Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis

Monnerat, R. G.; Batista, A.; Medeiros, P. T.; Martins, E. S.; Melatti, V.; Praça, L. B.; Dumas, V. F.; Morinaga, Cristiane; Demo, C.; Gomes, Ana C.; Falcão, Rosana; Siqueira, C. B.; Silva-Werneck, Joseilde Oliveira; Berry, Colin

doi:10.1016/j.biocontrol.2006.11.008

Cited by 121 publications

(104 citation statements)

References 14 publications

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“…In this study, the toxic isolate Bt117-4 showed cuboidal crystals, which are known to contain proteins toxic to Lepidoptera (Dankocsik et al, 1990;Wu et al 1991;Monnerat et al, 2007;Silva-Werneck and Ellar, 2008).…”

Section: Discussionmentioning

confidence: 78%

“…Some research (Bobrowski et al, 2001;Silva et al, 2004;Monnerat et al, 2007;Silva-Werneck and Ellar, 2008;Gobatto et al, 2010) has shown the possibility of finding B. thuringiensis native isolates with toxicity to A. gemmatalis. The bioassays screening results with new B. thuringiensis isolates are useful for local and regional insect pest management.…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, Anticarsia gemmatalis Hübner 1818 (Lepidoptera, Noctuidae) is considered the key pest of soybean (Panizzi and Corrêa-Ferreira, 1997;Levy et al, 2004;Monnerat et al, 2007). A. gemmatalis, also known as velvetbean caterpillar, attack the aerial parts of plants (Gallo et al, 2002), hampering their development and thus causing losses in grain production (Morales et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Fiúza¹,

Schünemann

Pinto

et al. 2012

Braz. J. Biol.

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Bacillus thuringiensis is a bacterium used for biopesticides production and pest-resistant plants due to the synthesis of protein crystals by cry genes, which are effective in controlling several insect orders such as Lepidoptera. This work aimed at the evaluation and characterisation of two new B. thuringiensis isolates active against A. gemmatalis (Hübner 1818) larvae, which is the soybean major pest. The results showed that Bt117-4 isolate amplified fragments corresponding to cry2 and cry9 genes, and synthesised protein fragments equivalent to 130, 90 and 45 kDa. The Bt3146-4 isolate amplified DNA fragments corresponding to cry9 gene and synthesised protein fragments of 70, 58 and 38 kDa. Transmission electron microscopy revealed the presence of protein crystals in both isolates. CL50 with Cry purified proteins from Bt117-4 and Bt3146-4, corresponded to 0.195 and 0.191 µg larvae-1, respectively. The two B. thuringiensis isolates selected in this study were effective to control velvetbean caterpillar at laboratory conditions. Field tests should be carried on to develop new biopesticides formulation as well for cry genes resource for Anticarsia gemmatalis resistant transgenic plants.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Fiúza¹,

Schünemann

Pinto

et al. 2012

Braz. J. Biol.

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“…Thus, the LC 50 could not be estimated for isolate S1450. This isolate is in the kurstaki serotype described in the literature as toxic to insects of the order Lepidoptera (Monnerat et al, 2007) and causing 100% mortality of Agrotis ipsilon (Lepidoptera: Noctuidae). Additionally, cry1Aa, cry1Ab, cry1Ac, cry1, and cry2 genes were revealed by the molecular analysis, which confirmed the activity of the isolate against Lepidoptera (Menezes, Fiuza, Martins, Praça, & Monnerat, 2010).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)

et al. 2017

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ABSTRACT. Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae) is an insect pest of 60 economically important crops, including sugarcane, wheat, soybean, rice, beans, sorghum, peanuts, and cotton. The aim of this work was to select and characterize Bacillus thuringiensis isolates with insecticidal activity against E. Lignosellus that could be used as an alternative method of control. Selective bioassays were done to evaluate the toxicity of 47 isolates against first instar larvae of E. lignosellus. For the most toxic bacterial strains, the lethal concentration (LC 50 ) was estimated and morphological, biochemical and molecular methods were used to characterize the isolates. Among the 47 isolates tested, 12 caused mortality above 85% and showed LC 50 values from 0.038E+8 to 0.855E+8 spores mL -1. Isolates BR83, BR145, BR09, BR78, S1534, and S1302 had the lowest LC 50 values and did not differ from the standard HD-1 strain; the exception was BR83.The protein profiles produced bands with molecular masses of 60-130 kDa. The genes cry1, cry2, cry3, and cry11 were identified in the molecular characterization. The morphological analysis identified three different crystal inclusions: bipyramidal, spherical and cuboidal. Among the tested isolates, 12 isolates have potential for biotechnological control of E. Lignosellus by development of new biopesticides or genetically modified plants.Keywords: biological control, cry genes, entomopathogenic bacteria, lesser cornstalk borer. Seleção e caracterização de linhagens de Bacillus thuringiensis ativas contra Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)RESUMO. Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae) é considerada praga para mais de 60 espécies de plantas cultivadas como o milho, cana-de-açúcar, trigo, soja, arroz, feijão, sorgo, amendoim e algodão. O objetivo deste trabalho foi selecionar e caracterizar isolados de Bacillus thuringiensis virulentos a E. lignosellus atuando como método alternativo de controle. Assim, 47 isolados foram avaliados em bioensaios seletivos contra lagartas de 1º ínstar de E. lignosellus. Para os isolados mais tóxicos, a concentração letal (CL 50 ) foi estimada e a sua caracterização foi realizada por métodos morfológicos, bioquímicos e moleculares. Dos 47 isolados testados, 12 causaram mortalidade acima 85% e apresentaram CL 50 entre 0.038E+8 a 0.855E+8 esporos mL -1 . Os menores valores de CL 50 foram obtidos pelos isolados BR83, BR145, BR09, BR78, S1534 e S1302, os quais não diferiram da linhagem padrão HD-1, com exceção do BR83. O perfil proteico revelou proteínas Cry entre 60 e 130 kDa, e a caracterização molecular mostrou a presença dos genes cry1, cry2, cry3 e cry11. A análise morfológica identificou três diferentes inclusões cristalinas: bipiramidais, esféricas e cuboides. Entre os isolados avaliados, 12 apresentam potencial biotecnológico para controle de E. lignosellus via formulação de novos bioinseticida ou produção de plantas transgênicas.Palavras-chave: controle biológico, genes cry...

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“…Insecticides are the most commonly utilized control strategy but their use has not given effective control of S. frugiperda (Cruz 1997) and, therefore, integrated pest management strategies using predators are under investigation (Batista Filho et al 2003). Direct observation (Valicente & Barreto 1999, Oliveira et al 2004 and the use of entomopathogenic agents (Monnerat et al 2007) have been used in the search for natural enemies of S. frugiperda.…”

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confidence: 99%

Use of serological techniques for determination of Spodoptera frugiperda(J E Smith) predators (Lepidoptera: Noctuidae)

Santos-Neto¹,

Mezêncio²,

Chagas³

et al. 2010

Neotrop. entomol.

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-Spodoptera frugiperda (J E Smith) is an important pest of several crops, but especially on maize in Brazil. The implementation of biological control measures hinges on the identifi cation of its predators and other natural enemies. As a means of identifying predators, antibodies against S. frugiperda eggs were generated by inoculating rabbits with macerated S. frugiperda eggs, and the production of antibodies against S. frugiperda egg proteins was verifi ed by double immunodiffusion (DID). These antibodies were then utilized in another serological technique, counterimmunoeletrophoresis (CIE), to identify insects that could have ingested S. frugiperda eggs. Macerates of entire insects collected in maize plantations and of individual parts of their digestive tract, including the crop, were the source of antigens in the CIE, while predators fed S. frugiperda eggs in the laboratory served as the control. Antibodies produced by the inoculated rabbits were effective in detecting S. frugiperda egg proteins, especially if crop macerates were used as antigens. Among the species of insects collected from maize plantations, Lagria villosa Fabricius (Coleoptera: Lagriidae) and a species of Lygaeidae (Hemiptera) were identifi ed as possible S. frugiperda predators.

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Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis

Cited by 121 publications

References 14 publications

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

<b>Isolation and characterization of <i>Bacillus thuringiensis</i> strains active against <i>Elasmopalpus lignosellus</i> (Zeller, 1848) (Lepidoptera, Pyralidae)

Use of serological techniques for determination of Spodoptera frugiperda(J E Smith) predators (Lepidoptera: Noctuidae)

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