Screening of Lactobacilli from Fish Intestines to Select a Probiotic for Warm Freshwater Fish

Bucio, Adolfo; Hartemink, R.; Schrama, J.W.; Rombouts, F.M.

doi:10.12938/bifidus.23.21

Cited by 9 publications

(8 citation statements)

References 32 publications

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“…The strain Lact. plantarum 44a used in this study had been previously isolated from the intestinal content of European wild eel ( Anguilla anguilla ) (Bucio et al. 2004, 2006).…”

Section: Methodsmentioning

confidence: 99%

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Kinetics ofLactobacillus plantarum44a in the faeces of tilapia (Oreochromis niloticus) after its intake in feed

Galindo

Hartemink

Schrama

et al. 2009

Journal of Applied Microbiology

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Aims: To study the kinetic passage of Lactobacillus plantarum 44a from feed to faeces of tilapia in order to calculate the number of Lactobacillus excreted. Methods and Results: In a single‐dose experiment, duplicate lots of 26 fish devoid of intestinal lactobacilli were fed with diets containing c. 4·5 × 1011, 6·3 × 108, 6·0 × 105 and 0 CFU of Lact. plantarum 44a per single feed ration. In the multiple‐dose experiment, duplicate lots of 30 fish each were supplied with a diet containing 1 × l011 CFU of Lact. plantarum 44a as follows: 14 times in 14 days, five times in 14 days, once in 14 days and zero times in 14 days. Faeces were periodically collected and analysed for their lactobacilli content by using a selective media. The kinetics of Lactobacillus in the faeces was described as a pulse signal defined by three parameters: θ, Ao and the time. θ, was identified as the time to reach the peak (x axis) and Ao was a constant. Ao divided by e, was identified as the height of the peak (y axis). The area below the curve Aoθ allowed the calculation of the total number of lactobacilli excreted. The ability of the mathematical model to describe the actual values was tested by a linear regression analysis. In most of the cases, the equations showed an intercept close to zero (P > 0·05) and angular coefficients near one. Conclusions: Lactobacillus plantarum 44a was excreted in short pulse signals described by a mathematical model which allowed calculating the area below the curve and consequently the survival rate. Significance and Impact of the Study: This study provides a quantitative method to study the kinetics of excretion of a probiotic bacterium in the faeces.

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“…The strain Lact. plantarum 44a used in this study had been previously isolated from the intestinal content of European wild eel ( Anguilla anguilla ) (Bucio et al. 2004, 2006).…”

Section: Methodsmentioning

confidence: 99%

“…This was done after in vitro screening for inhibitory activities against common pathogens like Aeromonas hydrophila and Edwardsiella tarda . There was a high survival rate in vitro in a gastrointestinal tract model, an absence of amine production (Bucio et al. 2004) and survival after spraying, drying and storage of feed (Bucio et al.…”

Section: Introductionmentioning

confidence: 99%

Kinetics ofLactobacillus plantarum44a in the faeces of tilapia (Oreochromis niloticus) after its intake in feed

Galindo

Hartemink

Schrama

et al. 2009

Journal of Applied Microbiology

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show abstract

“…Pengukuran zona bening dilakukan dengan menggunakan jangka sorong pada empat posisi diameter lingkaran dari setiap kertas cakram. Berdasarkan hasil perhitungan, diperoleh aktivitas antagonistik bakteri termasuk dalam kriteria sedang dengan rata-rata diameter zona hambat yang terbentuk sebesar 5,87 ± 0,43 mm (Bucio et al, 2004).…”

Section: Penentuan Aktivitas Antagonistikunclassified

“…Sedangkan pada perlakuan lainnya diduga jumlah Lactobacillus sp. kurang dari 10 2 (CFU/ gram) yang merupakan batas pengamatan pada penelitian ini Bucio et al (2004). menyatakan bahwa L. brevis strain 18 f ditemukan pada usus bagian atas.…”

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PEMBERIAN PROBIOTIK LACTOBACILLUS BREVIS DAN PREBIOTIK OLIGOSAKARIDA PADA BENIH PATIN SIAM (Pangasionodon hypophthalmus) YANG DIINFEKSI Aeromonas hydrophila

Eliyani

Widanarni

Wahjuningrum

2013

Jurnal Riset Akuakultur

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Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian probiotikLactobacillus brevis, prebiotik oligosakarida dan sinbiotiknya terhadap jumlah bakteri Lactobacillus sp. dan total bakteri dalam usus, total eritrosit, total leukosit, diferensial leukosit, aktivitas fagositik, sintasan, tingkat pertumbuhan, serta FCR benih ikan patin siam yang diinfeksi Aeromonas hydrophila. Hasil uji karakteristik menunjukkan bahwa jenis probiotik dan patogen adalah Lactobacillus sp. dan Aeromonas hydrophila. Pada uji in vivo digunakan lima perlakuan yang terdiri atas K(+), K(-), probiotik (pro), prebiotik (pre) serta sinbiotik (sin). Bakteri Lactobacillus sp. ditemukan di usus pada perlakuan probiotik dan sinbiotik dengan kisaran jumlah sekitar 101 sampai 106 (CFU/g). Total eritrosit, total leukosit, aktivitas fagositik berbeda nyata (P<0,05) dengan kontrol pada beberapa waktu pengamatan. Tingkat sintasan terendah diperoleh pada perlakuan K(+) sebesar 43,33±11,55; sedangkan empat perlakuan lainnya memperoleh nilai 100%. Tingkat pertumbuhan harian berbeda nyata antar perlakuan, nilai terbaik dicapai pada perlakuan pemberian sinbiotik sebesar 3,370±0,14. Nilai FCR perlakuan probiotik, prebiotik dan sinbiotik menunjukkan beda nyata dengan kontrol. Perlakuan sin, pre, pro memberikan nilai yang lebih baik pada total eritrosit, total leukosit, aktivitas fagositik, sintasan, pertumbuhan, dan FCR dibandingkan kontrol.

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“…We selected Lactobacillus plantarum 44a for our studies because it is a probiotic candidate for warm freshwater fish as it displays inhibitory activities against common fish and human pathogens, and it is tolerant to the intestinal environment, being acid tolerant and fish bile tolerant (Bucio et al, 2004).…”

Section: Full Papermentioning

confidence: 99%

Survival of Lactobacillus plantarum 44a after spraying and drying in feed and during exposure to gastrointestinal tract fluids in vitro

Bucio

Hartemink

Schrama

et al. 2005

J. Gen. Appl. Microbiol.

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Screening of Lactobacilli from Fish Intestines to Select a Probiotic for Warm Freshwater Fish

Cited by 9 publications

References 32 publications

Kinetics ofLactobacillus plantarum44a in the faeces of tilapia (Oreochromis niloticus) after its intake in feed

Kinetics ofLactobacillus plantarum44a in the faeces of tilapia (Oreochromis niloticus) after its intake in feed

PEMBERIAN PROBIOTIK LACTOBACILLUS BREVIS DAN PREBIOTIK OLIGOSAKARIDA PADA BENIH PATIN SIAM (Pangasionodon hypophthalmus) YANG DIINFEKSI Aeromonas hydrophila

Survival of Lactobacillus plantarum 44a after spraying and drying in feed and during exposure to gastrointestinal tract fluids in vitro

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