Screening of Blood Donors for Human Parvovirus B19 and Characterization of the Results

Wakamatsu, C.; Takakura, Fumihiro; Kojima, Eijiro; Kiriyama, Y.; Goto, N; Matsumoto, Kimikazu; Oyama, Minori; Sato, Hiroyuki; Okochi, Kazuo; Maeda, Yoshiaki

doi:10.1046/j.1423-0410.1999.7610014.x

Cited by 44 publications

(27 citation statements)

References 24 publications

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“…Despite the development and presence of B19-specific immune reactions, ϳ20% of all B19 infections show a prolonged state of viremia or viral persistence restricted to the synovial fluid, and viral genomes are detected in bone marrow or other organs, e.g., synovial tissue, liver, or myocardium, for several years after infection (4,8,12,13,19,27,(29)(30)(31)(32)(33). Compared with our age-matched control group and compared with healthy adult blood donors, who have been shown to contain B19 DNA in 7% and in 0.1% up to 0.6% of cases, respectively (34)(35)(36), viral genomes were detected in 35% of arthritis patients in the present study. In those cases in which DNA sequence analysis after PCR amplification was successful, parvovirus B19 genotype 1 genome sequences with minor nucleotide and amino acid changes were detected.…”

Section: Discussionmentioning

confidence: 84%

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Lehmann¹,

Knöll²,

Küster³

et al. 2003

Arthritis & Rheumatism

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Objective. To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases.Methods. Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG-complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR.Results. Twenty-six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n ‫؍‬ 22 [30%]) and/or the synovial fluid (n ‫؍‬ 16 [22%]), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty-six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1-specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2-specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed lowdegree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples.Conclusion. Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age-matched controls.

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Section: Discussionmentioning

confidence: 84%

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Lehmann¹,

Knöll²,

Küster³

et al. 2003

Arthritis & Rheumatism

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“…Numerous studies of the occurrence of viral DNA and specific antibodies in the general population have been described (1,7,11,14,17,(22)(23)(24). Simultaneous examination of BM and serum samples from patients for the presence of B19 DNA has re- vealed discrepancies, with B19 being briefly or continuously detected in BM following clearance of virus in the serum.…”

Section: B19 Is a Ubiquitous Infectious Agent And Is Transmitted By Tmentioning

confidence: 99%

“…The frequency of B19 viremia in voluntary blood donors has been estimated at rates of 1:167 to 1:35,000 (11,14,17,(22)(23)(24). As opposed to the many studies on B19 serology and viremia, information on the presence of B19 DNA in the BM of healthy individuals is limited.…”

mentioning

confidence: 99%

Prevalence of Parvovirus B19 and Parvovirus V9 DNA and Antibodies in Paired Bone Marrow and Serum Samples from Healthy Individuals

Heegaard

Petersen²,

Heilmann

et al. 2002

J Clin Microbiol

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Parvovirus B19 (hereafter referred to as B19) exhibits a marked tropism to human bone marrow (BM), and infection may lead to erythema infectiosum, arthropathy, hydrops fetalis, and various hematologic disorders. Recently, a distinct parvovirus isolate termed V9 with an unknown clinical spectrum was discovered. In contrast to the many studies of B19 serology and viremia, valid information on the frequency of B19 or V9 DNA in the BM of healthy individuals is limited. To develop a reference value, paired BM and serum samples from healthy subjects were tested for the presence of B19 and V9 DNA and specific antibodies. Immunoglobulin M (IgM) was not found in any of the serum samples. The prevalence of IgG showed a gradual and steady increase from 37% in children aged 1 to 5 years to 87% in people aged >50 years. When 190 well-characterized subjects were examined, B19 DNA was detected in the BM of 4 individuals (2.1%; 95% confidence interval, 0.58 to 5.3%) while none of the paired serum samples showed evidence of circulating viral DNA. V9 DNA was not found in any of the BM or serum samples. The finding of B19 DNA probably indicated a primary infection in one 7-year-old individual and reinfection or reactivation of persistent infection in the remaining three persons, aged 47 to 58 years. Serving as a benchmark for future studies, these findings are useful when interpreting epidemiologic data, performing BM transplantation, or considering clinical implications of parvovirus infection.Human parvovirus B19 (hereafter referred to as B19) is a member of the genus Erythrovirus of the family Parvoviridae (18). B19 exhibits a marked tropism to human bone marrow (BM) and replicates only in erythroid progenitor cells (3). Infection may lead to erythema infectiosum, arthropathy, hydrops fetalis, and various hematologic disorders, including aplastic crisis, chronic anemia, and idiopathic thrombocytopenic purpura (3). Diagnosis of B19 relies on serology and the detection of viral DNA by PCR or dot blot analysis. A possible emerging parvovirus isolate termed V9 with an unknown clinical spectrum and markedly different from B19 (Ͼ11% nucleotide disparity) was recently discovered (19). Sequencing, combined with PCR studies, has since demonstrated the need for specific and differentiated techniques when examining samples for possible B19 or V9 viremia (11). Conversely, cloning and production of the V9 capsid proteins and subsequent enzymelinked immunosorbent assay studies have revealed a 100% serologic cross-reactivity between the B19 and V9 isolates (13).The prevalence of immunoglobulin G (IgG) antibodies directed against B19 ranges from 15 to 60% in children 6 to 19 years old: and from 30 to 60% in adults and is more than 85% in the geriatric population (1,7,22). The frequency of B19 viremia in voluntary blood donors has been estimated at rates of 1:167 to 1:35,000 (11,14,17,(22)(23)(24). As opposed to the many studies on B19 serology and viremia, information on the presence of B19 DNA in the BM of healthy individuals is limited. Thou...

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“…Esta frecuencia se encuentra entre las más altas reportadas previamente en la literatura para este tipo de poblaciones y es cercana a la descrita por Yoto y cols, quienes notifi caron una frecuencia de 1 en 167 y Candotti y cols, que encontraron un rango 0,55 a 1,3% 11,18 . No obstante, otros autores han documentado presencia de ADN de B19 en un amplio rango de frecuencias, las que fl uctúan entre 1 en 35.000 y 1 en 625 10,[19][20][21][22] . Estas diferencias serían consecuencia de las distintas técnicas utilizadas y posiblemente, debido también a variables epidemiológicas asociadas a las diferentes poblaciones estudiadas 2 .…”

Section: Discussionunclassified

Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

et al. 2011

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Screening of Blood Donors for Human Parvovirus B19 and Characterization of the Results

Abstract: These results suggest that the RHA(+) RHA inhibition (+) sera were infectious in vitro. The combination of RHA and the RHA inhibition test is considered to be useful for the large-scale screening of infectious B19 virus in blood donors with high specificity.

Cited by 44 publications

References 24 publications

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Prevalence of Parvovirus B19 and Parvovirus V9 DNA and Antibodies in Paired Bone Marrow and Serum Samples from Healthy Individuals

Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

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