Screening of Lactic Acid Bacteria from the Gut of Chrysichthys nigrodigitatus for Use as Probiotics in Aquaculture Production

Olalusi, C. I. Ayo; Oresegun, A.; Bernard, Edah

doi:10.3923/jfas.2014.478.482

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…While these approaches only aimed to reveal the ''variety'' constituted in the microbiome, some early studies also correlated the taxonomic abundance of fish gut flora with specific host functions [10,11]. Over the years, the culture based assessments have led to identification of several probiotic and pathogenic bacterial strains and still continue being employed in studies of fish gut microbiome [12][13][14]. In recent years, however, the cultureindependent methods have replaced these traditional techniques as the direct mining of community DNA hold promise to unveil the low abundance and rare taxa [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Fish Gut Microbiome: Current Approaches and Future Perspectives

et al. 2018

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In recent years, investigations of microbial flora associated with fish gut have deepened our knowledge of the complex interactions occurring between microbes and host fish. The gut microbiome not only reinforces the digestive and immune systems in fish but is itself shaped by several host-associated factors. Unfortunately, in the past, majority of studies have focused upon the structure of fish gut microbiome providing little knowledge of effects of these factors distinctively and the immense functional potential of the gut microbiome. In this review, we have highlighted the recently gained insights into the diversity and functions of the fish gut microbiome. We have also delved on the current approaches that are being employed to study the fish gut microbiome with an aim to collate all the knowledge gained and make accurate conclusions for their application based perspectives. The literature reviewed indicated that the future research should shift towards functional microbiomics to improve the maximum sustainable yield in aquaculture.

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Section: Introductionmentioning

confidence: 99%

Fish Gut Microbiome: Current Approaches and Future Perspectives

et al. 2018

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“…Two individuals were selected according to their weight and height. The Zebrafish skin was washed with 70% ethanol and the bacterial strains were isolated from the Zebrafish gut by dissection method for analysis of zinc-resistant bacteria, this was assessed based on the protocol of C.I Ayo-Olalusi et al (2014) [15], [16].…”

Section: Sample Collection and Isolation Of Bacterial Strainsmentioning

confidence: 99%

“…The work exhibits newness to the study of novel bacterial species present in the zebrafish gut microbiome that shows resistance to heavy metals like zinc and correlated antibiotics resistance [15], [16]. Identification of new isolates is becoming increasingly important because they contain novel genes and metabolites that help in bioremediation.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Analysis of the Gut Microbiome of Zebrafish for Bioremediation Approach of Zinc-Contaminated Aquatic Environments

2023

JPTCP

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The discovery of bacterial organisms that aid in heavy metal bioremediation opens up new possibilities for removing radioactive compounds and heavy metals from polluted water sources. Accordingly, this study aims to the identification of Zinc resistant bacteria from the gut microbiome of zebrafish and to evaluate its bioremediation capability. The isolates, namely FG01 (Enterobacter cloacae), FG02 (Citrobacter freundii), and FG03 (Aeromonas hydrophila) identified based on 16S rRNA gene sequencing resistant to Zinc and antibiotics like Ampicillin and Amoxicillin and also selected for bioaccumulation studies. The MTC values of the three zinc-resistant bacteria from the Zebrafish gut were evaluated, and the results revealed that the growth of the isolate FG02 was better than the others, while the growth of FGO3 and FG01 decreased at the concentrations of 10 ppm and 15 ppm. By docking studies, the Zinc binding proteins (ZBPs) were discovered and beta-lactamase showed the best binding affinity compared to the other protein. In the process of treating wastewater, zinc-resistant bacteria from the gut microbiome of zebrafish are normally present and can demonstrate their capacity to adsorb heavy metals. The ZBP can be used later as a novel absorbent for heavy metal removal technology.

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Effect of Dietary Supplementation of Bacillus subtilis and Terribacillus saccharophillus on Innate Immune Responses of a Tropical Freshwater Fish, Labeo rohita

Kalarani¹,

Sumathi²

2016

J Clin Cell Immunol

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Objective: Indiscriminate use of antibiotics to control fish diseases has led to the development and propagation of antibiotic resistant bacteria in aquatic ecosystems. Amongst the various alternatives proposed, enhancing immunity in fish using probiotics is the most widely accepted approach. Several earlier studies indicated the possibility of more potent effects of bacteria on fish when administered in live condition than as inactivated cells. But very few studies have explored the effect of gut bacteria on innate immune responses of fishes. Hence an attempt is made to study immunostimulatory effects of dietary supplementation of Bacillus subtilis and Terribacillus saccharophillus isolated from the gut of an edible Indian freshwater fish, Labeo rohita, in the same species.Methods: Long term administration (for 30 and 60 days) of B. subtilis or T. saccharophillus isolated from the gut wall of L. rohita were tested in the same species for their ability to improve innate immunity through measurement of the phagocytic activity, respiratory burst, myeloperoxidase activity, serum IgM levels, serum lectins, haemagglutination and haemolytic activity.Results: Dietary administration of 107 cfu/g of B.subtilis/T. saccharophillus for 30 (B 1 ) or 60 (B 2 ) days resulted in a significant increase in immune and humoral responses of L. rohita. Serum phagocytic activity, respiratory burst activity, myeloperoxidase activity, serum lectins, haemagglutination and haemolytic activity increased upon administration of B.subtilis / T. saccharophillus, except serum IgM levels which increased only in B 1 . A positive correlation was observed between the duration of supplementation of diets and the responses in both the cases. Conclusion:The results suggest that T. saccharophillus can also be used as a potent probiont similar to B.subtilis and both will have significant applications in fish feed formulations and disease prevention.

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Screening of Lactic Acid Bacteria from the Gut of Chrysichthys nigrodigitatus for Use as Probiotics in Aquaculture Production

Cited by 3 publications

References 3 publications

Fish Gut Microbiome: Current Approaches and Future Perspectives

Fish Gut Microbiome: Current Approaches and Future Perspectives

In Vitro and In Silico Analysis of the Gut Microbiome of Zebrafish for Bioremediation Approach of Zinc-Contaminated Aquatic Environments

Effect of Dietary Supplementation of Bacillus subtilis and Terribacillus saccharophillus on Innate Immune Responses of a Tropical Freshwater Fish, Labeo rohita

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