Object Moringa oleifera has numerous medicinal values and uses worldwide. Moringa oleifera is applied to remedy numerous diseases like malaria, arthritis, parasitic diseases, typhoid fever, swelling, cuts, and diseases of the skin, diabetes, and hypertension. However, no scientific evidence is available about the cardio-tropic activity of Moringa oleifera. The current research was carried out to investigate the cardio-tropic activity of hydro-alcoholic extract of Moringa oleifera leaves on the isolated heart of the Albino Wistar rat. The Moringa oleifera leaves were collected, washed, shade dried, and extracted with 70% ethanol. A stock solution (100µg/ml) was prepared by using distilled water. The cardio-tropic activity was screened on the isolated rat heart at a dose of 0.1ml, 0.2ml, and 0.4ml of 70% ethanolic extract of the Moringa oleifera leaf. Acetylcholine (0.0125ml, 0.0250ml, 0.0500ml, 0.1000ml) and adrenaline (0.05ml, 0.1ml, 0.2ml, 0.4ml) were used as standard drugs. Also, on the isolated rat heart, the dose-response curve of 70% ethanolic extract of the Moringa oleifera leaf and the standard drugs were screened at the above same dose. The 70% ethanolic extract of Moringa oleifera leaf (100µg/ml) has produced significant negative cardio-tropic activity as compared to standard drugs. The study indicates that 70% ethanolic extract of Moringa oleifera leaf shows substantial negative cardio-tropic activity on the isolated rat heart in dose dependent manner.