Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection

Abstract

Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.

Analysis of the California list of pesticides, mycotoxins, and cannabinoids in chocolate using liquid chromatography and low‐pressure gas chromatography‐based platforms

“…In terms of phytoconstituents, multiple studies describing diverse analytical methods for the determination of cannabinoids and terpenes in cannabis matrices have been recently reported [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Furthermore, SMPRs for the quantitation of cannabinoids in cannabis concentrates have been published [ 38 ], and several literature reviews outlining the most up‐to‐date information in terpene and potency analysis are also available [ 7 , 8 , 39 , 40 ].…”

Recent forensic applications of enhanced chromatographic separation methods

“…In addition, enhanced separation methods in liquid chromatography and enhanced separation methods in supercritical fluid chromatography applicable to the forensic discipline from 2015 to 2020 will be reviewed. The applications discussed include arson investigations [13][14][15][16], environmental forensics [17,18], sexual assault investigations [19], toxicology [6,11,[20][21][22], and illicit drug analysis [5,7,8,21,[23][24][25][26][27][28].…”

Two‐dimensional hydrophilic interaction chromatography × reversed‐phase liquid chromatography for the preparative isolation of potential anti‐hepatitis phenylpropanoids from Salvia prattii

“…acidic) components in complex mixtures due to its high‐separation efficiency and relatively broader range of possible applications . Nevertheless, carrying out 2D HPLC is quite challenging due to the problem of finding column combinations that are sufficiently orthogonal for targeted components; this is a significant obstacle to the wider application of 2D HPLC . To the best of our knowledge, PPs are complex molecules usually possessing polar polyhydric aromatic ring coupled with one or more nonpolar phenylpropyl moieties, which showed great potential for retention and separation on HILIC and RPLC modes.…”