA1 identified as Genus Vibrio Candida albicans dan Malassezia furfur merupakan patogen jamur yang dapat menyebabkan penyakit infeksi kulit. Penyakit ini dapat dengan mudah ditularkan dari satu individu ke individu lainnya. Penggunaan obat antibiotik dapat mengakibatkan jamur patogen menjadi resisten. Oleh karena itum eprlunya ada pengembangan zat antijamur baru yang salah satunya berasal dari bakteri sedimen mangrove. Penelitian ini dilakukan dengan tujuan untuk mendapatkan bakteri isolat dari sedimen mangrove yang memiliki kemampuan untuk melawan jamur patogen Candida albicans dan Malassezia furfur. Metode pada penelitian ini adalah deskriptif eksploratif laboratory yang meliputi sampling isolasi dan purifikasi isolat, karakterisasi morfologi, skrining, uji aktivitas antijamur dan uji biokimia. Hasil dari penelitian ini adalah didapatkan 3 bakteri isolat yang memiliki aktivitas antijamur terhadap jamur patogen Candida albicans dan Malassezia furfur. Hasil identifikasi uji biokimia menunjukkan bahwa isolat S.ISP2 teridentifikasi sebagai Genus Pseudomonas; isolat S.ISP4 teridentifikasi sebagai Genus Moraxella; dan isolat KJ.MA1 teridentifikasi sebagai Genus Vibrio.

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“…Đây là một phát hiện vô cùng quan trọng trong thời điểm hiện tại vì hệ vi khuẩn trong một biofilm của những vi khuẩn gây bệnh, đặc biệt với S. aureus là chủng gây bệnh nhiễm trùng máu, vì thực sự chúng có nhiều biến đổi về gen và đa số là kháng thuốc kháng sinh hiện hành [10]. Một nghiên cứu khác tìm kiếm vi sinh vật từ rừng ngập mặn cho mục đích tìm kiếm các chủng có khả năng sinh kháng sinh cho thấy có đến 26,38% của 144 chủng có hoạt tính hai chủng đang được nghiên cứu tiếp theo để tìm kiếm các enzyme phân hủy cellulose, sinh enzyme protease, amylase nhằm tạo ra sản phẩm sinh học ứng dụng trong nông nghiệp và thủy sản [11]. Chất chiết từ nấm Trichoderma có nguồn gốc từ rừng ngập mặn Pichavaram (Ấn Độ) chống lại vi khuẩn gây bệnh cho người và cho cá [12].…”

Section: Mở đầUunclassified

Sàng Lọc Vi Khuẩn Có Khả Năng Kháng Khuẩn Từ Rừng Ngập Mặn Ninh Ích Ninh Hòa Khánh Hòa

Mien

Khoa²

2020

VJMST

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In the context of global climate changes, mangroves are not only considered as an important ecological region but also as a source of exploitation of specific biological objects for researches and applications. In this study, mangrove-derived bacteria were isolated and screened for antimicrobial producers. Also, the strains with potential antibacterial activity were identified by 16S rRNA gene analysis. Results showed that 43% of 21 strains possessed antibacterial activity. The strain R10 showed inhibition against all four indicator bacteria and was identified as Bacillus pumilus with the highest sequence similarity of 100% to Bacillus pumilus NCTC10337 (GenBank accession number LT906438.1). The strain R7 inhibited Serratia marcescens causing severe “white spot” disease in reef building coral Acropora palmata and was closely related to Bacillus toyonesis BCT 7112 (GenBank accession number NR121761.1) with 99% sequence similarity. The strain R3 was the strongest strain against Bacillus subtilis compared with other strains which revealed the same antimicrobial activity pattern. The strain R3 was affiliated with Bacillus marisflavi TF-11 (GenBank accession number NR025240.1) with 98% sequence similarity and may be considered to represent new species. Moreover, the strain R9 inhibited only Gram negative tested strain and this strain was considered as a potential candidate for further search of antibiotics with spectrum of Gram negative bacteria.

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Screening of novel actinobacteria and characterization of the potential isolates from mangrove sediment of south coastal India

Cited by 25 publications

References 29 publications

In vitro bioassay of antibacterial and antifungal activity studies of actinomycetes from soda lakes of Ethiopia

In vitro bioassay of antibacterial and antifungal activity studies of actinomycetes from soda lakes of Ethiopia

Aktivitas Antijamur dari Bakteri Sedimen Mangrove Terhadap Candida albicans dan Malassezia furfur

Sàng Lọc Vi Khuẩn Có Khả Năng Kháng Khuẩn Từ Rừng Ngập Mặn Ninh Ích Ninh Hòa Khánh Hòa

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