Screening of differentially expressed genes associated with Kawasaki disease by microarray analysis

Jiang, Jie; Cai, Yi; Li, Zhuoying; Huang, Lihua; Chen, Jia; Tian, Li; Wu, Zhixiang; Li, Xin; Chen, Zhiheng; Chen, Chun-Yuan; Yang, Zhangping

doi:10.3892/etm.2017.4907

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…Although there were a number of differentially regulated targets, autoantibodies to EDIL3 were most clearly overexpressed in Kawasaki disease patients as compared to MIS-C ( Figure 7 H). The gene encoding this protein has previously been associated with Kawasaki disease ( Jiang et al., 2017 ), and the target protein is a structural glycoprotein in arterial vessel walls that is regulated in response to vascular injury. One function of EDIL3 is to inhibit inflammatory cell recruitment and extravasation across the endothelium, and when EDIL3 is disrupted in mice, there is increased tissue infiltration of neutrophils and elevated inflammatory responses ( Choi et al., 2008 ).…”

Section: Resultsmentioning

confidence: 99%

The Immunology of Multisystem Inflammatory Syndrome in Children with COVID-19

Consiglio

Cotugno

Sardh

et al. 2020

Cell

772

950

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is typically very mild and often asymptomatic in children. A complication is the rare multisystem inflammatory syndrome in children (MIS-C) associated with COVID-19, presenting 4–6 weeks after infection as high fever, organ dysfunction, and strongly elevated markers of inflammation. The pathogenesis is unclear but has overlapping features with Kawasaki disease suggestive of vasculitis and a likely autoimmune etiology. We apply systems-level analyses of blood immune cells, cytokines, and autoantibodies in healthy children, children with Kawasaki disease enrolled prior to COVID-19, children infected with SARS-CoV-2, and children presenting with MIS-C. We find that the inflammatory response in MIS-C differs from the cytokine storm of severe acute COVID-19, shares several features with Kawasaki disease, but also differs from this condition with respect to T cell subsets, interleukin (IL)-17A, and biomarkers associated with arterial damage. Finally, autoantibody profiling suggests multiple autoantibodies that could be involved in the pathogenesis of MIS-C.

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Section: Resultsmentioning

confidence: 99%

The Immunology of Multisystem Inflammatory Syndrome in Children with COVID-19

Consiglio

Cotugno

Sardh

et al. 2020

Cell

772

950

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“…The present study also predicted that the selected miRNAs may be involved in KD by regulating the inflammatory target genes expressed in PBMCs. CXCR1 was one of the targets and previous studies have reported that IL-8 and its receptors, CXCR1 and C-X-C chemokine receptor type 2, were upregulated in patients with KD ( 46 ) and in coronary artery diseases ( 47 ). By binding to CXCR1, IL-8 may promote the production of other inflammatory mediators through the activation of the p38-mitogen-activated protein kinase/extracellular signal-regulated kinase-nuclear factor (NF)-κB pathways ( 48 ), which may subsequently induce apoptosis in vascular endothelial cells, a potential mechanism for KD ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal miR‑328, miR‑575, miR‑134 and miR‑671‑5p as potential biomarkers for the diagnosis of Kawasaki disease and the prediction of therapeutic outcomes of intravenous immunoglobulin therapy

2018

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The present study was conducted to screen serum exosomal microRNAs (miRNAs) for the early diagnosis of Kawasaki disease (KD) and to investigate their underlying mechanisms by analyzing microarray data under accession numbers GSE60965 [exosomal miRNA, including three pooled serum samples from 5 healthy children, 5 patients with KD and 5 patients with KD following intravenous immunoglobulin (IVIG) therapy] and GSE73577 (mRNA, including peripheral blood mononuclear cell samples from 19 patients with KD prior to and following IVIG treatment) from the Gene Expression Omnibus database. Differentially expressed miRNAs (DE-miRNAs) and genes (DEGs) were identified using the Linear Models for Microarray data method, and the mRNA targets of DE-miRNAs were predicted using the miRWalk 2.0 database. The functions of the target genes were analyzed using the Database for Annotation, Visualization and Integrated Discovery (DAVID). As a result, 65 DE-miRNAs were identified with different expression patterns between the healthy children and patients with KD and between patients with KD and patients with KD following IVIG therapy. The target genes of 15 common DE-miRNAs were predicted. Following overlapping the target genes of DE-miRNAs with 355 DEGs, 28 common genes were identified and further screened to construct a network containing 30 miRNA-mRNA regulatory associations. Of these associations, only miR-328-spectrin α, erythrocytic 1, miR-575-cyclic AMP-responsive element-binding protein 5/b-1,4-galactosyltransferase 5/WD repeat and FYVE domain-containing 3/cystatin-A/C-X-C motif chemokine receptor 1/protein phosphatase 1 regulatory subunit 3B, miR-134-acyl-CoA synthetase long chain family member 1/C-type lectin domain family 1 member A and miR-671-5p-tripartite motif containing 25/leucine rich repeat kinase 2/kinesin family member 1B/leucine rich repeat neuronal 1 were involved in the negative regulation of gene expression. Functional analysis indicated that the identified target genes may be associated with inflammation. Accordingly, serum exosomal miR-328, miR-575, miR-134 and miR-671-5p may act as potential biomarkers for the diagnosis of KD and the prediction of outcomes of the IVIG therapy by influencing the expression of inflammatory genes.

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“…Various microarray based studies have been carried out to identify the genes associated with KD. Expression of these genes may be used as a novel diagnostic and prognostic biomarker for KD [51].…”

Section: Biomarkers In Kdmentioning

confidence: 99%

Kawasaki Disease

Pilania¹,

Singh²

2019

Periodic and Non-Periodic Fevers

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Kawasaki disease (KD) is an acute and usually self-limiting medium vessel vasculitis of childhood that has a predilection to involve the coronary arteries. It is characterized by the sequential appearance of a constellation of clinical features [1]. However, none of these clinical findings is, in itself, pathognomonic of KD. Many of these clinical features can, in fact, be seen in other common febrile illnesses of children. It is for this reason that the diagnosis of KD is often considered to be a clinical challenge [2]. Approximately 15-25% of untreated patients may go on develop coronary artery abnormalities (CAAs) and this remains the major cause of morbidity, and occasional mortality, in KD [3].

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Screening of differentially expressed genes associated with Kawasaki disease by microarray analysis

Cited by 7 publications

References 33 publications

The Immunology of Multisystem Inflammatory Syndrome in Children with COVID-19

The Immunology of Multisystem Inflammatory Syndrome in Children with COVID-19

Serum exosomal miR‑328, miR‑575, miR‑134 and miR‑671‑5p as potential biomarkers for the diagnosis of Kawasaki disease and the prediction of therapeutic outcomes of intravenous immunoglobulin therapy

Kawasaki Disease

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