Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina

Sauka, Diego Herman; Cozzi, Jorge; Benintende, Graciela Beatriz

doi:10.1007/s10482-005-3368-2

Cited by 15 publications

(24 citation statements)

References 11 publications

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“…1). The combination of cry2Aa/cry2Ab genes were the most frequent, followed by cry2Aa and cry2Ab alone, and a novel cry2-type gene alone was only in JF19-2 (Table 1), which was conformed to the distribution features of Argentina (19). But Ben-Dov et al (3) found that Bt isolates from Israel, Kazakhstan and Uzbekistan containing cry2Ab alone were the most frequent, followed by cry2Aa/cry2Ab and cry2Ab/cry2Ac, which revealed that the distribution of cry2 genes in Sichuan basin was unique compare with other countries of Asian.…”

Section: Discussionsupporting

confidence: 56%

“…2B), which suggests that these strains may be promising Bt resource for the controlling of pests. Specially, we detected the insecticidal crystal protein genes harbored in strain JF19-2 by using all the primers based on the conserved regions of each class of cry and cyt genes as previously described by Sauka et al (19) and Su (22). But only one PCR product about 1.5 kb was obtained using the primers II (+)/II (-) (data not shown), which revealed that this strain may contain only one cry2 gene.…”

Section: Discussionmentioning

confidence: 84%

“…But no systematic research about cry2 genes in China or basins was done, so it is of interest to determine the distribution feature of cry2 genes and identify the type of cry2 genes. In this paper, the presence of cry2-type genes was analyzed in 791 Bt isolates from different regions and vegetation in Sichuan basin as described by Sauka et al (19). The rates of strains containing cry2-type gene from different vegetation and ecological region were different (Table 1), which revealed that the distribution of these strains are diversity in different typically ecological regions.…”

Section: Discussionmentioning

confidence: 95%

“…Many PCR-based methods have been developed to detect cry2-type genes (3; 14; 24). Sauka et al (19) have established a PCR-RFLP method to describe the distribution of cry2-type genes profiles from Argentina and one novel cry2-type gene was found in their collections. However, to our knowledge, there was only two work described the distribution of cry2 genes of China (24; 25) and no systematic research on the type of cry2 genes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China

Liang¹,

Liu²,

Zhu³

et al. 2011

Braz. J. Microbiol.

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Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate (JF19-2). The full-length of this novel gene was obtained by the method of thermal asymmetric interlaced PCR (Tail-PCR), which was designated as cry2Ag1 (GenBank No. ACH91610) by the Bt Pesticide Crystal Protein Nomenclature Committee. In addition, the result of scanning electron microscopic (SEM) observation showed that these strains had erose, spherical, bipyramidal, and square crystal. And the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that these strains harbored about one to three major proteins.These strains exhibited a wide range of insecticidal spectrum toxic to Aedes aegypti (Diptera) and Pieris rapae Linnaeus, 1758 (Lepidoptera). Particularly, JF19-2 contained cry2Ag gene had the highest insecticidal activity. All these researches mentioned above revealed the diversity and particularity of cry2-type gene resources from Bacillus thuringiensis strains in Sichuan basin.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China

Liang¹,

Liu²,

Zhu³

et al. 2011

Braz. J. Microbiol.

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“…In order to determine if toxic B. thuringiensis strains posses new cry-type genes, genomic DNA of the strains was amplified with cry2A forward and cry2A reverse universal primers as described by Sauka et al (2005). This primer pair results in a PCR product at around 1556 bp.…”

Section: Partial Dna Sequence Analysis For Cry2 Gene Of Toxic Bacillumentioning

confidence: 99%

Bioactivities of cry gene positive Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) strains on Ephestia kuehniella Zeller, 1879 and Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae)

Alper

Güneş

Çöl

et al. 2016

Turkish Journal of Entomology

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Selection and characterization of Bacillus thuringiensis strains from northwestern Himalayas toxic against Helicoverpa armigera

2017

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In this study, we present the selection and the characterization of Bacillus thuringiensis (Bt) strains with respect to their cry/cyt gene content and toxicity evaluation toward one of the most important polyphagous lepidopteran pest, Helicoverpa armigera. Fifty‐six Bt isolates were obtained from 10 different regions of northwestern Himalayas, recording a total B. thuringiensis index of 0.62. Scanning electron microscopy revealed presence of bipyramidal, spherical, flat and irregular crystal shapes; SDS‐PAGE analysis of spore‐crystal mixtures showed the prominence of 130, 70, and 100 kDa protein bands in majority of the isolates; PCR analysis with primers for eight cry and cyt gene families and 13 cry gene subfamilies resulted in isolates showing different combinations of insecticidal genes. Strains containing cry1 were the most abundant (57.1%) followed by cyt2 (46.42%), cry11 (37.5%), cry2 (28.57%), cry4 (21.42%), cyt1 (19.64%), cry3 (8.9%), and cry7, 8 (7.14%). A total of 30.35% of the strains did not amplify with any of the primers used in this study. Median lethal concentration 50 (LC50) estimates of spore‐crystal mixtures of Bt‐JK12, 17, 22, 48, and 72 against second instar larvae of H. armigera was observed to be 184.62, 275.39, 256.29, 259.93 μg ml−1, respectively. B. thuringiensis presents great diversity with respect to the presence of crystal protein encoding genes and insecticidal activity. Four putative toxic isolates identified in this study have potential application in insect pest control. B. thuringiensis isolate JK12 exhibited higher toxicity against H. armigera than that of B. thuringiensis HD1, hence can be commercially exploited to control insect pest for sustainable crop production. The results of this study confirm the significance of continuous exploration of new Bt stains from different ecological regions of the world.

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Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina

Cited by 15 publications

References 11 publications

Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China

Characterization of cry2-type genes of Bacillus thuringiensis strains from soil-isolated of Sichuan basin, China

Bioactivities of cry gene positive Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) strains on Ephestia kuehniella Zeller, 1879 and Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae)

Selection and characterization of Bacillus thuringiensis strains from northwestern Himalayas toxic against Helicoverpa armigera

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