Screening of nonpolyenic antifungal metabolites produced by clinical isolates of actinomycetes

Lemriss, Sanaâ; Laurent, François; Couble, A.; Casoli, E; Lancelin, Jean‐Marc; Saintpierre-Bonaccio, D.; Rifai, Saida; Fassouane, A.; Boiron, P.

doi:10.1139/w03-088

Cited by 38 publications

(28 citation statements)

References 14 publications

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“…In this study, the total number of isolated actinomycetes (117) was screened on agar medium and antibacterial activity was observed in 15 (12.82 %) of the isolates and appeared promising ( Table 1). In previous studies, it was shown that the isolation rate of actinomycetes with antimicrobial activity is higher than 40% [15] and in others less than 10% [16]. These results confirm that the actinomycetes are able to produce a wide variety of antibiotics with antibacterial activity.…”

Section: Resultssupporting

confidence: 69%

Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria

Kumar¹,

Singh²,

Mishra³

et al. 2010

Int J of Micr Res

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Abstract-A total of 117 actinomycetes strains were isolated from the wasteland alkaline and garden soil samples of the Ghaziabad and screened for their anti-bacterial activity. They were evaluated for their inhibitory activities on four test microorganisms. Fifteen actinomycetes isolate which exhibited antimicrobial activity against at least two of the test organisms and were characterized by conventional methods. The cultural characteristics of isolates were also studies in different culture media. The results indicated that six isolates were highly active against Staphylococcus aureus strains. Seven isolates were highly active with an inhibition zone more than 20 mm in diameter. Most of the isolates inhibited growth of the Gram negative bacteria tested. All the antibiotic producing actinomycetes were isolated at different temperatures from non agricultural wasteland alkaline soil and compost rich garden soil. Fifteen isolates showed activity against bacteria in which most of them from wasteland alkaline soil where the less interference by human for agriculture or other purpose. These microorganisms may have capability to produce some of the most important medicines ever developed.

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Section: Resultssupporting

confidence: 69%

Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria

Kumar¹,

Singh²,

Mishra³

et al. 2010

Int J of Micr Res

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“…Indeed, utilization of some sources of carbon and nitrogen (starch, chitin, glycerol, casein, arginine and asparagine) makes the culture medium less favorable to the development of other bacteria (Cavalla and Eberlin, 1994). Five different colonies ( Figure 1) were isolated from soil and one from deteriorate wood; this is consistent with literature showing that soil is the main reservoir of actinomycetes (Lemriss et al, 2003).…”

Section: Isolation Of Actinomycetessupporting

confidence: 89%

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Jihani¹,

Brodolin²,

Iraqui³

et al. 2012

Afr. J. Microbiol. Res.

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“…Inhibition diameters were determined after 24 h for bacterial strains and 48 h for C. albicans. Plates were examined for evidence of antimicrobial activities, represented by a zone of inhibition of microbial growth around the cylinders (Lemriss et al, 2003;Mellouli et al, 2003;Kitouni et al, 2005).…”

Section: Agar Cylinders Methodsmentioning

confidence: 99%

Antimicrobial activities of culturable microorganisms (actinomycetes and fungi) isolated from Chaabe Cave, Algeria

Belyagoubi¹,

Belyagoubi-Benhammou²,

Jurado³

et al. 2018

IJS

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Abstract:The discovery of new antibiotics and other bioactive microbial metabolites is a priority given the frequency of the emerging multi-drug resistant pathogenic microorganisms. Thus, scientists are searching for new antibiotics in microorganisms isolated from extreme habitats such as caves. In the present study, we aimed to perform the first analysis on culturable microorganisms in the Chaabe Cave (Algeria), and to test the antimicrobial activities of the isolates (Streptomyces spp. and Penicillium spp.). The potential for antimicrobial activity of 47 strains of actinomycetes and 23 strains of fungi were tested on Candida albicans, Staphylococcus aureus, Micrococcus luteus, Listeria monocytogenes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The identification of the most active actinomycetes strains by 16S rRNA gene sequence analysis revealed that these isolates were exclusively affiliated to the genus Streptomyces. On the other hand, the fungi were determined by phylogenetic analysis based on the ITS region or on the β tubulin gene and were affiliated to the genera Readeriella, Cladosporium, Aspergillus, Penicillium, Beauveria, Alternaria, Rhizopus, and Rhizomucor. The strains showed strong inhibitory activity against pathogenic microorganisms and the diameters of the inhibition zones vary between 7.5 and 34 mm for Streptomyces strains, and between 6.5 and 19.50 mm for Penicillium strains. The data indicated that the majority (72.86%) of the 70 isolates were active against at least one of the tested microorganisms. The production of nonpolyenic antifungal substances by active Streptomyces isolates was investigated using several criteria (antibacterial activity, ergosterol inhibition, and UV-visible spectra) of active extracts. The results were promising and showed that the metabolites produced by the actinomycete strains do not have a UVvisible spectrum characteristic of a polyenic structure. The Chaabe Cave possesses a diversity of microorganisms that could lead to new antibiotics necessary in the fight against drug-resistant pathogens and warrant further study.

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Screening of nonpolyenic antifungal metabolites produced by clinical isolates of actinomycetes

Cited by 38 publications

References 14 publications

Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria

Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Antimicrobial activities of culturable microorganisms (actinomycetes and fungi) isolated from Chaabe Cave, Algeria

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