Screening of Isochrysis strains for simultaneous production of docosahexaenoic acid and fucoxanthin

Sun, Zheng; Wang, Xiaofei; Liu, Jin

doi:10.1016/j.algal.2019.101545

Cited by 57 publications

(66 citation statements)

References 36 publications

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“…The enzymatic pre-treatment did not improve the PUFA extraction (34.1% to 37.7%), but higher PUFA contents were obtained than traditional methods. Other researchers also found a similar fatty acid composition [ 41 , 42 ].…”

Section: Resultssupporting

confidence: 61%

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

2020

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Microalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid extraction (PLE) was optimized and compared with traditional lipid extraction methods, Folch and Bligh and Dyer, and with a new Ultrasound Assisted Extraction (UAE) method for lipids from microalgae Isochrysis galbana. To further optimize PLE and UAE, enzymatic pre-treatment of microalga Isochrysis galbana was studied with commercial enzymes Viscozyme and Celluclast. No significant differences were found for lipid yields among different extraction techniques used. However, advanced extraction techniques with or without pre-treatment are a green, fast, and toxic solvent free alternative to traditional techniques. Lipid composition of Isochrysis was determined by HPLC-ELSD and included neutral and polar lipids, showing that each fraction comprised different contents in omega-3 polyunsaturated fatty acids (PUFA). The highest polar lipids content was achieved with UAE (50 °C and 15 min) and PLE (100 °C) techniques. Moreover, the highest omega-3 PUFA (33.2%), eicosapentaenoic acid (EPA) (3.3%) and docosahexaenoic acid (DHA) (12.0%) contents were achieved with the advanced technique UAE, showing the optimized method as a practical alternative to produce valuable lipids for food and nutraceutical applications.

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Section: Resultssupporting

confidence: 61%

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

2020

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“…Hence, semi-continuous and fed-batch strategies have been extensively applied to avoid the limitation or inhibition of substrates during microalgal cultivation. The productivities of DHA and fucoxanthin of Isochrysis strains CCMP1324 were significantly enhanced in a semi-continuous strategy [27]. Further, a semi-continuous cultivation strategy with urea limitation was applied to improve lipid production in Chlorella sp.…”

Section: Cultivation Strategies Based On Nutrient Conditionsmentioning

confidence: 99%

“…For example, in N. oceanica , the level of total lipids and secondary carotenoids (e.g., β-carotene, zeaxanthin, astaxanthin, and canthaxanthin) significantly increased under high light condition, when compared to that of the low light condition [ 68 ]. Additionally, the highest content of DHA and fucoxanthin were obtained at a low light intensity of 30 μmol/m 2 /s, and declined with the increase in light intensity to 120 μmol/m 2 /s in Isochrysis [ 27 ].…”

Section: Co-production Of Multiple Compounds In Marine Microalgae mentioning

confidence: 99%

“…Sufficient nitrogen is essential for cell growth and the accumulation of primary metabolites, while it is not suitable for the biosynthesis of secondary metabolites and storage lipids and carbohydrates. It was found that fucoxanthin and DHA contents enhanced with the increase in nitrate concentration from 25 to 100 mg/L in Isochrysis strain CCMP1324 [ 27 ]. Similarly, phycoerythrin content was boosted when nitrate concentration was increased from 800 to 1100 and 1320 μΜ in Rhodomonas sp.…”

Section: Co-production Of Multiple Compounds In Marine Microalgae mentioning

confidence: 99%

“…It is commonly recognized that sufficient phosphorus is required for cell growth and production of primary metabolites in microalgae because increasing phosphorus assimilation is beneficial for the biosynthesis of nucleic acids and phospholipids, and may further affect the accumulation of most primary metabolites [ 95 ]. For instance, the contents of DHA and fucoxanthin enhanced with the increase in phosphorus concentration from 1.13 to 4.5 mg/L in the Isochrysis strain CCMP1324 [ 27 ]. Carbohydrate content was found to be increased with the rise in phosphorus concentrations from 0 to 120 mg/L in Rhodosorus sp.…”

Section: Co-production Of Multiple Compounds In Marine Microalgae mentioning

confidence: 99%

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Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

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Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao

Sá

Itd

et al. 2020

Biotech & Bioengineering

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The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L−1) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R2 for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L−1: 75.66 and 98.14 mg L−1 d−1, for T. lutea and P. tricornutum, respectively; 0.4 g L−1: 129.9 and 158.47 mg L−1 d−1, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L−1: 1.14 and 1.41 mg L−1 d−1, T. lutea and P. tricornutum; 0.4 g L−1: 2.09 and 1.73 mg L−1 d−1, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

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Screening of Isochrysis strains for simultaneous production of docosahexaenoic acid and fucoxanthin

Cited by 57 publications

References 36 publications

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

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