Screening of cancer tissue arrays identifies CXCR4 on adrenocortical carcinoma: correlates with expression and quantification on metastases using 64Cu-plerixafor PET
Abstract:Expression of the chemokine receptor CXCR4 by many cancers correlates with aggressive clinical behavior. As part of the initial studies in a project whose goal was to quantify CXCR4 expression on cancers non-invasively, we examined CXCR4 expression in cancer samples by immunohistochemistry using a validated anti-CXCR4 antibody. Among solid tumors, we found expression of CXCR4 on significant percentages of major types of kidney, lung, and pancreatic adenocarcinomas, and, notably, on metastases of clear cell ren… Show more
“…For CXCR4, suitable radioligands are available and have been successfully tested in lymphoproliferative malignancies (19)(20)(21). Two of these radioligands, 64 Cu-plerixafor and 68 Ga-Pentixafor, can also reliably assess the expression of CXCR4 in vivo in patients affected by ACC or aldosterone producing adenoma, respectively (4,38). Bluemel et al went a step further towards a potential theranostic use of CXCR4 and compared the performance of 68 Ga-pentixafor PET/CT with 18 F-FDG PET/ CT in 30 patients with advanced ACC (3).…”
Section: Discussionmentioning
confidence: 99%
“…They are an active component of the tumor microenvironment, driving tumor-specific immune responses and promoting invasion, metastasis, stemness and resistance to chemo-and radiotherapy (1,2). Recently, expression of CXCR4 was reported in primary tumors and metastatic lesions of patients with ACC both at protein level in vitro and in vivo using radiolabeled CXCR4 ligands (3,4).…”
Section: Introductionmentioning
confidence: 99%
“…Its ligand CXCL12 (SDF-1) is highly abundant in tissues that are common sites of metastasis such as lymph nodes, lung or bone, suggesting a specific chemokine-mediated trafficking-pattern of circulating tumor cells (6,9,10). CXCR7, an atypical chemokine receptor with a ten times higher affinity for CXCL12 compared to CXCR4, was detected at protein level in ACC metastases and correlated with CXCR4 expression (4). CXCR7 can generate CXCL12 gradients for CXCR4 but also acts as a CXCL12 "scavenger", as it is constantly recycled to the cell membrane after ligand binding (11,12).…”
“…For CXCR4, suitable radioligands are available and have been successfully tested in lymphoproliferative malignancies (19)(20)(21). Two of these radioligands, 64 Cu-plerixafor and 68 Ga-Pentixafor, can also reliably assess the expression of CXCR4 in vivo in patients affected by ACC or aldosterone producing adenoma, respectively (4,38). Bluemel et al went a step further towards a potential theranostic use of CXCR4 and compared the performance of 68 Ga-pentixafor PET/CT with 18 F-FDG PET/ CT in 30 patients with advanced ACC (3).…”
Section: Discussionmentioning
confidence: 99%
“…They are an active component of the tumor microenvironment, driving tumor-specific immune responses and promoting invasion, metastasis, stemness and resistance to chemo-and radiotherapy (1,2). Recently, expression of CXCR4 was reported in primary tumors and metastatic lesions of patients with ACC both at protein level in vitro and in vivo using radiolabeled CXCR4 ligands (3,4).…”
Section: Introductionmentioning
confidence: 99%
“…Its ligand CXCL12 (SDF-1) is highly abundant in tissues that are common sites of metastasis such as lymph nodes, lung or bone, suggesting a specific chemokine-mediated trafficking-pattern of circulating tumor cells (6,9,10). CXCR7, an atypical chemokine receptor with a ten times higher affinity for CXCL12 compared to CXCR4, was detected at protein level in ACC metastases and correlated with CXCR4 expression (4). CXCR7 can generate CXCL12 gradients for CXCR4 but also acts as a CXCL12 "scavenger", as it is constantly recycled to the cell membrane after ligand binding (11,12).…”
“…As in our study, expression differed in intensity and staining pattern but we obtained 82% of positive PT (93/113 animals). In human breast primary tumors, heterogeneity of expression pattern is often found and the positivity rate, is highly diverse [ 29 , 30 , 35 – 42 ]. This variability can be explained by the different IHC and scoring methods but also by the diversity of CXCR4 primary antibodies used.…”
BackgroundThe receptor CXCR4 and its ligand CXCL12 play crucial roles in breast cancer. Despite the fact that the spontaneous feline mammary carcinoma (FMC) is considered a suitable model for breast cancer studies, the importance of the CXCR4/CXCL12 axis in FMC is completely unknown. Therefore, this work aims to elucidate the role of CXCR4 and its ligand in the progression of FMC and metastatic disease.MethodsCXCR4 and CXCL12 expression was analyzed by immunohistochemistry and immunofluorescence on primary tumors (PT), regional and distant metastases of female cats with mammary carcinoma and correlated with serum CXCL12 levels, tumor molecular subtypes and clinicopathological features.ResultsCXCR4 was more expressed in PT than in metastases (p = 0.0067), whereas CXCL12 was highly expressed in metastatic lesions located in liver and lung (p < 0.0001), as reported for human breast cancer. Moreover, cats with CXCR4 positive PT exhibited significantly lower serum CXCL12 levels than cats with CXCR4 negative mammary carcinomas (p = 0.0324). At metastatic lesions, HER2-overexpressing tumors presented higher CXCR4 expression than the other molecular tumor subtypes (p = 0.012) and significant differences in overall (p = 0.0147) and disease-free survival (p = 0.0279) curves between the cats with CXCL12 positive and CXCL12 negative tumors were found. Indeed, CXCL12 negative PT were associated with unfavorable prognosis in cats with HER2-overexpressing tumors.ConclusionsThis work exposes part of the complex interaction between CXCR4 and CXCL12 in PT, but also in metastases of a breast cancer model. These findings could uncover novel therapeutic tools to be used in cats and humans.
“…Two promising directions that are being pursued in this regard are (1) the generation of radioactively-labeled small CXCR4 inhibitors for utilization in diagnostics and therapeutics of high CXCR4 expressing tumors [ 64 , 65 ], and (2) the usage of CXCR4 inhibitors to overcome tumor immune suppression in general and resistance to ICIs in particular [ 66 , 67 , 68 , 69 ]. With regards to the first direction, two groups have shown that labeling of the CXCR4 antagonist pentixafor with radioactive isotopes (68Ga and 64Cu) enabled the specific and accurate in vivo detection of high CXCR4 expressing tumors in the lung [ 64 , 65 ]. These findings are highly intriguing not only because they may, in the future, facilitate the selection of patients with high CXCR4 expressing tumors for anti-CXCR4 based therapeutics, but also because they open the way for labeling of CXCR4 antagonists with therapeutic radioactive isotopes such as 177Lu and 90Y for endoradiotherapy [ 70 ].…”
Section: Diagnostic and Therapeutic Potential Of Cxcr4 Antagonistsmentioning
Lung cancer is the second most common malignancy. Unfortunately, despite advances in multimodality therapeutics for the disease, the overall five-year survival rate among newly diagnosed lung cancer patients remains in the range region of 15%. In addition, although immune checkpoint inhibitors are increasingly being incorporated into lung cancer treatment protocols, the proportion of patients that respond to these agents remains low and the duration of response is often short. Therefore, novel methodologies to enhance the efficacy of immunotherapy in lung cancer are highly desirable. Chemokines are small chemotactic cytokines that interact with their 7 transmembrane G-protein–coupled receptors, to guide immune cell trafficking in the body under both physiologic and pathologic conditions. Tumor cells highjack a small repertoire of the chemokine/chemokine receptor system and utilize it in a manner that benefits local tumor growth and distant spread. The chemokine receptor, CXCR4 is expressed in over 30 types of malignant tumors and, through interaction with its ligand CXCL12, was shown exert pleotropic pro-tumorigenic effects. In this review, the pathologic roles that CXCL12/CXCR4 play in lung cancer propagation are presented. Furthermore, the challenges and potential benefits of incorporating drugs that target CXCL12/CXCR4 into immune-based lung cancer therapeutic protocols are discussed.
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