Screening of Cellulase and Pectinase by Using <i>Pseudomonas fluorescence</i> and <i>Bacillus subtilis</i>

Abstract

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10 -5 and 10 -6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

Isolation, Characterization of Zn Solubilizing Bacterium (Pseudomonas protegens RY2) and its Contribution in Growth of Chickpea (Cicer arietinum L) as Deciphered by Improved Growth Parameters and Zn Content

“…Bacillus, Enterobactor, and Pseudomonas genera are reported for the growth production of different crops like wheat, chickpea, mung, etc. 59 , 60 In literature, Pseudomonas spp. such as Pseudomonas fragi (EPS1), Pseudomonas fluorescens, and Pseudomonas aeruginosa have been extensively reported for their potential to plant growth and crop yield.…”

Isolation and Screening of Pectinolytic Bacterial Strains using Rotten Apples from Lahore, Pakistan

“…These isolates were then grown in modified MS media at 37°C for 24 hours in shaking condition for the production of extracellular pectinase [15]. Reetha et al (2014) reported that Pseudomonas fluorescens produced a higher level of pectinase enzyme as compared to Bacillus subtilis [16].…”

Growth Stimulation of Phaseolus vulgaris L Plantules by Strain Bacillus amyloliquefaciens Hla Producer of Beneficial Agricultural Enzymes

“…Pectinase is a group of enzymes that known to catalyse the pectic substance through depolymerisation and deexrification reaction. These enzymes have the role in preventing plant from infection caused by pathogen [11]. Protease, which can degrade cell wall proteins; and lipase, which can degrade some of the cell wall-associated lipid, all of which can to some extent individually lyse fungal cells [14].…”

“…The bacterium was streak on the medium and incubated at 30 ℃ for 48h. The appearance of a clear halo around growth indicated the presence of a pectinase [11].…”