Screening of microorganisms for bioconversion of (−)β-pinene and R-(+)-limonene to α-terpineol

On the current role of hydratases in biocatalysis

“…Due to its floral odour, ( R )-(+)-α-terpineol is an essential raw material for the food and cosmetics industries. It is produced chemically at low costs by acid catalysed hydration and partial dehydration of pinene or crude turpentine oil (Rottava et al 2010). However, in view of the increasing efforts industry is devoting to the production of natural flavours and fragrances, biotransformations routes towards enantiopure ( R )-(+)-α-terpineol are highly desirable (Adams et al 2003; Ran et al 2008).…”

“…In general, α-terpineol production from limonene has been reported in a plethora of different bacteria and yeasts, including Bacillus stearothermophilus , Pseudomonas gladioli , Sphingobium sp., Aspergillus sp., Fusarium oxysporum and Penicillium digitatum (Duetz et al 2003; Maróstica and Pastore 2006). However, considering that biotransformations of limonene were performed almost exclusively in whole cells with the co-production of many other limonene derivatives (Tan and Day 1998; Adams et al 2003; Duetz et al 2003; Kaspera et al 2005; Rottava et al 2010), specific enzymes for limonene hydration are almost never described. For instance, a hydration reaction was initially discussed for the highly selective production of (4 R )-(+)-α-terpineol from ( R )-(+)-limonene in P. digitatum , but later revised to a two-step reaction comprising an epoxidation and oxidative cleavage of the 8,9-double bond (Abraham et al 1986; Tan et al 1998; Pescheck et al 2009; Badee et al 2011).…”

Fractionation of lemon essential oil by solvent extraction: Phase equilibrium for model systems at T=298.2K

“…Many techniques, such as supercritical fluid extraction, membrane processing, supercritical adsorption/ desorption, vacuum and steam distillations, enzymatic conversion, selective inclusion and solvent (or liquid-liquid) extraction, have been used to deterpenate essential oils [6][7][8][9][10][11][12].…”

Optimisation of α‐terpineol production by limonene biotransformation using Penicillium digitatumDSM 62840

“…To determine the effect of temperature on biotransformation, cultures were incubated at different transformational temperatures (6,8,16,24,28,32,36, and 40 ∘ C) after 48 h of pre-culture at 24 ∘ C and 150 rpm. A total of 50 μL of pure (R)-(+)-limonene substrate was added.…”

“…Biotransformation can be defined as chemical reactions catalysed by enzymes of microorganisms, using purified enzymes, grown cultures, resting cells, or even immobilised cells in shake flasks, bioreactors, or plates . Biotransformation is known to be a useful alternative to chemical synthesis because of the marked regioselectivity and enantioselectivity.…”