Screening of Fungal Azole Resistance in Different Environmental Samples

Pena, Pedro; Morais, Joana; Caetano, Liliana Aranha; Viegas, Carla

doi:10.1016/b978-0-12-819990-9.00017-2

Cited by 2 publications

(2 citation statements)

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“…The use of passive sampling methods to assess exposure, in what concerns the microbial contamination, allows to overcome the expected fluctuation due to a wide range of factors such as humidity levels, ventilation, human occupancy and their activities, environmental characteristics, water infiltrations, and outdoor air [ 19 , 20 ]. Additionally, in this specific setting, soil can be brought inside the workers’ facilities increasing the microbial contamination indoors [ 13 , 14 , 15 ]. The obtained results also followed the trend obtained in different studies where different sampling methods and culture media were employed.…”

Section: Discussionmentioning

confidence: 99%

“…The emergence of fungal resistance to medical antifungal agents (mostly azoles) and the increasing incidence of azole-resistant disease due to resistant Aspergillus fumigatus originating from the environment have been reported [ 12 , 13 , 14 , 15 ]. This opportunistic fungus is responsible for severe diseases such as invasive aspergillosis in humans, with reserved prognostic in immunocompromised individuals.…”

Section: Introductionmentioning

confidence: 99%

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Six Feet under Microbiota: Microbiologic Contamination and Toxicity Profile in Three Urban Cemeteries from Lisbon, Portugal

Viegas

Cervantes

Dias

et al. 2022

Toxins

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Cemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled dust, settled dust filters and electrostatic dust cloths—EDC) were employed. The molecular detection of Aspergillus sections and SARS-CoV-2, as well as mycotoxin analysis, screening of azole resistance, and cytotoxicity measurement were also conducted. Total bacteria contamination was 80 CFU·m−2 in settled dust samples, reached 849 CFU·m−2 in EDC and 20,000 CFU·m−2 in swabs, and ranged from 5000 to 10,000 CFU·m−2 in filters. Gram-negative bacteria (VRBA) were only observed in in settled dust samples (2.00 × 105 CFU·m−2). Regarding Aspergillus sp., the highest counts were obtained in DG18 (18.38%) and it was not observed in azole-supplemented SDA media. SARS-CoV-2 and the targeted Aspergillus sections were not detected. Mycophenolic acid was detected in one settled dust sample. Cytotoxic effects were observed for 94.4% filters and 5.6% EDC in A549 lung epithelial cells, and for 50.0% filters and 5.6% EDC in HepG2 cells. Future studies are needed in this occupational setting to implement more focused risk management measures.

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Section: Discussionmentioning

confidence: 99%