Screening of Larvicidal and Pupicidal Activity Of Biogenic Silver Nanoparticles (AgNPs) of Bidens biternata(L) against the Dengue & Chikungunya Vector, Aedes Aegypti

Abstract

Mosquitoes are one of the most medically noteworthy groups of vectors, having an capacity to spread parasites and pathogens that can have devastating impacts on humans. The development of reliable and ecofriendly method for the synthesis of metallic nanoparticles is an key step in the field of application of nanotechnology [7]. In this study, we address the biosynthesis of silver nanoparticles (AgNPs) using leaf extract of Bidens biternata (L), and evaluate its lethal concentration (LC50) values against fourth instar larvae and pupae of the mosquito vectors, Aedes aegypti. The nanoparticles were characterized by UV-Vis spectrum, XRD Analysis, scanning electron microscopy, EDAX, and Fourier-transformed infrared spectroscopy analysis. Larvae and pupae were exposed to different concentrations of aqueous extracts of biogenic AgNPs for 24. The larval mortality was increased from 30-100% with increasing concentration from 1-10 ppm. The total mortality of IV Instars larvae of Ae. aegypti was increased from 20-100% with increasing concentration o aqueous leaf extract of Bidens biternata (L) from 10-100 ppm. The LC50 of the AgNPs synthesized from the leaf extract of Bidens biternata (L) against Ae. aegypti was 5.261 ppm.