Screening of new bacterial isolates with antifungal activity and application of selected Bacillus sp. cultures for biocontrol of Fusarium graminearum under field conditions

Stumbriene, Karina; Gudiukaitė, Renata; Semaškienė, Roma; Svegzda, Povilas; Jonavičienė, Akvilė; Supronienė, Skaidrė

doi:10.1016/j.cropro.2018.07.006

Cited by 21 publications

(15 citation statements)

References 43 publications

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“…In addition to some bacteria (Stumbriene et al ; Volpiano et al ), mainly fungi of the genus Trichoderma (John et al ; Kotasthane et al ; Morales‐Rodriguez et al ; Alamri et al ) have been used for the biocontrol of soil‐borne diseases of Amorphophallus , Pinus radiata and lettuce caused by S. rolfsii and Fusarium spp. This study was to investigate the biocontrol effects of a new species P. griseofulvum and its mechanisms against two pathogens ( S. rolfsii and F. oxysporum ) of A. carmichaelii .…”

Section: Discussionmentioning

confidence: 99%

“…rolfsii and Fusarium spp. are mainly fungi of the genus Trichoderma (John et al ; Kotasthane et al ; Morales‐Rodriguez et al ; Alamri et al ), in addition to some bacteria (Stumbriene et al ; Volpiano et al ). These biocontrol micro‐organisms may control plant fungal pathogens by hyperparasitism, antibiosis, cell‐wall‐degrading enzymes and induction of plant resistance (Harman et al ; Lugtenberg and Kamilova ).…”

Section: Introductionmentioning

confidence: 99%

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Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Qiao

Wei

et al. 2019

J Appl Microbiol

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Aims: The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. Methods and Results: The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotiaformation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1Á9 9 10 10 conidia per gram of substrate) reduced soil-borne disease severity by 15Á0%. The shoot and root growth of A. carmichaelii plants was promoted by 61Á6 and 83Á1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. Significance and Impact of the Study: Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Qiao

Wei

et al. 2019

J Appl Microbiol

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“…An aliquot of 1 mL was taken from each of the 40 cultures and filtered through 0.22 µm filters (Merck Millipore, United States). Two 200 µL droplets of each filtrate were placed on a plate 2 cm away from a pathogen plug following Stumbriene et al (2018). Antagonism was assessed by examining the inhibition of pathogen growth as it approached the microfloral culture filtrate and the presence of inhibition zones.…”

Section: Culture and Screening Of Functional Microfloramentioning

confidence: 99%

“…However, many disadvantages have been found during the use of those traditional approaches, such as the emergence of fungicide-resistant populations, disruption of local ecosystems, large labor requirements, and threats to human health. As such, it may be better to use several control methods simultaneously (Stumbriene et al, 2018). Therefore, it is urgent to develop effective and environmentally friendly methods to control N. tabacum diseases.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, many microbial ecological agents have been isolated from plant tissues and soil samples to control plant diseases. Bacillus spp., isolated from wheat roots and soil samples, could effectively reduce the growth of Fusarium graminearum strains causing Fusarium head blight (Stumbriene et al, 2018); Bacillus subtilis Tpb55, isolated from N. tabacum leaves, could effectively control the hyphal growth of P. nicotianae and had an obvious suppressive effect on black shank (Han et al, 2016); Flavobacterium sp. TRM1, isolated from rhizosphere soil, could suppress Ralstonia solanacearum disease development (Kwak et al, 2018); Pseudomonas aeruginaosa NXHG29, isolated from soil (Li et al, 2012), could decrease the incidence of N. tabacum bacterial wilt and black shank (Ma et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Cultured Root and Soil Microbial Communities on the Disease of Nicotiana tabacum Caused by Phytophthora nicotianae

et al. 2020

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Background: Black shank, caused by the oomycete pathogen Phytophthora nicotianae, is responsible for huge economic losses worldwide. Research has focused on biocontrol to prevent disease and to minimize the use of synthetic fungicides. Methods:We explored and compared the efficacy of suppressive microflora cultured from soil and roots on the growth of P. nicotianae for controlling the incidence of black shank.Results: We found that 31 microfloral communities, enriched from 40 root samples but only 18 microfloral communities from soil samples, were antagonistic to P. nicotianae. In the field experiment, the root functional microflora (RFM) showed a greater suppressiveness of black shank than the soil functional microflora (SFM), while both RFM and SFM altered diversity, composition, structure, and interaction of soil bacterial communities during plant growth. Although the inoculation of RFM onto roots significantly (p < 0.05) decreased microbial diversity, molecular ecological network analysis indicated more possible interactions among soil microbes, while an opposite trend was observed with SFM inoculation. Linear regression analysis revealed that diversity indices were negatively correlated with suppression on the black shank, suggesting that specific taxa (e.g., OTU_322 and OTU_6478) could colonize and be active during plant growth at the expense of microbial diversity. In addition, 18 functional strains, isolated and screened from 3 RMF (12 strains) and 3 SMF (6 strains), were identified as bacterial genera Acinetobacter (12), Enterobacter (1), Bacillus (1), Stenotrophomonas (2), and Citrobacter (2). Spearman's ranked correlation tests revealed that relative abundances of some OTUs affiliated with genera Acinetobacter, Enterobacter, and Bacillus were significantly (p < 0.05) and positively correlated with the level of disease suppression. Conclusion:Microfloral communities or key functional species isolated from plant roots might be more effective in controlling black shank than those from soil, and they may be developed for disease control.

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Phytosanitary Monitoring of Grain Crops and Soybeans in the Khabarovsk Territory

Tishkova

Imtosimi

2021

Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East (AFE-2021)

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Screening of new bacterial isolates with antifungal activity and application of selected Bacillus sp. cultures for biocontrol of Fusarium graminearum under field conditions

Cited by 21 publications

References 43 publications

Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Effects of Cultured Root and Soil Microbial Communities on the Disease of Nicotiana tabacum Caused by Phytophthora nicotianae

Phytosanitary Monitoring of Grain Crops and Soybeans in the Khabarovsk Territory

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