Screening of lipases for the synthesis of xylitol monoesters by chemoenzymatic esterification and the potential of microwave and ultrasound irradiations to enhance the reaction rate

Rufino, Alessandra R.; Biaggio, Francisco C.; Santos, Júlio César dos; Castro, Heizir F. de

doi:10.1016/j.ijbiomac.2010.04.009

Cited by 29 publications

(14 citation statements)

References 23 publications

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“…In another study, the enzymatic synthesis of isoamyl myristate by esterification reaction in a solvent-free system catalyzed by Novozym 435 was also improved by microwave irradiation [54]. The productivity of xylitol monoesters synthesis catalyzed by immobilized lipase from Penicillium camemberti was higher under nonconventional heating than conventional one, 5.3 mmol/h and 2.2 mmol/h, respectively [29]. …”

Section: Resultsmentioning

confidence: 99%

“…Moreover, experiments were also carried out to determine the feasibility of using nonconventional heating systems such as microwave irradiation. Microwave, a nonionizing radiation has been explored in recent years as an alternative heating system in many areas, including the enzymatic biodiesel synthesis [27–29]. Microwave heating involves directed absorption of energy by functional groups that bear ionic conductivity or a dipole rotational effect, and this energy is then released into the surrounding solution [28].…”

Section: Introductionmentioning

confidence: 99%

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Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Souza

Mendes

Castro

2016

BioMed Research International

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The present study deals with the enzymatic synthesis of biodiesel by transesterification of Jatropha oil (Jatropha curcas L.) with ethanol in a solvent-free system. Seven commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-polyvinyl alcohol composite (epoxy-SiO2-PVA) were tested as biocatalysts. Among them, immobilized lipases from Pseudomonas fluorescens (lipase AK) and Burkholderia cepacia (lipase PS) were the most active biocatalysts in biodiesel synthesis, reaching ethyl ester yields (FAEE) of 91.1 and 98.3% at 72 h of reaction, respectively. The latter biocatalyst exhibited similar performance compared to Novozym® 435. Purified biodiesel was characterized by different techniques. Transesterification reaction carried out under microwave irradiation exhibited higher yield and productivity than conventional heating. The operational stability of immobilized lipase PS was determined in repeated batch runs under conventional and microwave heating systems, revealing half-life times of 430.4 h and 23.5 h, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Souza

Mendes

Castro

2016

BioMed Research International

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“…However, the catalytic activity of the four commercial lipase preparations displayed similar performance on butyl butyrate synthesis. According to data reported in the literature, lipases from T. lanuginosus and P. fluorescens are very efficient in emulsified triglycerides hydrolysis, but the enzymes could be inactivated in organic medium [8,11,16,25]. These lipase preparations are "typical lipases" with a large lid domain that covers their active sites [25].…”

Section: Butyl Butyrate Synthesismentioning

confidence: 99%

“…In organic medium, lipases also catalyze esterifications, transesterifications and interesterifications [1][2][3][4]. These enzymes are applied in several industrial processes including synthesis of biopolymers, production of pharmaceuticals, agrochemicals, cosmetics, flavors, treatment of waste rich in lipids and biodiesel synthesis by transesterification of triglycerides with short-chain alcohols [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immobilized lipases on poly-hydroxybutyrate beads to catalyze biodiesel synthesis

Mendes

Oliveira

Velez

et al. 2012

International Journal of Biological Macromolecules

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Five microbial lipase preparations from several sources were immobilized by hydrophobic adsorption on small or large poly-hydroxybutyrate (PHB) beads and the effect of the support particle size on the biocatalyst activity was assessed in the hydrolysis of olive oil, esterification of butyric acid with butanol and transesterification of babassu oil (Orbignya sp.) with ethanol. The catalytic activity of the immobilized lipases in both olive oil hydrolysis and biodiesel synthesis was influenced by the particle size of PHB and lipase source. In the esterification reaction such influence was not observed. Geobacillus thermocatenulatus lipase (BTL2) was considered to be inadequate to catalyze biodiesel synthesis, but displayed high esterification activity. Butyl butyrate synthesis catalyzed by BTL2 immobilized on small PHB beads gave the highest yield (≈90 mmol L(-1)). In biodiesel synthesis, the catalytic activity of the immobilized lipases was significantly increased in comparison to the free lipases. Full conversion of babassu oil into ethyl esters was achieved at 72 h in the presence of Pseudozyma antarctica type B (CALB), Thermomyces lanuginosus lipase (Lipex(®) 100 L) immobilized on either small or large PHB beads and Pseudomonas fluorescens (PFL) immobilized on large PHB beads. The latter preparation presented the highest productivity (40.9 mg of ethyl esters mg(-1) immobilized protein h(-1)).

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“…Tabela 19: Comparação dos parâmetros globais de reação de etanólise do óleo de pinhão manso (rendimento e produtividade), para os experimentos realizados sob aquecimento convencional e micro-ondas (6h), utilizando lipase PS imobilizada em SiO 2 -PVA. (DA RÓS, 2009;RUFINO et al, 2010).…”

Section: Estabilidade Operacional Da Lipase Ps Imobilizada Em Sio 2 -Pvaunclassified

Síntese enzimática do biodiesel de Jatropha curcas pela rota etílica

Souza¹

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The expected increase in the biodiesel demand worldwide has brought a constant evolution in its production system in order to make it more efficient and environmentally favorable. The objective of present work was to verify the potential of Jatropha oil as raw material to produce biodiesel by enzymatic route using ethanol as acilant agent. To attain the proposed objective, the experimental activities were starting by treating the oil to attain suitable properties to be used in the transesterification reaction, including the degumming, neutralization and drying steps. The treated oil, after physico-chemical characterization was used to carry out a screening test to select the most suitable biocatalyst by means of testing different preparations of lipases (EC 3.1.1.3) in free form as well as immobilized in SiO 2-PVA, to mediate the biodiesel synthesis in solvent free system. The assays indicated that the immobilized lipases were more efficient than free ones and allowed selecting the immobilized derivatives from Burkholderia cepacia and Pseudomonas fluorescens as the most suitable preparations to catalyze biodiesel synthesis from Jatropha oil, attaining yields of 93.18% and 85.67%, respectively. In the second step, the selected immobilized derivatives were used to catalyze the reaction of interest maintaining the previous set conditions (temperature 45 o C, 1:9 molar ratio oil/ethanol and 500 units of lipolytic activity per gram of oil) using a glass reactor coupled with condenser to avoid ethanol loss. The reaction was monitored by determining the formed ethyl esters by gas chromatography and viscosity in samples taken from the reactor during the reaction. The transesterified product (biodiesel) was purified and submitted to further analyses for physico-chemical properties, including rheological study, FTIR, TG and 1 H NMR. The obtained results confirmed that the lipase from Burkholderia cepacia was the most efficient biocatalyst to mediate the biodiesel synthesis from Jatropha oil, attaining transesterification yields higher than 97% (72h. The product biodiesel was thermo stable up to 128 o C and no residual glycerol or water contaminations were detected, assuring the efficiency of the down stream process. Additional experiments were performed under microwave irradiation and the results suggested that the microwave heating constitutes a potential procedure to enhance the reaction rate by reducing the global reaction time. The operational stability of the immobilized lipase was determined in repeated batch runs under conventional and microwave heating systems, revealing biocatalyst half-life time of 110 and 26.5 h, respectively. Therefore, the real contribution of the microwave irradiations to enhance the reaction should be revalued by taking into account the lost of the biocatalyst activity.

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Screening of lipases for the synthesis of xylitol monoesters by chemoenzymatic esterification and the potential of microwave and ultrasound irradiations to enhance the reaction rate

Cited by 29 publications

References 23 publications

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate

Evaluation of immobilized lipases on poly-hydroxybutyrate beads to catalyze biodiesel synthesis

Síntese enzimática do biodiesel de Jatropha curcas pela rota etílica

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