Screening of chilli pepper ( Capsicum spp.) lines for resistance to the begomoviruses causing chilli leaf curl disease in India

Srivastava, Arpita; Mangal, Manisha; Saritha, R. K.; Kalia, Pritam

doi:10.1016/j.cropro.2017.06.015

Cited by 37 publications

(28 citation statements)

References 22 publications

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“…The previously published resistant lines PBC 142, PBC 143, PBC 145, PBC 148, PBC 149, PBC 495 (Kenyon et al, 2014a;Srivastava et al, 2015Srivastava et al, , 2017 were not resistant in our study ( Table 2). The reasons for these differences could be attributable to the use of different species of Begomovirus, with Tomato yellow leaf curl Thailand virus was used in Taiwan (Kenyon et al, 2014a) and Chili leaf curl virus (ChiLCV), Tomato leaf curl Joydebpur virus (ToLCJoV) (monopartite), and Tomato leaf curl New Delhi virus under open field conditions were used in India (Srivastava et al, 2015(Srivastava et al, , 2017. The differences could also be due to inoculation techniques used (viruliferous whitefly or natural field inoculation) or because we scored at 120 dpi and not at the seedling stage.…”

Section: Resultssupporting

confidence: 54%

“…glabriusculum, four (VI012478, VI102479, VI012528, and VI014924) were C. baccatum, 14 (PBC 206, PBC 506, PI 152225, PI 164561, PI 159234, PI 159236, PI 175622, VI012270, VI012271, VI012596, VI012642, VI012665, VI012668, and VI029244) were C. chinense, one (PI 566812) was C. chacoense, one (PBC 687) was C. frutescens, and the rest (76) were C. annuum. Selection of entries for screening was based on resistance reported in publications (Adluri et al, 2017;Kenyon et al, 2014a;Srivastava et al, 2015Srivastava et al, , 2017, historical breeding notes, and personal communications with private and public sector plant breeders working with the disease.…”

Section: Methodsmentioning

confidence: 99%

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A Novel Source of Resistance to Pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in Chile Pepper

Barchenger¹,

Yule²,

Jeeatid³

et al. 2019

horts

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Chile pepper (Capsicum annuum L.) is an increasingly important vegetable and spice crop. Among the most devastating chile pepper–infecting viruses, especially in tropical and subtropical regions, are members of the whitefly transmitted Begomovirus, which cause pepper yellow leaf curl (PYLC). An effective PYLC management strategy is the development of resistant cultivars. However, genetic recombination, acquisition of extra DNA components, and synergistic interactions among different begomoviruses have resulted in the rapid emergence of new viruses that can infect new hosts, cause new disease symptoms, and overcome host resistance. In this project, 98 Capsicum entries comprising breeding lines, open pollinated varieties, genebank accessions, and wild species were screened for resistance to strains of Pepper yellow leaf curl Thailand virus (PepYLCThV). We used a randomized complete block design with three replications and 10 plants per replication in field net-houses at two locations (Khon Kaen and Kamphaeng Saen, Thailand) using augmented inoculation by viruliferous whiteflies. Scoring was done at ≈60, 90, and 120 days after inoculation using a standardized 6-point scale (1 = no symptoms to 6 = very severe symptoms), and the average of the scores of 10 plants within each replication was used for analysis. Although no entry was immune to the disease, the breeding line 9852-123 was highly resistant. Several accessions and lines were moderately resistant at both locations, although a high level of variability within these entries was observed. Overall, the disease severity at the Khon Kaen location was greater compared with Kamphaeng Saen, highlighting the importance of multilocation testing for disease resistance. The resistant entry identified here can be used to study gene action and to move resistance genes into well-adapted germplasm.

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Section: Resultssupporting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

A Novel Source of Resistance to Pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in Chile Pepper

Barchenger¹,

Yule²,

Jeeatid³

et al. 2019

horts

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“…Identification of resistance sources is of utmost importance in any resistant breeding program. Keeping this in mind, natural field screening seemed best to eliminate the genotypes, which showed visible susceptible reaction under natural epiphytotic conditions [35]. The success of disease resistance breeding depends on the genetic variability and the reliable evaluation tests employed for identification of the resistant sources [36].…”

Section: Discussionmentioning

confidence: 99%

“…Kumar et al [39] screened 321 chilli genotypes under field conditions and identified only four genotypes with a highly tolerant reaction against ChiLCV. Whereas, the sixty germplasm lines of Capsicum annuum L., one each of C. chinense, C. chacoense and C. baccatum and two of C. frutescens were screened and, none of the genotypes was found to be free from the disease [35].…”

Section: Discussionmentioning

confidence: 99%

Appraisal of Genetics and Heterosis of Important Traits in Chilli Pepper Cultivated under the Influence of Chilli Leaf Curl Virus Disease

Vijeth¹,

Sreelathakumary²,

Rafeekher³

et al. 2019

Preprint

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Chilli leaf curl virus (ChiLCV) is a severe threat to the successful cultivation of the chilli peppers. Besides, the genetics and heterosis for important traits under the influence of ChiLCV infestation is not well studied. Therefore here, were crossed seven genotypes (lines) with high yield and quality attributes with four highly resistant genotypes (testers) in a line (L) × tester (T) mating design to produce 28 F1 hybrids. Parents and their hybrids were evaluated for yield attributes and ChiLCV resistance. All the four testers were symptomless and among seven lines, two were moderately resistant and five were moderately susceptible to the ChiLCV. Among 28 F1 hybrids, 12 showed a moderate resistant reaction, 11 were moderately susceptible and five susceptible to the ChiLCV disease. Based on per se performance, most promising hybrid combinations were L3 × T2, L6 × T1, and L3 × T1 for yield attributes. Whereas the superior crosses for ChiLCV resistance based on heterobeltosis, standard heterosis and specific combining ability test (SCA) effects were L6 × T1 and L7 × T3. Lines and testers showed significant differences for all the characters except for the plant height. The general combining ability (GCA) effects for testers were significant for all the traits except for days to first harvest. The ratio of σ2GCA/σ2SCA indicated the predominance of non-additive gene effects in the inheritance of traits studied in this experiment. The contribution of lines was more compared to testers for all the characters except for primary branches plant-1. Overall, this study provides valuable information regarding the genetics and heterosis in chilli with respect to ChiLCV infestation

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“…The typical symptoms under field conditions include upward curling, puckering, smaller leaves, stunted stems, and lack of fruiting. Furthermore, several other begomoviruses including Chilli leaf curl India virus, Tomato leaf curl Joydebpur virus and Tomato leaf curl New Delhi virus (ToLCNDV) have been found to co-infect the pepper plants along with ChLCV under field conditions (Khan et al, 2006 ; Fortes et al, 2016 ; Srivastava et al, 2017 ). In fact, synergistic interactions between different begomoviruses infecting pepper have been reported to cause breakdown of natural resistance in the host plant (Singh et al, 2016 ; Al-Shihi et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Conservation and Dispersion of Genes Conferring Resistance to Tomato Begomoviruses between Tomato and Pepper Genomes

et al. 2017

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In the present climate change scenario, controlling plant disease through exploitation of host plant resistance could contribute toward the sustainable crop production and global food security. In this respect, the identification of new sources of resistance and utilization of genetic diversity within the species may help in the generation of cultivars with improved disease resistance. Begomoviruses namely, Tomato yellow leaf curl virus (TYLCV) and Chilli leaf curl virus (ChLCV) are known to cause major yield losses in several economically important crop plants of the family Solanaceae. Though co-occurrence, association and synergistic interactions among these viruses in the host plants is reported, whether orthologous genetic loci in related host plants could be responsible for conferring resistance to these viruses has not been investigated yet. Several loci including Ty1, Ty2, Ty3, Ty4, and ty5 have been reported to confer resistance to leaf curl viruses in tomato. Here, we examined the pepper orthologous markers, corresponding to these QTL regions, for polymorphism between ChLCV susceptible and resistant genotypes of pepper. Further, to examine if the polymorphic markers are segregating with the disease resistance, Bulk Segregant Analysis (BSA) was performed on F2 population derived from crosses between resistant and susceptible lines. However, none of the markers showed polymorphism in BSA suggesting that the tested markers are not linked to genes/QTLs responsible for conferring resistance to ChLCV in the selected genotypes. In silico analysis was performed to study the synteny and collinearity of genes located within these QTL regions in tomato and pepper genomes, which revealed that more than 60% genes located in Ty2 and Ty4, 13.71% genes in Ty1, 23.07% in Ty3, and 44.77% genes located within ty5 QTL region in tomato are conserved in pepper genome. However, despite such a high conservation in gene content, the linkage relationship in these regions seems to be greatly affected by gross rearrangements in both the species.

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Screening of chilli pepper ( Capsicum spp.) lines for resistance to the begomoviruses causing chilli leaf curl disease in India

Cited by 37 publications

References 22 publications

A Novel Source of Resistance to Pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in Chile Pepper

A Novel Source of Resistance to Pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in Chile Pepper

Appraisal of Genetics and Heterosis of Important Traits in Chilli Pepper Cultivated under the Influence of Chilli Leaf Curl Virus Disease

Conservation and Dispersion of Genes Conferring Resistance to Tomato Begomoviruses between Tomato and Pepper Genomes

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