Screening of Different Media and Substrates for Cultural Variability and Mass Culture ofArthrobotrys dactyloidesDrechsler

Abstract

Variability in growth and sporulation of five isolates of Arthrobotrys dactyloides was studied on five agar, 6 bran and 5 grain media. Potato dextrose agar (PDA) supported maximum growth of isolate A, C and E, while growth of isolate B and D was significantly lower on this medium. On Czapek's agar and yeast glucose agar media the differentiation in the isolates in relation to growth was poor than PDA. The other two media showed much poorer differentiation. On Czapek's agar medium, sporulation was recorded in isolate B only, whereas other isolates showed rare sporulation. Among the bran media, pea bran agar medium supported maximum growth of all the isolates except isolate B. Gram and rice bran agar media were next best. However, the growth of isolate B on the gram bran agar medium was more or less equal as other isolates. On pigeon pea bran agar medium, isolate E failed to grow while other isolates recorded poor growth. On lentil bran agar medium, only isolate B and D recorded little growth, whereas other isolates failed to grow. All the isolates recorded good sporulation on bran agar media except pigeon pea and lentil bran agar media. The grain agar media supported moderate to very good growth of all the isolates. In general isolate B remained slow growing on these media except gram grain and sorghum grain agar media on which growth of this isolate was comparable to other isolates. Sporulation in general, was good on all the grain agar media. Among different substrates screened, barley grain and pea bran were found superior to others for mass culture of isolate A of A. dactyloides.

Production of Blastospore of EntomopathogenicBeauveria bassianain a Submerged Batch Culture

“…Kumar et al (2005) also demonstrated that corn grain medium supported the heavy sporulation of the fungus Arthrobotrys dactyloides . Corn meal has been generally considered a good substrate for fungal growth, has a high starch content (67.6~69.4%), and also contains other nutrients (Lee et al, 2008).…”

“…After 5 days of incubation, the maximal blastospore yield (5.59 × 10 8 spores/ ml ) was achieved with a medium containing 3% corn meal, 2% CSP, and a supplement of 1% wheat bran. Brans of various crops are agricultural wastes and were utilized as the microbial culture medium (Jatinder et al, 2006; Kim et al, 1977; Kumar et al, 2005). In a previous study, among a variety of tested brans, including the brans of pea, wheat, rice, gram, pigeon pea, and lentil, the maximum sporulation of various strains of the fungus Arthrobotrys dactyloides was achieved in rice bran medium (Kumar et al, 2005).…”

“…Brans of various crops are agricultural wastes and were utilized as the microbial culture medium (Jatinder et al, 2006; Kim et al, 1977; Kumar et al, 2005). In a previous study, among a variety of tested brans, including the brans of pea, wheat, rice, gram, pigeon pea, and lentil, the maximum sporulation of various strains of the fungus Arthrobotrys dactyloides was achieved in rice bran medium (Kumar et al, 2005). Rice bran has also been employed as a sole nutrition source for the culturing of Streptomyces actuosus (Wang, et al, 2003) and Lactobacillus brevis (Ohtomo et al, 2006).…”

“…Kumar et al (2005) also mentioned that the poor growth of the fungus Arthrobotrys dactyloides on wheat bran might be attributed to its higher concentrations. Similar observations regarding the reduced growth of Catenaria anguillulae at higher concentrations of wheat bran in medium and mass culture were made by Vaish and Singh (2002).…”

Genomic Insights into the Adaptation to Parasitism in Nematode-Trapping Fungi and Transcriptomics during Infection of Caenorhabditis Briggsae and Plant-Parasitic Nematodes

“…Application of mass culture of Arthrobotrys dactyloides and Dactyalria brochopaga grown on barley substrates by method developed by Kumar et al (2005) was found effective against the root-knot disease of tomato and rice. The number of root knots, females, egg masses and second stage juveniles of root knot nematodes were found to reduce by the application of colony forming units of A.datyloides and D.brochopaga in tomato and rice (Kumar and Singh, 2006;Singh et al, 2007).…”