Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol.

Balogun, S. A.; Shofola, T. C.; Okedeji, A. O.

doi:10.30955/gnj.001514

Cited by 5 publications

References 30 publications

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Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

Mohammed,

Omar Zrary,

Hasan

2023

Biologia

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Petroleum hydrocarbons are a major concern due to their widespread distribution in the environment, and their harmful effects on humans. The use of biodegradation methods to clean soil that has been contaminated by spilt petroleum can be an effective remediation strategy. Therefore, in this study, the presence of indigenous microorganisms in soil contaminated with crude oil was investigated and their ability to degrade petroleum in vitro was studied. Three methods were used to detect crude oil and pure hydrocarbon degradation by bacterial and fungal isolates. Spectrophotometric method, 2,6-Dichlorophenol indophenol (DCPIP) method and hydrocarbon analysis was performed by GC–MS. Furthermore, PCR was carried out to detect the genes AlkB and PMO in the microbial communities. Seventeen bacterial isolates and 5 fungal isolates were recovered from 19 soil culture samples obtained from three different oil-contaminated sites at Taq-taq (TTOPCO) oil field and from a control site without oil contamination. The bacterial samples were identified culturally and morphologically, and the results were validated by genetic analysis. The results of this study revealed that the indigenous bacterial and fungal isolates had a high capacity to degrade crude oil and pure hydrocarbon fractions. Our results provide insight into the great abilities of Bacillus anthracis, Bacillus cereus, Achromobacter sp., Aspergillus lentulus and Rhizopus arrhizus to degrade crude oil and pure hydrocarbon fractions, and these data are supported by the presence of the PMO and AlkB genes in the isolated microorganisms.

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Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

Mohammed,

Omar Zrary,

Hasan

2023

Biologia

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Electron shuttles stimulate the reductive dissolution of jarosite by Acidiphilium cryptum

et al. 2020

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Bioremediation of Cutting Pits by Autochtonous Bacteria-Fungi Consortia

Carpani

Pietrini

Baric

et al. 2021

Day 2 Tue, November 16, 2021

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The aim of this work is to verify the potential of a consortium of autochthonous bacteria and fungi, isolated from samples of contaminated soils and water collected in a site containing cutting pits muds, in order to evaluate enhancing in biodegradation of hydrocarbons content. This innovative technique would take advantage of the synergistic effect of bacteria and fungi. In addition, this technique would allow to avoid the introduction of commercial allochthonous microflora for soil remediation and the use of chemical products for tool cleaning. Samples retrieved from a production site were used to isolate bacterial and unicellular fungal species able to grow on hydrocarbons were demonstrated to be able to degrade light and "diesel-like" hydrocarbons under laboratory conditions and in liquid cultures in less than a month. The activity of the consortium was also tested on crude oil, showing an overall degradation of the analyzable fraction greater of sixty percent after a 14-day incubation. Low C number linear hydrocarbons were the preferred substrate, but also cycloalkanes and mono- and di-aromatics seemed to be a good growth substrate. Probably, the action of enzymes secreted by fungal strains could enhance the degradation of complex molecules such as polycyclic aromatic hydrocarbons. Lab tests of consortium efficiency on mud samples are ongoing and an on-site pilot test is foreseen, to prove the activity of the consortium under the challenging field conditions. The development of fungal and bacterial consortia for degradation of complex hydrocarbon mixtures will represent an innovative approach that combines the action of enzymes secreted by fungi followed by the bacterial breakdown, a synergistic effect which could potentially increase the rate and effectiveness of hydrocarbons decontamination.

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Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol.

Cited by 5 publications

References 30 publications

Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

Electron shuttles stimulate the reductive dissolution of jarosite by Acidiphilium cryptum

Bioremediation of Cutting Pits by Autochtonous Bacteria-Fungi Consortia

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