Screening of differentially expressed proteins in placentas from patients with late‐onset preeclampsia

Abstract

Purpose: Preeclampsia (PE) is a severe disease that endangers the safety of mothers and fetuses worldwide. In the absence of specific treatments, more studies on novel predictive and diagnostic biomarkers for PE are required.Experimental design: Data-independent acquisition proteomics, with five biological replicates, was used to investigate the protein expression profiles of placental tissues from patients with PE and normal pregnant women.Results: In total, 52 differentially expressed proteins (DEPs) were identified, 34 of them were upregulated and 18 downregulated. Bioinformatics analyses revealed that PE was associated with multiple GO terms and KEGG pathways. Arginase-1 (ARG1), ferritin light chain (FTL), and RNA cytidine acetyltransferase (NAT10) were identified as hub proteins, which were further validated in placental tissues and maternal plasma by western blot and ELISA.Conclusions and clinical relevance: FTL expression was significantly lower in the placental tissues and early and late pregnancy plasma of patients with PE compared to that in normal pregnant women. This study is the first to propose that FTL may be a potential predictive and diagnostic biomarker for PE; it provides a proteomics insight for understanding the pathological mechanism of this disease.

Ferritin light chain deficiency-induced ferroptosis is involved in preeclampsia pathophysiology by disturbing uterine spiral artery remodelling

“…1 A and B). The demographic data of the study population have been previously described in detail [ 24 ]. According to the CytoHubba plug-in MCC algorithm in Cytoscape software, the top three proteins with high core degrees were analysed ( Fig.…”

“…Placental tissues from patients with PE (n = 4) and normal pregnant women (n = 5) were used for proteomics analysis. The detailed method of proteomics identification has been previously described [ 24 ]. Finally, the R package MSstats was used to evaluate significant differences in protein expression, and the genes with fold change >1.5 and p-value < 0.05 were identified as differentially expressed proteins (DEPs).…”

Placental and plasma early predictive biomarkers for gestational diabetes mellitus

“…Placental tissue samples from the GDM (n = 5) and control (n = 5) groups were processed for DIA. Protein extraction was performed as described previously [13]. In brief, the samples were mixed with a protease inhibitor cocktail (Sigma, USA), SDSL3 (Beijing Genomics Institute, China) in a solution containing EDTA (BBI, USA).…”

Quantitative Proteomic Analysis of Serum Reveals MST1 as a Potential Candidate Biomarker in Spontaneously Diabetic Cynomolgus Monkeys

“…Furthermore, a data-independent acquisition (DIA)-based quantitative proteomics technology has received much attention in the recent years as it is a new tandem mass spectrometry method that fragments and analyzes all peptide ions within a selected mass-to-charge ratio range, even those with a low abundance. It also has significant advantages for throughput, quantitative accuracy, and reproducibility, which have been applied in research studies on biomarkers and basic and clinical trials for various diseases. − The serum is the most complex clinical specimen that can be easily obtained, and most proteins secreted by cells are finally released into the blood circulation . Therefore, serum may contain fundamental information related to the pathophysiological status of all tissues and organs.…”

“…It also has significant advantages for throughput, quantitative accuracy, and reproducibility, which have been applied in research studies on biomarkers and basic and clinical trials for various diseases. 11 − 13 The serum is the most complex clinical specimen that can be easily obtained, and most proteins secreted by cells are finally released into the blood circulation. 9 Therefore, serum may contain fundamental information related to the pathophysiological status of all tissues and organs.…”